Influenza virus hemagglutinin HA-2 N-terminal fusogenic peptides augment gene transfer by transferrin-polylysine-DNA complexes: toward a synthetic virus-like gene-transfer vehicle.

Wagner, Ernst; Plank, Christian; Zatloukal, Kurt; Cotten, Matthew; Birnstiel, Max L.

doi:10.1073/pnas.89.17.7934

Cited by 641 publications

(349 citation statements)

References 32 publications

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“…The latter has previously been shown to facilitate DNA delivery by poly-l-lysine based molecular conjugates. [11][12][13] A fusigenic liposome with a viral fusion protein for gene delivery was recently constructed by fusion of UVinactivated Sendai virus with DNA/cationic lipid vesicles. 32 However, the application of an inactivated virus raises safety concerns regarding application for gene therapy.…”

Section: Nmol) Were Mixed With Increasing Amounts Of Virosomes Contaimentioning

confidence: 99%

“…9,4,10 For the latter the membrane-destabilizing properties of the fusion peptide from hemagglutinin was employed. [11][12][13] Hemagglutinin (HA), the membrane fusion protein of influenza virus mediating viral cell entry, is composed of two membrane subunits, HA1 and HA2. [14][15][16] The virus binds to cells through interaction of the HA1 subunits with sialylated lipids (gangliosides) or proteins in the membrane of target cells.…”

Section: Introductionmentioning

confidence: 99%

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Gene transfer mediated by fusion protein hemagglutinin reconstituted in cationic lipid vesicles

et al. 1999

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Hemagglutinin, the membrane fusion protein of influenza erythrocyte ghosts. Efficient cell transfection of BHK-21 virus, is known to mediate a low-pH-dependent fusion cells was observed with virosomes containing 30 mol% reaction between the viral envelope and the limiting mem-DODAC and plasmid encoding for ␤-galactosidase (pCMV brane of the endosomal cell compartment following cellular ␤-gal) associated to their surface. The transfection activity uptake of the virus particles by receptor-mediated endoobserved was dependent on the functional activity of hemcytosis. Here we exploited this activity of hemagglutinin to agglutinin. Contrary to DNA/cationic lipid complexes the achieve efficient gene delivery to cultured cells. Hemagglutransfection was not dependent on the cationic lipid to DNA tinin was reconstituted in the presence of the monocationic charge ratio. Importantly, transfection of BHK-21 cells with lipid dioleoyldimethylammonium chloride (DODAC) to perpCMV ␤-gal by DODAC-containing virosomes did not show mit plasmid binding to the virosome surface. Virosomes any significant signs of cytotoxicity that is commonly with 30 mol% DODAC exhibited a distinct binding capacity observed with DNA/cationic lipid complexes. Together with for plasmid without causing aggregation. The virosome the high levels of expression of the transgene this highfusion activity was not affected by the cationic lipid DODAC lights the potential of DODAC-containing virosomes as a as demonstrated by low-pH-dependent lipid mixing with novel approach in nonviral gene transfer.

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Section: Nmol) Were Mixed With Increasing Amounts Of Virosomes Contaimentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Gene transfer mediated by fusion protein hemagglutinin reconstituted in cationic lipid vesicles

et al. 1999

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“…1,2 In addition, the simple conjugation of a targeting moiety to nonviral gene carrier can facilitate tissue-targeting gene delivery. [3][4][5][6][7][8][9][10][11][12][13] To use it for cardiovascular disease gene therapy, we have developed a new gene carrier system, TerplexD-NA. [14][15][16][17][18] TerplexDNA is composed of plasmid DNA (pDNA), stearyl-poly-L-lysine (stearyl-PLL), and lowdensity lipoprotein (LDL).…”

