Infrared Light-Emitting Diode (LED) Effects on Orthodontic Tooth Movement

Abstract

The present study aimed to analyze the effect of LED phototherapy on the presence of hyalinization and root resorption during orthodontic tooth movement (OTM) in rats and to measure the amount of tooth movement. Eighty rats were allocated into two groups: LED and control (CON), where the LED rats were irradiated with infrared LED (850 nm, 30 mW) for 5 min during the first five days of OTM and where controls were not irradiated. Both groups were subdivided into four subgroups (n=10) according to the date of euthanasia (4, 7, 14 and 21 days). Five out of ten LED21 and five of ten CON21 rats were submitted to micro-computed tomography (μCT); μCT scans were taken on days 0, 7, 14 and 21. For histological study, maxillae were processed to light microscopy using Hematoxylin-Eosin (HE) and Tartrate-Resistant Acid Phosphatase (TRAP) histochemistry. The amount of tooth movement did not differ between LED and CON. Hyalinization was observed at the pressure areas in both groups, and it did not show a statistically significant difference between the groups. Root resorption was also observed in both groups after 7 days and it did not represent any differences between the two groups. LED phototherapy was not able to increase the amount of OTM. Similar characteristics of hyalinization and root resorption were observed in both groups.

Automated tooth segmentation as an innovative tool to assess 3D-tooth movement and root resorption in rodents

“…3D evaluation premises reliable image segmentation which can be particularly challenging when metal artifacts are present, or when histograms overlap. [16][17][18][19][20] Since mineral contents of cementum and bone were reported to be likewise [21], reliable approaches to segment tooth roots in μCT are an inevitable premise for volumetric assessment of OTM and RR in μCT.…”

Hyalinization and Molecular Pathways Involved in Orthodontic Tooth Movement: A Systematic Review and Meta-Analysis

“…Primarily established on histological investigations, pressure, and a tension side have also been recognized during the OTM procedure [7,8,10]. Hyalinization can be accordingly sterile necrosis at the pressure region of PDL observed amid the early phases of the OTM, and broad hyaline zones can thus result in imperative delay within the tooth movement procedure [7,11,12]. In related studies, hyalinization has been seemingly found within the initial phase of OTM, and small hyalinized patches have been additionally observed within the afterward phases [13,14].…”