Infrared and 2-Dimensional Correlation Spectroscopy Study of the Effect of CH3NH3PbI3 and CH3NH3SnI3 Photovoltaic Perovskites on Eukaryotic Cells

Abstract

We studied the effect of the exposure of human A549 and SH-SY5Y cell lines to aqueous solutions of organic/inorganic halide perovskites CH3NH3PbI3 (MAPbI3) and CH3NH3SnI3 (MASnI3) at the molecular level by using Fourier transform infrared microspectroscopy. We monitored the infrared spectra of some cells over a few days following exposure to the metals and observed the spectroscopic changes dominated by the appearance of a strong band at 1627 cm−1. We used Infrared (IR) mapping to show that this change was associated with the cell itself or the cellular membrane. It is unclear whether the appearance of the 1627 cm−1 band and heavy metal exposure are related by a direct causal relationship. The spectroscopic response of exposure to MAPbI3 and MASnI3 was similar, indicating that it may arise from a general cellular response to stressful environmental conditions. We used 2D correlation spectroscopy (2DCOS) analysis to interpret spectroscopic changes. In a novel application of the method, we demonstrated the viability of 2DCOS for band assignment in spatially resolved spectra. We assigned the 1627 cm−1 band to the accumulation of an abundant amide or amine containing compound, while ruling out other hypotheses. We propose a few tentative assignments to specific biomolecules or classes of biomolecules, although additional biochemical characterization will be necessary to confirm such assignments.

Metal‐Free Perovskite Piezoelectric Nanogenerators for Human–Machine Interfaces and Self‐Powered Electrical Stimulation Applications

“…The results indicated that the higher concentration of perovskite material inside the cell medium, the lower cell viability rate observed. [ 58 ] As mentioned above, the role of B‐site Pb 2+ cation was replaced by the NH 4+ cation in MDABCO‐NH 4 I 3 to reduce toxicity, as illustrated in Figure 5 a . To further assess the cytotoxicity of the as‐synthesized MDABCO‐NH 4 I 3 film, a cell viability test has been conducted by using L929 fibroblasts.…”

Imaging and spectroscopy of domains of the cellular membrane by photothermal-induced resonance

“…6 The two peaks are typically assigned to the Amide I and Amide II modes of the amide group of polypeptide chains, although some amino acid side chains, lipids and other molecules can also contribute. 43 One difference from cellular far-field spectra, and from protein spectra in general, is the relative intensity of the two peaks. This is comparable in Figure 5C, while in far-field IR spectra the Amide I peak is markedly more intense than the Amide II peak.…”

Substitution of Ethylammonium Halides Enabling Lead-Free Tin-Based Perovskite Solar Cells with Enhanced Efficiency and Stability

“…24−26 1, low-dimensional perovskite structures are preferably formed. 21,27,28 Some organic cations with a large size, such as phenethylammonium (PEA + ), 11,29 ethylenediammonium (EDA 2+ ), 15 butylammonium (BA + ), 30,31 and allylammonium (ALA + ), 32 have been introduced to replace A cation to make efficient Sn PSCs. Li et al added PEACl into FASnI 3 to suppress the oxidation of Sn 2+ and induce perovskite crystallization perpendicular to the substrate, achieving a power conversion efficiency (PCE) of 9.1%.…”

“…The substitution of organic cations or halogen anions are effective to improve the performance of Sn-based PSCs. − Although there are no convenient rules to guide the selection of organic salts, the structural stability of perovskites is the primary consideration. Typically, the perovskite (ABX 3 ) with a Goldschmidt tolerance factor approaching 1, determined by the size of A cation and the interaction between A cation and BX 6 octahedra, can obtain a relatively stable structure. − If the tolerance factor is beyond 1, low-dimensional perovskite structures are preferably formed. ,, …”