Influenza A virus molecularly imprinted polymers and their application in virus sub-type classification

Wangchareansak, Thipvaree; Thitithanyanont, Arunee; Chuakheaw, Daungmanee; Gleeson, M. Paul; Lieberzeit, Peter A.; Sangma, Chak

doi:10.1039/c3tb00027c

Cited by 79 publications

(65 citation statements)

References 67 publications

(69 reference statements)

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“…Using five virus strains (H5N1, H5N3, H1N1, H1N3, and H6N1) as the template, Wangchareansak et al synthesized five unique MIPs. Combining with QCM (Figure B), the relative selectivity of these five influenza A subtypes was evaluated . Studies have shown that each virus subtype MIPs showed significantly different characteristics, corresponding to a unique biomolecular fingerprint, which could quickly and cheaply distinguish and screen virus subtypes.…”

Section: Biological Molecular Imprinting With Different Scalementioning

confidence: 99%

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Molecularly Imprinted Materials for Selective Biological Recognition

Zhang

et al. 2019

Macromol. Rapid Commun.

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Molecular imprinting is an approach of generating imprinting cavities in polymer structures that are compatible with the target molecules. The cavities have memory for shape and chemical recognition, similar to the recognition mechanism of antigen–antibody in organisms. Their structures are also called biomimetic receptors or synthetic receptors. Owing to the excellent selectivity and unique structural predictability of molecularly imprinted materials (MIMs), practical MIMs have become a rapidly evolving research area providing key factors for understanding separation, recognition, and regenerative properties toward biological small molecules to biomacromolecules, even cell and microorganism. In this review, the characteristics, morphologies, and applicability of currently popular carrier materials for molecular imprinting, especially the fundamental role of hydrogels, porous materials, hierarchical nanoparticles, and 2D materials in the separation and recognition of biological templates are discussed. Moreover, through a series of case studies, emphasis is given on introducing imprinting strategies for biological templates with different molecular scales. In particular, the differences and connections between small molecular imprinting (bulk imprinting, “dummy” template imprinting, etc.), large molecular imprinting (surface imprinting, interfacial imprinting, etc.), and cell imprinting strategies are demonstrated in detail. Finally, future research directions are provided.

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Section: Biological Molecular Imprinting With Different Scalementioning

confidence: 99%

“…Copyright 2014, Wiley‐VCH; B) outline of the imprinting process used. Reproduced with permission . Copyright 2013, Royal Society of Chemistry; C) left: schematic of fabrication and materials used in the synthesis.…”

Section: Biological Molecular Imprinting With Different Scalementioning

confidence: 99%

Molecularly Imprinted Materials for Selective Biological Recognition

Zhang

et al. 2019

Macromol. Rapid Commun.

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“…MIP‐based sensors can identify structures ranging from small to large molecules, proteins, or even whole microorganisms such as bacteria or viruses . In case of virus MIP biosensors, the main applications are virus detection, classification, or virus binding assays . However, applications of virus imprinting beyond these types have not been reported.…”

Section: Figurementioning

confidence: 99%

Small‐Molecule Dengue Virus Co‐imprinting and Its Application as an Electrochemical Sensor

et al. 2017

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Polymers can be synthesized to recognize small molecules. This is achieved by introducing the target molecule during monomer self‐assembly, where they can be incorporated during cross‐linking polymerization. Following additional pre‐processing, the material obtained can then be applied as a sensing layer for these molecules in many applications. The sensitivity of the polymers depends on the “active sites” imprinted on the surface. Increasing the number of active sites on the polymers surface can be achieved by using nanoparticles as a platform to support and concentrate the molecules for imprinting. In this work, we report the first use of dengue virus as a supporting nanoparticle to make for a more effective polymer composite sensor for the detection of bisphenol A (BPA), which is an environmental contaminant. The dengue virus has a nanoparticle size of around 100 nm and its surface provides regions where lipids and hydrophobic compounds can bind, making it an ideal support. The mixing of BPA with dengue prior to monomer self‐assembly led to imprinted polymer surfaces with much higher density BPA binding sites and a limit of detection of 0.1 pm. We demonstrate that a BPA–dengue co‐imprinting polymer composite sensor shows a very high sensitivity for BPA, but with lower production costs and technical requirements than other comparable methods.

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“…The sensing measurements revealed that each type of imprinted surface offered the highest sensor response to its templated strain virus as shown in Figure 3B, thus proving excellent selectivity. Similarly, the surface imprinted polymers designed by soft-lithography procedure are successfully employed in QCM-based viral diagnostics for influenza A virus sub-types, i.e., H5N1, H5N3, H1N1, H1N3, and H6N1 [67,78]. The fabricated sensors demonstrated considerable selectivity for screening of influenza A virus sub-types as each sub-type was best recognized by its own imprinted surface.…”

Section: Synthetic Antibodies: Molecularly Imprinted Polymers For Qcmmentioning

confidence: 99%

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

et al. 2017

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Viruses are pathogenic microorganisms that can inhabit and replicate in human bodies causing a number of widespread infectious diseases such as influenza, gastroenteritis, hepatitis, meningitis, pneumonia, acquired immune deficiency syndrome (AIDS) etc. A majority of these viral diseases are contagious and can spread from infected to healthy human beings. The most important step in the treatment of these contagious diseases and to prevent their unwanted spread is to timely detect the disease-causing viruses. Gravimetric viral diagnostics based on quartz crystal microbalance (QCM) transducers and natural or synthetic receptors are miniaturized sensing platforms that can selectively recognize and quantify harmful virus species. Herein, a review of the label-free QCM virus sensors for clinical diagnostics and point of care (POC) applications is presented with major emphasis on the nature and performance of different receptors ranging from the natural or synthetic antibodies to selective macromolecular materials such as DNA and aptamers. A performance comparison of different receptors is provided and their limitations are discussed.

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Influenza A virus molecularly imprinted polymers and their application in virus sub-type classification

Cited by 79 publications

References 67 publications

Molecularly Imprinted Materials for Selective Biological Recognition

Molecularly Imprinted Materials for Selective Biological Recognition

Small‐Molecule Dengue Virus Co‐imprinting and Its Application as an Electrochemical Sensor

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

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