INFRAFRONTIER quality principles in systemic phenotyping

Ehlich, Hilke; Cater, Heather; Flenniken, Ann M.; Mura, Anne-Marie; Ntafis, Vasileios; Raess, Michael; Selloum, Mohammed; Stoeger, Claudia; Suchanova, Sarka; Vuolteenaho, Reetta; Brown, Steve; Hérault, Yann; Hinttala, Reetta; Angelis, Martin Hrabé de; Kollias, George; Kontoyiannis, Dimitris L.; Malissen, Bernard; McKerlie, Colin; Sedláček, Radislav; Wells, Sara; Zarubica, Ana; Rozman, Jan; Sorg, Tania

doi:10.1007/s00335-021-09892-2

Cited by 4 publications

(2 citation statements)

References 9 publications

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“…The number of genetically engineered mice with cardiac phenotypes strongly increased during the last years and their cardiovascular physiology needs to be characterized before they are used for dedicated research questions (Birling et al 2021 ; James et al 1998 ; Svenson et al 2003 ). Cardiac phenotyping requires reproducible, high-throughput methods, which should be conducted in an accurate and standardized manner making sure that findings are not missed, anticipated or biased (Ehlich et al 2022 ; Hoit and Nadeau 2001 ; Hoit 2004 ). Many phenotypes are subtle, and mice are often able to compensate for mutations in order to maintain blood pressure and cardiac output within a healthy range (Kass et al 1998 ).…”

Section: Introductionmentioning

confidence: 99%

What to consider for ECG in mice—with special emphasis on telemetry

Calvet

Seebeck

2023

Mamm Genome

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Genetically or surgically altered mice are commonly used as models of human cardiovascular diseases. Electrocardiography (ECG) is the gold standard to assess cardiac electrophysiology as well as to identify cardiac phenotypes and responses to pharmacological and surgical interventions. A variety of methods are used for mouse ECG acquisition under diverse conditions, making it difficult to compare different results. Non-invasive techniques allow only short-term data acquisition and are prone to stress or anesthesia related changes in cardiac activity. Telemetry offers continuous long-term acquisition of ECG data in conscious freely moving mice in their home cage environment. Additionally, it allows acquiring data 24/7 during different activities, can be combined with different challenges and most telemetry systems collect additional physiological parameters simultaneously. However, telemetry transmitters require surgical implantation, the equipment for data acquisition is relatively expensive and analysis of the vast number of ECG data is challenging and time-consuming. This review highlights the limits of non-invasive methods with respect to telemetry. In particular, primary screening using non-invasive methods can give a first hint; however, subtle cardiac phenotypes might be masked or compensated due to anesthesia and stress during these procedures. In addition, we detail the key differences between the mouse and human ECG. It is crucial to consider these differences when analyzing ECG data in order to properly translate the insights gained from murine models to human conditions.

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Section: Introductionmentioning

confidence: 99%

What to consider for ECG in mice—with special emphasis on telemetry

Calvet

Seebeck

2023

Mamm Genome

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“…These include generation of precision mouse or rat models, germ-free derivation of mouse models for gnotobiotic studies, systemic phenotyping for broader phenotypic analysis, late onset phenotyping for ageing-related disorders, and more. To improve reproducibility and replicability of animal studies, INFRAFRONTIER has also developed and published quality principles for systemic phenotyping that include 11 key principles to establish a basis for reproducible and reliable research results when it comes to phenotyping of mouse models (Ehlich et al 2022 ). Recently, INFRAFRONTIER has developed and launched new services and designed dedicated landing pages bringing together resources for researchers from specific disease areas.…”

Section: Introductionmentioning

confidence: 99%

INFRAFRONTIER: mouse model resources for modelling human diseases

et al. 2023

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Over the last decade, INFRAFRONTIER has positioned itself as a world-class Research Infrastructure for the generation, phenotyping, archiving, and distribution of mouse models in Europe. The INFRAFRONTIER network consists of 22 partners from 15 countries, and is continuously enhancing and broadening its portfolio of resources and services that are offered to the research community on a non-profit basis. By bringing together European rodent model expertise and providing valuable disease model services to the biomedical research community, INFRAFRONTIER strives to push the accessibility of cutting-edge human disease modelling technologies across the European research landscape. This article highlights the latest INFRAFRONTIER developments and informs the research community about its extensively utilised services, resources, and technical developments, specifically the intricacies of the INFRAFRONTIER database, use of Curated Disease Models, overview of the INFRAFRONTIER Cancer and Rare Disease resources, and information about its main state-of-the-art services. Graphical abstract

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SRC and TKS5 mediated podosome formation in fibroblasts promotes extracellular matrix invasion and pulmonary fibrosis

Barbayianni,

Kanellopoulou,

Fanidis

et al. 2023

Nat Commun

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The activation and accumulation of lung fibroblasts resulting in aberrant deposition of extracellular matrix components, is a pathogenic hallmark of Idiopathic Pulmonary Fibrosis, a lethal and incurable disease. In this report, increased expression of TKS5, a scaffold protein essential for the formation of podosomes, was detected in the lung tissue of Idiopathic Pulmonary Fibrosis patients and bleomycin-treated mice. Τhe profibrotic milieu is found to induce TKS5 expression and the formation of prominent podosome rosettes in lung fibroblasts, that are retained ex vivo, culminating in increased extracellular matrix invasion. Tks5+/- mice are found resistant to bleomycin-induced pulmonary fibrosis, largely attributed to diminished podosome formation in fibroblasts and decreased extracellular matrix invasion. As computationally predicted, inhibition of src kinase is shown to potently attenuate podosome formation in lung fibroblasts and extracellular matrix invasion, and bleomycin-induced pulmonary fibrosis, suggesting pharmacological targeting of podosomes as a very promising therapeutic option in pulmonary fibrosis.

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INFRAFRONTIER quality principles in systemic phenotyping

Cited by 4 publications

References 9 publications

What to consider for ECG in mice—with special emphasis on telemetry

What to consider for ECG in mice—with special emphasis on telemetry

INFRAFRONTIER: mouse model resources for modelling human diseases

SRC and TKS5 mediated podosome formation in fibroblasts promotes extracellular matrix invasion and pulmonary fibrosis

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