Infra-red and Raman spectroscopic studies of enzyme structure and function

2015) 'The anti-biofouling properties of thin-lm composite nanoltration membranes grafted with biogenic silver nanoparticles. ', Desalination., Further information on publisher's website: Use policyThe full-text may be used and/or reproduced, and given to third parties in any format or medium, without prior permission or charge, for personal research or study, educational, or not-for-prot purposes provided that:• a full bibliographic reference is made to the original source • a link is made to the metadata record in DRO • the full-text is not changed in any way The full-text must not be sold in any format or medium without the formal permission of the copyright holders.Please consult the full DRO policy for further details. ABSTRACT:Biofouling is still one of the most challenging issues of nanofiltration.One of the practical strategies to reduce biofouling is to develop novel anti-biofouling membranes. Herein, biogenic silver nanoparticles (BioAg 0 -6) with the averaged diameter of only 6nm were firstly grafted on the surface of polyamide NF membrane.The effect of grafted BioAg 0 -6 on the performance of thin-film composite (TFC) NF membranes was systematically investigated with a comparison to the grafted chemical AgNPs. BioAg 0 -6 grafted membrane (TFC-S-BioAg) increased the hydrophilicity of the TFC membrane and water permeability, while maintaining the relatively high salt rejection. The result of silver leaching experiment indicated that the grafted BioAg 0 -6 had a better stability on the membranes, the ratio of remained silver in the TFC-S-BioAg membrane was 95%, after soaked in pure water for 50 days. After 4 months immersion, the rejection of TFC-S-BioAg membrane remained more than 90% of initial rejection. The results of disk diffusion test revealed that both of TFC-S-BioAg membrane and TFC-S-ChemAg membrane showed effective anti-bacterial ability to inhibit P.aeruginosa and E.coli growth, the TFC-S-BioAg membrane showed more excellent and longer lasting antibacterial property. Therefore, BioAg 0 -6 grafed TFC membranes could be potential as an effective strategy to decrease biofouling in nanofiltration process.

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“…This may because of the low sensitivity of thiol group in FT-IR due to the high polarizability of sulfur 29 .…”

Section: Resultsmentioning

confidence: 99%

The anti-biofouling properties of thin-film composite nanofiltration membranes grafted with biogenic silver nanoparticles

Liu

Fang

et al. 2015

Desalination

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“…Amide I, this is primarily characterized by carbonyl stretch at 1630–1670 cm −1 and intense. Thus, α‐helix is characterized by 1645–1660 cm −1 , β‐sheet at 1665–1680 cm −1 and random coil at 1660–1670 cm −1 are intense in both IR and Raman . However, the presence of amide II at frequency 1530–1550 cm −1 and amide III (1230 cm −1 ), Raman‐active occur at 1230–1300 cm −1 .…”

Section: Discussionmentioning

confidence: 99%

Thermostable lipase fromGeobacillussp. Iso5: Bioseparation, characterization and native structural studies

Mahadevan

Neelagund

2013

J. Basic Microbiol.

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The extracellular thermoalkaline lipase from Geobacillus sp. Iso5 was purified to homogeneity by ultrafiltration, 6% cross-linked agarose and Phenyl spehrose HIC column chromatography. The final purified lipase resulted in 8.7-fold with 6.2% yield. The relative molecular weight of the enzyme was determined to be a monomer of 47 kDa by SDS-PAGE and MALDI-TOF MS/MS spectroscopy. The purified enzyme exhibit optimum activity at 70 °C and pH 8.0. The enzyme retained above 90% activity at temperatures of 70 °C and about 35% activity at 85 °C for 2 h. However, the stability of the enzyme decreased at the temperature over 90 °C. The enzyme activity was promoted in the presence of Ca(2+) and Mg(2+) and strongly inhibited by HgCl2 , PMSF, DTT, K(+) , Co(2+) , and Zn (2+) . EDTA did not affect the enzyme activity. The secondary structure of purified lipase contains 36% α-helix and 64% β-sheet which was determined by Circular dichromism, FTIR, and Raman Spectroscopy.

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“…Important clues to the polypeptide structure can be estimated from in-plane vibrational modes of the amide groups [Wharton, 1986]. Amide 1 which is primarily characterised by the carbonyl stretch normally has an intense absorption at 1,630-1,680 cm-1 depending on 2° structure.…”

Section: Spectrum O F Sulphated Glycoproteinmentioning

confidence: 99%

“…Amide 1 which is primarily characterised by the carbonyl stretch normally has an intense absorption at 1,630-1,680 cm-1 depending on 2° structure. Previ ously quoted evidence on polypeptides [Wharton, 1986] indicates that a-helix predominates at 1,645-1,660 cm '1, (3-sheet at 1,665-1,680 and random coil at 1,660-1,670 cm '1. Based on the intense absorption present in the sulphated glycoprotein at a maximum o f 1,660 cm' 1 it seems likely that both random coil and a-helix contribute to the polypeptide structure, al though it is difficult to separate the contribution of (3-sheet and random coil due to the close proximity of their absorption maxima.…”

Section: Spectrum O F Sulphated Glycoproteinmentioning

confidence: 99%

“…Infrared spec troscopy has previously been used to study the inter action of acidic glycosaminoglycans with hydroxyap atite [Embery and Rolla, 1980], the different types of oral in vivo films formed on different surfaces [Baier and Glantz, 1978] and to investigate the degree o f sub stitution of hydroxyl by carbonate ions in human dental enamel [Elliott et al, 1985]. Recently FT IR has been used to investigate the secondary structure of two phosphoproteins [Renugopalakrishnan et al, 1986] and also certain mechanisms of enzyme struc ture and function [Wharton, 1986]. …”

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confidence: 99%

See 1 more Smart Citation

Structural Probe Analysis on the Attachment of Salivary Glycoproteins to Hydroxyapatite Using Fourier-Transformed Infrared Spectroscopy

Embery

Green²,

Rolla³

1989

Caries Res

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The mechanisms of interaction between a salivary glycoprotein and hydroxyapatite has been studied using Fourier-transformed infrared spectroscopy. The spectra of the reacted mixtures showed evidence of change at 1,230–1,250 cm^-1 due to the S =·stretching vibrations of the ester sulphate groups and at 775 and 828 cm^––1 attributed to sulphate groups in the 6-position of N-acetylgalactosamine. Evidence of amide change in the regions of 1,670 and 1,550 cm^––1 is also apparent due to involvement of the polypeptide region. It is proposed that the exposed parts of the protein core are involved in the attachment of the glycoprotein to hydroxyapatite with the main interactive residues being the sulphate groups present on the carbohydrate apo-protein moiety. The results are considered of value in relation to mechanisms involved in the coating of exposed apatite surfaces during the formation of acquired enamel pellicle.

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Infra-red and Raman spectroscopic studies of enzyme structure and function

Cited by 36 publications

References 89 publications

The anti-biofouling properties of thin-film composite nanofiltration membranes grafted with biogenic silver nanoparticles

The anti-biofouling properties of thin-film composite nanofiltration membranes grafted with biogenic silver nanoparticles

Thermostable lipase fromGeobacillussp. Iso5: Bioseparation, characterization and native structural studies

Structural Probe Analysis on the Attachment of Salivary Glycoproteins to Hydroxyapatite Using Fourier-Transformed Infrared Spectroscopy

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