Infrapatellar Fat Pad/Synovium Complex in Early-Stage Knee Osteoarthritis: Potential New Target and Source of Therapeutic Mesenchymal Stem/Stromal Cells

Greif, Dylan N.; Kouroupis, Dimitrios; Murdock, Christopher J.; Griswold, Anthony J.; Kaplan, Lee D.; Best, Thomas M.; Correa, Diego

doi:10.3389/fbioe.2020.00860

Cited by 50 publications

(42 citation statements)

References 184 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…synovium, adipose, cartilage, ligament, and synovial fluid), that experience physical forces and diverse paracrine signaling events. The main actor in the progression of osteoarthritis is controversial, however a greater appreciation of the role adipose tissue (infrapatellar fat pad) plays in promoting osteoarthritis has recently garnered more interest ( Collins et al, 2021 ; Greif et al, 2020 ). Moreover, the combinatorial application of FGF-2 and FGF-8 in the knee joint was recently found to suppress adipokine secretion and demonstrated a decreased inflammatory profile ( Mengsteab et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Control of mesenchymal cell fate via application of FGF-8b in vitro

2021

View full text Add to dashboard Cite

In order to develop strategies to regenerate complex tissues in mammals, understanding the role of signaling in regeneration competent species and mammalian development is of critical importance. Fibroblast growth factor 8 (FGF-8) signaling has an essential role in limb morphogenesis and blastema outgrowth. Therefore, we aimed to study the effect of FGF-8b on the proliferation and differentiation of mesenchymal stem cells (MSCs), which have tremendous potential for therapeutic use of cell-based therapy. Rat adipose derived stem cells (ADSCs) and muscle progenitor cells (MPCs) were isolated and cultured in growth medium and various types of differentiation medium (osteogenic, chondrogenic, adipogenic, tenogenic, and myogenic medium) with or without FGF-8b supplementation. We found that FGF-8b induced robust proliferation regardless of culture medium. Genes related to limb development were upregulated in ADSCs by FGF-8b supplementation. Moreover, FGF-8b enhanced chondrogenic differentiation and suppressed adipogenic and tenogenic differentiation in ADSCs. Osteogenic differentiation was not affected by FGF-8b supplementation. FGF-8b was found to enhance myofiber formation in rat MPCs. Overall, this study provides foundational knowledge on the effect of FGF-8b in the proliferation and fate determination of MSCs and provides insight in its potential efficacy for musculoskeletal therapies.

show abstract

Section: Discussionmentioning

confidence: 99%

Control of mesenchymal cell fate via application of FGF-8b in vitro

2021

View full text Add to dashboard Cite

show abstract

“… 71 ) and the cartilage protective effects of the infrapatellar fat pad (IPFP) (reviewed in Ref. 72 ). However, the vast majority of the literature concerning paracrine actions of these depots covers their negative role in disease states.…”

Section: Adipose Tissue Diversity In Form and Functionmentioning

confidence: 99%

“… 92 ) but intriguingly, ADSCs isolated from this depot have also been shown to ameliorate the condition (reviewed in Ref. 72 ).…”

Section: Adipose Tissue Diversity In Form and Functionmentioning

confidence: 99%

Harnessing adipose stem cell diversity in regenerative medicine

2021

View full text Add to dashboard Cite

Since the first isolation of mesenchymal stem cells from lipoaspirate in the early 2000s, adipose tissue has been a darling of regenerative medicine. It is abundant, easy to access, and contains high concentrations of stem cells (ADSCs) exhibiting multipotency, proregenerative paracrine signaling, and immunomodulation—a winning combination for stem cell-based therapeutics. While basic science, preclinical and clinical findings back up the translational potential of ADSCs, the vast majority of these used cells from a single location—subcutaneous abdominal fat. New data highlight incredible diversity in the adipose morphology and function in different anatomical locations or depots. Even in isolation, ADSCs retain a memory of this diversity, suggesting that the optimal adipose source material for ADSC isolation may be application specific. This review discusses our current understanding of the heterogeneity in the adipose organ, how that heterogeneity translates into depot-specific ADSC characteristics, and how atypical ADSC populations might be harnessed for regenerative medicine applications. While our understanding of the breadth of ADSC heterogeneity is still in its infancy, clear trends are emerging for application-specific sourcing to improve regenerative outcomes.

show abstract

“…Some research groups suggest that to profile MSCs we must observe key parameters like CFU-F efficiency, MSC isolation, multilineage differentiation, immunomodulation, telomere length, trophic factor quantification, cumulative growth, and surface phenotype (Samsonraj et al, 2017). However, the ISCT guidelines are still the recommended criteria for the isolation and characterization of MSCs and are precise enough to combine the majority of the today existing knowledge, recognizing that future research, along with scientific breakthroughs, will probably lead to optimization of the criteria (Greif et al, 2020;Kurenkova et al, 2020).…”

Section: Characterization and Phenotype Of Mscsmentioning

confidence: 99%

Mesenchymal Stem Cells (MSCs) as a Potential Therapeutic Strategy in COVID-19 Patients: Literature Research

Coelho

Alvites

Branquinho

et al. 2020

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

In 2019, an outbreak of an unknown coronavirus-SARS-CoV-2-responsible for COVID-19 disease, was first reported in China, and evolved into a pandemic of huge dimensions and raised serious concerns for global health. The number of critical cases continues to increase dramatically, while vaccines and specific treatments are not yet available. There are several strategies currently being studied for the treatment of adverse symptoms of COVID-19, that encompass Acute Lung Injury (ALI)/Acute Respiratory Distress Syndrome (ARDS), extensive pulmonary inflammation, cytokine storm, and pulmonary edema, due to virus-induced pneumonia. Mesenchymal stem cells (MSCs) are at the origin of new revolutionary treatments, which may come to be applied in such as Regenerative Medicine, Immunotherapy, Tissue Engineering, and Cell and Molecular Biology due to immunomodulation and anti-inflammatory activity. MSCs have already been studied with positive outcomes for other lung pathologies, thus representing and being identified as an important opportunity for the treatment of COVID-19. It has recently been shown that these cells allow hopeful and effective therapies for serious or critical COVID-19, minimizing its adverse symptoms. In this study we will analyze the MSCs, their origin, differentiation, and therapeutic potential, making a bridge with the COVID-19 disease and its characteristics, as a potential therapeutic strategy but also reporting recent studies where these cell-based therapies were used for the treatment of COVID-19 patients.

show abstract

Infrapatellar Fat Pad/Synovium Complex in Early-Stage Knee Osteoarthritis: Potential New Target and Source of Therapeutic Mesenchymal Stem/Stromal Cells

Cited by 50 publications

References 184 publications

Control of mesenchymal cell fate via application of FGF-8b in vitro

Control of mesenchymal cell fate via application of FGF-8b in vitro

Harnessing adipose stem cell diversity in regenerative medicine

Mesenchymal Stem Cells (MSCs) as a Potential Therapeutic Strategy in COVID-19 Patients: Literature Research

Contact Info

Product

Resources

About