Section: Introductionmentioning

confidence: 99%

Water-soluble lipopolymer as an efficient carrier for gene delivery to myocardium

et al. 2003

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Water-soluble lipopolymer (WSLP), which consisted of polyethylenimine (PEI, 1800 Da) and cholesterol, was characterized as a gene carrier to smooth muscle cells and myocardium. Acid-base titration showed that WSLP had a proton-buffering effect. The size of WSLP/plasmid DNA (pDNA) complex was around 70 nm. WSLP/pDNA complex was transfected to A7R5 cells, a smooth muscle cell line. WSLP showed the highest transfection at a 40/1 N/P ratio. WSLP has higher transfection efficiency than PEI (1800 and 25 000 Da), SuperFect, and lipofectamine. In addition, WSLP has less cytotoxicity than PEI (25 000 Da), SuperFect, and lipofectamine. Since WSLP has cholesterol moiety, it may utilize cellular cholesterol uptake pathway, in which lowdensity lipoprotein (LDL) is involved. An inhibition study with free cholesterol or low-density lipoprotein (LDL) showed that transfection was inhibited by cholesterol or LDL, suggesting that WSLP/pDNA complex is transfected to the cells through the cholesterol uptake pathway. To evaluate the transfection efficiency to myocardium, WSLP/pDNA complex was injected into the rabbit myocardium. WSLP showed higher transfection than PEI and naked pDNA. WSLP expressed the transgene for more than 2 weeks. In conclusion, WSLP is an efficient carrier for local gene transfection to myocardium, and useful in in vivo gene therapy.

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“…(Ren et al, 2004), recombinant cross-packaging of adeno-associated virus (Aikawa et al, 2002;Li et al, 2003), and lentivirus (Sakoda et al, 2003) have been the choice for gene delivery to cardiomyocytes because of their high efficiency, but they are immunogenic and may raise risk to patients (Mulligan, 1993). Compared with viral vectors, synthetic polymers have the advantages of no specific immunogenecity, low risk, and ease of large-scale production, and thus have been considered as a potential alternative to viral vectors (Nishikawa and Huang, 2001;Templeton and Lasic, 1999;Wagner et al, 1992). However, so far there is little study on polymer mediated gene delivery to cardiomyocytes.…”

Section: Introductionmentioning

confidence: 99%

Biodegradable cationic polyester as an efficient carrier for gene delivery to neonatal cardiomyocytes

et al. 2006

Biotech & Bioengineering

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Viral-mediated gene delivery has been explored for the treatment and protection of cardiomyocytes, but so far there is only one report using cationic polymer for gene delivery to cardiomyocytes in spite of many advantages of polymer-mediated gene delivery. In this study, a cationic poly(b-amino ester) (PDMA) with a degradable backbone and cleavable side chains was synthesized by Michael addition reaction. The toxicity of PDMA to neonatal mouse cardiomyocytes (NMCMs) was significantly lower than that of polyethyleneimine (PEI). PDMA formed stable polyplexes with pEGFP. The dissociation of the polyplexes could be triggered by PDMA degradation, and the dissociation time was tunable via the polymer/pEGFP ratio. In vitro transfection showed that PDMA was an effective and low toxic gene delivery carrier for NMCMs. The PDMA/pEGFP polyplexes transfected EGFP gene to NMCMs with about 28% efficiency and caused little death. In contrast, a significant portion of cardiomyocytes cultured with PEI/pEGFP died.

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Influenza virus hemagglutinin HA-2 N-terminal fusogenic peptides augment gene transfer by transferrin-polylysine-DNA complexes: toward a synthetic virus-like gene-transfer vehicle.

Cited by 641 publications

References 32 publications

Gene transfer mediated by fusion protein hemagglutinin reconstituted in cationic lipid vesicles

Gene transfer mediated by fusion protein hemagglutinin reconstituted in cationic lipid vesicles

Water-soluble lipopolymer as an efficient carrier for gene delivery to myocardium

Biodegradable cationic polyester as an efficient carrier for gene delivery to neonatal cardiomyocytes

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