Infrared Microscopy and Imaging of Hard and Soft Tissues

Menedelsohn, Richard; Boskey, Adele L.; Camacho, Nancy P.

doi:10.1002/9780470988541.ch11

Cited by 2 publications

(2 citation statements)

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“…4). IR imaging of bone biopsies thus becomes useful for diagnosing the quality of bone, above and beyond what can be learned from routine histologic studies [70][71][72].…”

Section: Characterizing the Mineral And Matrix In Bone Diseasesmentioning

confidence: 99%

Infrared spectroscopic characterization of mineralized tissues

Boskey

Mendelsohn

2005

Vibrational Spectroscopy

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117

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Vibrational spectroscopy (Infrared and Raman), and in particular micro-spectroscopy and micro-spectroscopic imaging has been used to characterize developmental changes in bone and other mineralized tissues, to monitor these changes in cell cultures, and to detect disease and drug-induced modifications. Examples of the use of infrared micro-spectroscopy and micro-spectroscopic imaging are discussed in this review.

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“…4). IR imaging of bone biopsies thus becomes useful for diagnosing the quality of bone, above and beyond what can be learned from routine histologic studies [70][71][72].…”

Section: Characterizing the Mineral And Matrix In Bone Diseasesmentioning

confidence: 99%

Infrared spectroscopic characterization of mineralized tissues

Boskey

Mendelsohn

2005

Vibrational Spectroscopy

Self Cite

138

117

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“…These studies have typically relied on a transflection or transmission sampling method for analysis. 9–11 Recently, efforts to incorporate infrared microanalysis into a protocol for disease detection have settled on the use of reflective substrates, most commonly low-E glass slides, in conjunction with transflection spectroscopy. Although this approach is a useful method for tissue analysis, it can pose several limitations.…”

Section: Introductionmentioning

confidence: 99%

The Advantages of an Attenuated Total Internal Reflection Infrared Microspectroscopic Imaging Approach for Kidney Biopsy Analysis

et al. 2010

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The benefits of an ATR-FTIR imaging approach for kidney biopsy analysis are described. Biopsy sections collected from kidney stone formers are analyzed at the initial stages of stone development to provide insights into stone growth and formation. The majority of tissue analysis currently conducted with IR microspectroscopy is performed with a transflection method. The research presented in this manuscript demonstrates that ATR overcomes many of the disadvantages of transflection or transmission measurements for tissue analysis including an elimination of spectral artifacts. When kidney biopsies with small mineral inclusions are analyzed with a transflection approach, specular reflection, and the Christiansen effect (anomalous dispersion) can occur leading to spectral artifacts. Another effect specific to the analysis of mineral inclusions present in kidney biopsies is known as the reststrahlen effect where the inclusions become strong reflectors near an absorption band. ATR eliminates these effects by immersing the sample in a high index medium. Additionally, the focused beam size for ATR is decreased by a factor of four when a germanium internal reflection element is used, allowing the acquisition of spectra from small mineral inclusions several micrometers in diameter. If quantitative analysis of small mineral inclusions is ultimately desired, ATR provides the photometrically accurate spectra necessary for quantification.

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Infrared Microscopy and Imaging of Hard and Soft Tissues

Cited by 2 publications

References 65 publications

Infrared spectroscopic characterization of mineralized tissues

Infrared spectroscopic characterization of mineralized tissues

The Advantages of an Attenuated Total Internal Reflection Infrared Microspectroscopic Imaging Approach for Kidney Biopsy Analysis

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