Infrared imaging of sub-retinal structures in the human ocular fundus

Elsner, Ann E.; Burns, Stephen A.; Weiter, John J.; Delori, François C.

doi:10.1016/0042-6989(95)00100-e

Cited by 285 publications

(283 citation statements)

References 25 publications

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“…AOSLO has proven to be a powerful platform for translating existing microscopy and ophthalmoscopy methods for high-resolution in vivo imaging. Off-axis detection methods, such as "dark-field" (19), "offset-aperture" (20), and "split-detection" (21) have been adapted to AOSLO to image the retinal pigment epithelium (22), blood vessel walls (23), and putative cone inner segments (24). Split-detection resolved uncertainty about cone survival in achromatopsia (24) and may be useful for doing the same in AMD.…”

mentioning

confidence: 99%

Imaging individual neurons in the retinal ganglion cell layer of the living eye

Rossi

Granger

Sharma

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

171

151

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Although imaging of the living retina with adaptive optics scanning light ophthalmoscopy (AOSLO) provides microscopic access to individual cells, such as photoreceptors, retinal pigment epithelial cells, and blood cells in the retinal vasculature, other important cell classes, such as retinal ganglion cells, have proven much more challenging to image. The near transparency of inner retinal cells is advantageous for vision, as light must pass through them to reach the photoreceptors, but it has prevented them from being directly imaged in vivo. Here we show that the individual somas of neurons within the retinal ganglion cell (RGC) layer can be imaged with a modification of confocal AOSLO, in both monkeys and humans. Human images of RGC layer neurons did not match the quality of monkey images for several reasons, including safety concerns that limited the light levels permissible for human imaging. We also show that the same technique applied to the photoreceptor layer can resolve ambiguity about cone survival in age-related macular degeneration. The capability to noninvasively image RGC layer neurons in the living eye may one day allow for a better understanding of diseases, such as glaucoma, and accelerate the development of therapeutic strategies that aim to protect these cells. This method may also prove useful for imaging other structures, such as neurons in the brain.

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mentioning

confidence: 99%

Imaging individual neurons in the retinal ganglion cell layer of the living eye

Rossi

Granger

Sharma

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

171

151

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“…2,3,9,[11][12][13] The extended imaging field provided by the lens obviates the need for photomontages ( Figure 4), avoiding their potential limitations, which include skipped areas and local variations in contrast and magnification. The peripheral retina and choroid are imaged readily through a small patient pupil, and iris neovascularization can be documented easily.…”

Section: Resultsmentioning

confidence: 99%

Scanning Laser Ophthalmoscopy and Angiography With a Wide-Field Contact Lens System

et al. 2005

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To perform fluorescein and indocyanine green angiography for large or peripheral chorioretinal structures using a contact lens system that provides a 5-fold increase in the field of view of a confocal scanning laser ophthalmoscope (SLO).Methods: Separate handheld contact and noncontact ophthalmoscopic lenses were manually aligned with the optical axis of a confocal SLO to demonstrate the feasibility of wide-field SLO angiography. An integrated, widefield contact lens system was then designed and constructed to increase the SLO's 10°, 20°, and 30°imaging fields to 50°, 100°, and 150°, respectively.Results: Simultaneous fluorescein and indocyanine green angiography was performed with the integrated, widefield contact lens system for more than 50 patients with disorders that affect their peripheral retina and choroid. Retinal and choroidal abnormalities, including neovas-cularization and capillary nonperfusion, are easily detected and documented well beyond the range of conventional fundus cameras and SLOs. Peripheral retinal and choroidal hemodynamics can be readily observed and recorded.Conclusions: A confocal SLO has adequate resolution for clinically useful reflectance and angiographic imaging even when its field size is increased 5-fold by a widefield contact lens system. Dynamic and static wide-field angiography can be performed without the limitations of manual or computer-automated photomontages. Peripheral retinal conditions can be studied and recorded to confirm observations from indirect ophthalmoscopy and to facilitate retinal photocoagulation and vitreoretinal surgery.

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“…On the contrary, red and nearinfrared penetrate deep into the uveal layers and may return towards the pupil after having been multiply scattered. These wavelengths are optimal for defining subretinal structures (Elsner et al 1996), notably when CLSO optics are used.…”

Section: Commentsmentioning

confidence: 99%

Enhanced visualisation of acute macular neuroretinopathy by spectral imaging

Tingsgaard

Sander

Larsen

1999

Acta Ophthalmologica Scandinavica

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ABSTRACT.Purpose: To report photographic findings from a case of acute macular neuroretinopathy. Methods: Fundus photography on color transparencies, red-free digital CCDrecording, and laser-scanning ophthalmoscopy with monochromatic red and blue illumination. Results: The visibility of the retinal lesions was markedly enhanced by narrowing the illumination bandwidth and by the stray light suppression of the confocal laser scanning ophthalmoscope. Conclusions: The diagnosis of acute macular neuroretinopathy, and possibly other chorioretinal diseases as well, can be facilitated by multispectral imaging and stray-light reduction optics.

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Infrared imaging of sub-retinal structures in the human ocular fundus

Cited by 285 publications

References 25 publications

Imaging individual neurons in the retinal ganglion cell layer of the living eye

Imaging individual neurons in the retinal ganglion cell layer of the living eye

Scanning Laser Ophthalmoscopy and Angiography With a Wide-Field Contact Lens System

Enhanced visualisation of acute macular neuroretinopathy by spectral imaging

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