Infrared pulsed fiber laser-produced silver-109-nanoparticles for laser desorption/ionization mass spectrometry of carboxylic acids

Carbon-Based Nanoparticles and Their Surface-Modified Counterparts as MALDI Matrices

“…Metallic nanoparticles are another major group of nanoparticles used as MALDI matrices. The main constituent in the structures of these nanosized matrices can be a variety of particles, such as gold, silver, iron oxide, or titanium dioxide. − The main structural differences between metal nanoparticles and carbon-based ones are the high variability in the crystal structures of the metal-based nanoparticles compared to the more homogeneous structure of the carbon-based nanoparticles. Metal-based nanoparticles have been combined with other compounds to improve their physicochemical properties, increasing their efficiency as MALDI matrices. − Silica particles, for example, have been coated with gold nanoparticles and used as a matrix for MALDI imaging of a series of low molecular weight compounds, such as amino acids, peptides, and small carbohydrates in a variety of plant and animal tissues .…”

Laser Generated Gold Nanoparticles for Mass Spectrometry of Low Molecular Weight Compounds

“…Due to its soft ionization potential, MALDI is one of the most selective, sensitive and efficient mass spectrometry methods. This allows it to be widely used for the analysis of ionic high-molecular weight compounds such as peptides, proteins [2], synthetic polymers [3] or polysaccharides [4], but it is also possible to detect low molecular weight (LMW) compounds such as lipids [5,6]. However, a disadvantage of MALDI which prevents it from being used too frequently for the detection of LMW compounds (MW <1000 Da) is the need to use MALDI matrices, which are low molecular weight organic acids that generate a variety of matrix-bound ions during the desorption/ionization process.…”

“…In part, the problems outlined have been resolved by developing the surface ionization assisted mass spectrometry (SALDI) technique. SALDI uses target plates coated with various nanostructures [6,10,11]. The nanoparticles used simplify the mass spectrum by reducing spectral interferences.…”

Nanostructured Layer of Silver for Detection of Small Biomolecules in Surface-Assisted Laser Desorption Ionization Mass Spectrometry

“…The results showed 93% sensitivity and 91% specificity in the differentiation between cancer and controls in the internal validation group compared to 84% sensitivity and specificity in the external validation group, and up to eight biomarkers were distinguished. Kołodziej et al reported the application of silver-109 nanoparticles for the detection of carboxylic acids such as azelaic, 3-methylhippuric, linoleic, oleic, arachidic and erucic acids [18]. 109 AgNPs were prepared using pulsed fiber laser (PLF) 2D GS (galvano-scanner) LASiS (laser ablation synthesis in solution) and were applied for the detection of carboxylic acids in spiked human blood serum to assess the matrix effect and semi-automatic mass spectrometry imaging [18].…”

“…Kołodziej et al reported the application of silver-109 nanoparticles for the detection of carboxylic acids such as azelaic, 3-methylhippuric, linoleic, oleic, arachidic and erucic acids [18]. 109 AgNPs were prepared using pulsed fiber laser (PLF) 2D GS (galvano-scanner) LASiS (laser ablation synthesis in solution) and were applied for the detection of carboxylic acids in spiked human blood serum to assess the matrix effect and semi-automatic mass spectrometry imaging [18]. Mesoporous PdPtAu spheres were utilized to reveal the specific metabolic phenotype of gastric cancer and resulted in precise diagnoses with an area under the curve of 0.942 [19].…”