Influenza surveillance and laboratory diagnosis

Zambon, Maria

doi:10.1002/9781118636817.ch15

Cited by 6 publications

(9 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Molecular diagnosis is one of the most dynamically developing fields of biology and medicine. The main differences between conventional and molecular diagnostics include shorter duration as well as increased specificity and sensitivity; therefore, molecular diagnostics has become increasingly used in different fields of science, including biotechnology and medicine (5,7,9,11) . For many years, various molecular biology techniques have aroused the greatest interest among the methods of virological diagnosis.…”

Section: Virological Diagnosismentioning

confidence: 99%

“…The two-step RT-PCR seems to show higher specificity and efficiency compared with its one-step equivalent. However, one-step PCR is more rapid, reduces the risk of sample contamination and increases the reproducibility of results (7,11,12) . Conventional PCR is increasingly replaced by RT-PCR.…”

Section: Conventional Pcrmentioning

confidence: 99%

“…Depending on the type of test, the results are obtained within several hours (9,10) . This is of key importance for the initiation of treatment, which should be implemented 36-48 hours after the onset of first symptoms and involve the use of new generation anti-influenza drugs (5,8,(11)(12)(13)(14)(15) . Influenza is not a pathognomonic disease.…”

mentioning

confidence: 99%

“…small children, chronically ill patients, patients on immunosuppressive therapy, the elderly and high-risk patients, who may experience severe illness and complications compared to potentially healthy individuals (high risk of complications) (1,4,8,12) . For this reason, it is important to rapidly identify the aetiological factor to decide on further management, considering the possible use of new generation anti-influenza drugs -neuraminidase inhibitors (4,7,8,11,13) . Laboratory diagnosis involves: • confirmation of influenza virus antigen in the material collected from the patient; • serological confirmation of influenza virus infection based on increased serum antibody levels.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Laboratory diagnosis of influenza

Byambasuren¹,

Brydak

2018

PEDIATR MED RODZ

View full text Add to dashboard Cite

Influenza has always been and still is the cause of considerable morbidity and, consequently, frequent multiorgan complications, often irreversible and even fatal. It is an acute infectious disease caused by type A, B and C viruses, members of the family Orthomyxoviridae. Infections caused by the influenza virus are reported in every epidemic season. Influenza infections should be considered not only in the aspect of health, but also in the quantifiable, measurable economic aspect. For many years, influenza has been one of the basic priorities of public health. Virological and epidemiological surveillance of influenza, which is implemented in each epidemiological season, is one of the key elements of public health. Virological surveillance involves laboratory confirmation of infection, while epidemiological surveillance involves monitoring of actual and suspected cases of influenza. Laboratory diagnosis is performed to confirm influenza virus antigen in the material collected from the patient, isolate the virus and confirm viral infection based on increased serum antibody levels. Isolating influenza viruses that circulate in a given epidemiological season is necessary to prepare a vaccine against influenza. An early and correct virological diagnosis of respiratory infection, with particular reference to influenza, is currently of great importance in terms of both medical and economic aspects. The paper discusses influenza diagnostic methods currently used in Poland to help physicians in deciding whether laboratory confirmation of diagnosis is justified in the aspect of possible treatment to avoid influenza-induced multiple organ complications.

show abstract

Section: Virological Diagnosismentioning

confidence: 99%

Section: Conventional Pcrmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Laboratory diagnosis of influenza

Byambasuren¹,

Brydak

2018

PEDIATR MED RODZ

View full text Add to dashboard Cite

show abstract

“…RIDT specificity, on the other hand, has been very good ranging from 85% to 100%, thus, they are good tests for "ruling-in" rather than "ruling out" influenza infection, especially when influenza activity is high in the community [22]. Two recent FDA-cleared assay systems that rely on instrument optics to determine an objective result, as opposed to a subjective read by the operator, hope to improve performance of RIDTs [26].…”

Section: Diagnosismentioning

confidence: 99%