Infrared Mass Spectrometric Imaging below the Diffraction Limit

Luxembourg, S.L.; McDonnell, Liam A.; Mize, Todd H.; Heeren, Ron M. A.

doi:10.1021/pr049762+

Cited by 37 publications

(42 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The desorbed ions within a large laser spot (150 m × 200 m) retain their original spatial distribution during time-of-flight (ToF) separation and are detected by a position sensitive detector. The resulting stigmatic images at highest magnification have a pixel size of 500 nm and a resolving power of 4 m, both for UV and IR lasers [10,11]. With the mass microscope we approach the spatial resolution obtained by secondary ion mass spectrometry (SIMS) but with the ability to desorb large organic molecules like peptides and proteins.…”

Section: Introductionmentioning

confidence: 99%

High-resolution MALDI imaging mass spectrometry allows localization of peptide distributions at cellular length scales in pituitary tissue sections

Altelaar

Taban

McDonnell

et al. 2007

International Journal of Mass Spectrometry

Self Cite

106

View full text Add to dashboard Cite

Matrix assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) has been used to determine peptide distributions directly from rat, mouse and human pituitary tissue sections. Since these organs are small (10 2 -10 3 m) the spatial resolution of IMS is a key issue in molecular imaging of pituitary tissue sections. Here we show that high-resolution IMS allows localization of neuropeptide distributions within different cell clusters of a single organ of a pituitary tissue section. The sample preparation protocol does not result in analyte redistribution and is therefore applicable to IMS experiments at cellular length scales. The stigmatic imaging mass spectrometer used in this study produces selected-ion-count images with pixel sizes of 500 nm and a resolving power of 4 m, yielding superior spatial detail compared to images obtained in microprobe imaging experiments. Furthermore, we show that with imaging mass spectrometry a distinction can be made between different mammalian tissue sections based on differences in the amino acid sequence of neuropeptides with the same function. This example demonstrates the power of IMS for label-free molecular imaging at relevant biological length scales.

show abstract

Section: Introductionmentioning

confidence: 99%

High-resolution MALDI imaging mass spectrometry allows localization of peptide distributions at cellular length scales in pituitary tissue sections

Altelaar

Taban

McDonnell

et al. 2007

International Journal of Mass Spectrometry

Self Cite

106

View full text Add to dashboard Cite

show abstract

“…An example is the use of IR-MALDI imaging, with resolutions below the diffraction limit of the optical system used. It allows IR imaging with identical spatial resolution as UV-based MALDI imaging, even though the wavelength is an order of magnitude longer [13].…”

Section: Spatial Resolution: Microscope Mode Imaging Msmentioning

confidence: 99%

Imaging mass spectrometry: Hype or hope?

Heeren

Smith

Stauber

et al. 2009

J. Am. Soc. Mass Spectrom.

Self Cite

117

100

View full text Add to dashboard Cite

Imaging mass spectrometry is currently receiving a significant amount of attention in the mass spectrometric community. It offers the potential of direct examination of biomolecular patterns from cells and tissue. This makes it a seemingly ideal tool for biomedical diagnostics and molecular histology. It is able to generate beautiful molecular images from a large variety of surfaces, ranging from cancer tissue sections to polished cross sections from old-master paintings. What are the parameters that define and control the implications, challenges, opportunities, and (im)possibilities associated with the application of imaging MS to biomedical tissue studies. Is this just another technological hype or does it really offer the hope to gain new insights in molecular processes in living tissue? In this critical insight this question is addressed through the discussion of a number of aspects of MS imaging technology and sample preparation that strongly determine the outcome of imaging MS experiments.

show abstract

“…In this method, the spatial resolution obtained in MALDI-IMS is comparable to that obtained in secondary ion mass spectrometry (SIMS) imaging experiments of tissue sections. Using relatively large laser spots (150 Â 200 mm 2 ), which is favorable for MALDI efficiency, delivers an image quality of 500-nm pixel size and 4-mm resolving power in both IR and UV MALDI-IMS 36,37 .…”

Section: Mass Microscopementioning

confidence: 99%

Imaging mass spectrometry at cellular length scales

et al. 2007

View full text Add to dashboard Cite

Imaging mass spectrometry (IMS) allows the direct investigation of both the identity and the spatial distribution of the molecular content directly in tissue sections, single cells and many other biological surfaces. In this protocol, we present the steps required to retrieve the molecular information from tissue sections using matrix-enhanced (ME) and metal-assisted (MetA) secondary ion mass spectrometry (SIMS) as well as matrix-assisted laser desorption/ionization (MALDI) IMS. These techniques require specific sample preparation steps directed at optimal signal intensity with minimal redistribution or modification of the sample analytes. After careful sample preparation, different IMS methods offer a unique discovery tool in, for example, the investigation of (i) drug transport and uptake, (ii) biological processing steps and (iii) biomarker distributions. To extract the relevant information from the huge datasets produced by IMS, new bioinformatics approaches have been developed. The duration of the protocol is highly dependent on sample size and technique used, but on average takes approximately 5 h.

show abstract

Infrared Mass Spectrometric Imaging below the Diffraction Limit

Cited by 37 publications

References 14 publications

High-resolution MALDI imaging mass spectrometry allows localization of peptide distributions at cellular length scales in pituitary tissue sections

High-resolution MALDI imaging mass spectrometry allows localization of peptide distributions at cellular length scales in pituitary tissue sections

Imaging mass spectrometry: Hype or hope?

Imaging mass spectrometry at cellular length scales

Contact Info

Product

Resources

About