Influenza A virus haemagglutinin polymorphism: pleiotropic antigenic variants of A/Shanghai/11/87 (H3N2) virus selected as high yield reassortants

Kilbourne, Edwin D.; Johansson, Bert E.; Moran, Timothy H.; Wu, Shengbiao; Pokorny, Barbara A.; Xu, Xinhua; Cox, Nancy J.

doi:10.1099/0022-1317-74-7-1311

Cited by 14 publications

(18 citation statements)

References 30 publications

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“…However, the majority of these studies reported using viruses isolated between 1986 and 1990 (19,24,26,30,31,44,56,83), while one studied a single 1997 virus (43) and two studies looked at a A/Fujian/2002-like virus (38,78). Our studies with 9 pairs of viruses isolated in 2006 and 2007 identified substitutions at 8 of these 16 previously reported positions, and all were close to the RBS (Fig.…”

Section: Discussionmentioning

confidence: 78%

“…First, the majority of the variation occurred at only eight amino acid positions, all of which are vicinal to the receptor binding site in the HA1. Second, most changes involved the 16 amino acids previously reported to vary in relation to isolation in eggs (19,24,26,30,31,38,43,44,56,78,83).…”

Section: Discussionmentioning

confidence: 99%

“…Glycans that bound to the antibody-only control slide (see Table 1 for all virus abbreviations) (16,27,45). Sequence analysis of the HAs from the paired isolates shows that the majority of amino acid changes in the egg-grown virus relative to the sequence of the cell-grown virus occur at 8 of the 16 positions that have previously been reported to be egg isolation-associated sites in older viruses, i.e., positions 138,156,168,183,186,193,194, and 219 (Table 2) (19,24,26,30,31,38,43,44,78,83,84). This study also revealed changes at an additional seven new positions (positions 86,142,190,195,198, 223 and 225) that have not previously been associated with H3N2 virus growth in eggs, although two of these amino acid substitutions (at positions 190 and 195) were found only as mixed populations ( Fig.…”

Section: Viruses and Cellsmentioning

confidence: 99%

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Receptor Specificity of Influenza A H3N2 Viruses Isolated in Mammalian Cells and Embryonated Chicken Eggs

Stevens

Chen

Carney

et al. 2010

J Virol

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Isolation of human subtype H3N2 influenza viruses in embryonated chicken eggs yields viruses with amino acid substitutions in the hemagglutinin (HA) that often affect binding to sialic acid receptors. We used a glycan array approach to analyze the repertoire of sialylated glycans recognized by viruses from the same clinical specimen isolated in eggs or cell cultures. The binding profiles of whole virions to 85 sialoglycans on the microarray allowed the categorization of cell isolates into two groups. Group 1 cell isolates displayed binding to a restricted set of ␣2-6 and ␣2-3 sialoglycans, whereas group 2 cell isolates revealed receptor specificity broader than that of their egg counterparts. Egg isolates from group 1 showed binding specificities similar to those of cell isolates, whereas group 2 egg isolates showed a significantly reduced binding to ␣2-6-and ␣2-3-type receptors but retained substantial binding to specific O-and N-linked ␣2-3 glycans, including ␣2-3GalNAc and fucosylated ␣2-3 glycans (including sialyl Lewis x), both of which may be important receptors for H3N2 virus replication in eggs. These results revealed an unexpected diversity in receptor binding specificities among recent H3N2 viruses, with distinct patterns of amino acid substitution in the HA occurring upon isolation and/or propagation in eggs. These findings also suggest that clinical specimens containing viruses with group 1-like receptor binding profiles would be less prone to undergoing receptor binding or antigenic changes upon isolation in eggs. Screening cell isolates for appropriate receptor binding properties might help focus efforts to isolate the most suitable viruses in eggs for production of antigenically well-matched influenza vaccines.

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Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Viruses and Cellsmentioning

confidence: 99%

See 1 more Smart Citation

Receptor Specificity of Influenza A H3N2 Viruses Isolated in Mammalian Cells and Embryonated Chicken Eggs

Stevens

Chen

Carney

et al. 2010

J Virol

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“…While both HA and NA are highly susceptible to antigenic drift, Kilbourne et al 22 determined that, over a thirty year period, HA evolved more rapidly than did NA. NP and M1 drifted even more slowly than did the surface glycoproteins.…”

Section: Influenza Antigensmentioning

confidence: 99%

“…25 Nonetheless, mutations occur sequentially during the spread of a virus, such that a change in a single epitope may engender some selective advantage. 22 Significant change from an epidemiological viewpoint generally requires changes to accumulate in two or more sites. 26 Antigenic shift.…”

Section: Influenza Antigensmentioning

confidence: 99%

A call to cellular & humoral arms: Enlisting cognate T cell help to develop broad-spectrum vaccines against influenza A

McMurry¹,

Johansson²,

Groot³

2008

Human Vaccines

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HA1 domain of influenza A (H3N2) viruses in Finland in 1989–1995: evolution, egg-adaptation and relationship to vaccine strains

Pyhälä

Ikonen

Haanpää

et al. 1996

Archives of Virology

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The HA1 gene sequences of 22 MDCK cell-derived influenza A (H3N2) strains, ten of their egg-derived counterparts and three vaccine strains were determined. Antigenic and sequence differences between the epidemic and vaccine strains were recorded, most striking in 1992/93; a minority of the amino acid differences in 1989-95 was involved in egg-adaptation. Changes in the assortment of amino acid substitutions produced during egg-adaptation of field strains may account for the difficulty encountered in isolating these viruses in embryonated eggs. Six revertant amino acids, characteristic of field strains prevalent in 1969-71 were recorded in 1994/95. Their genome sequence was interpreted to have been maintained over the interval years among low abundant sequences of the viral quasispecies. Potential changes of carbohydrate moieties were recorded in two glycosylation sites, suggesting that oligosaccharides at these sites are not necessarily advantageous for the H3N2 subtype virus currently.

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Influenza A virus haemagglutinin polymorphism: pleiotropic antigenic variants of A/Shanghai/11/87 (H3N2) virus selected as high yield reassortants

Cited by 14 publications

References 30 publications

Receptor Specificity of Influenza A H3N2 Viruses Isolated in Mammalian Cells and Embryonated Chicken Eggs

Receptor Specificity of Influenza A H3N2 Viruses Isolated in Mammalian Cells and Embryonated Chicken Eggs

A call to cellular & humoral arms: Enlisting cognate T cell help to develop broad-spectrum vaccines against influenza A

HA1 domain of influenza A (H3N2) viruses in Finland in 1989–1995: evolution, egg-adaptation and relationship to vaccine strains

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