Influenza A pathway analysis of highly pathogenic avian influenza virus (H5N1) infection in genetically disparate Ri chicken lines

Lee, Jiae; Hong, Yeojin; Vu, Thi Hao; Lee, Sooyeon; Heo, Jubi; Truong, Anh Duc; Lillehoj, Hyun S.

doi:10.1016/j.vetimm.2022.110404

Cited by 8 publications

(10 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In previous study, gene expression patterns on trachea from H5N1-infected Ri chicken were analyzed by RNA-seq [ 13 ]. Few DE miRNAs in infection samples of resistant line and susceptible line were showed negative correlation with DE genes from RNA-seq result that conducted with same tracheal RNA samples ( Supplementary Table 4 ).…”

Section: Resultsmentioning

confidence: 99%

“…Several studies have performed small RNA sequencing (RNA-seq) in lung and immune organs such as the thymus, bursa of Fabricius, and spleen of H5N1-infected chickens [ 10 11 12 ]. Moreover, we previously conducted RNA-seq analyses of lung and trachea tissues of H5N1-infected Ri chicken [ 13 14 ]. In this study, we analyzed the expression of miRNAs and their target genes in tracheae from two genetically distinct Ri chicken lines infected with HPAIV H5N1, after which bioinformatic analyses were conducted to identify differentially expressed (DE) miRNAs and their target genes.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Analysis of miRNA expression in the trachea of Ri chicken infected with the highly pathogenic avian influenza H5N1 virus

Kang,

Vu,

Heo

et al. 2023

J Vet Sci

Self Cite

View full text Add to dashboard Cite

Background Highly pathogenic avian influenza virus (HPAIV) is considered a global threat to both human health and the poultry industry. MicroRNAs (miRNA) can modulate the immune system by affecting gene expression patterns in HPAIV-infected chickens. Objectives To gain further insights into the role of miRNAs in immune responses against H5N1 infection, as well as the development of strategies for breeding disease-resistant chickens, we characterized miRNA expression patterns in tracheal tissues from H5N1-infected Ri chickens. Methods miRNAs expression was analyzed from two H5N1-infected Ri chicken lines using small RNA sequencing. The target genes of differentially expressed (DE) miRNAs were predicted using miRDB. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis were then conducted. Furthermore, using quantitative real-time polymerase chain reaction, we validated the expression levels of DE miRNAs (miR-22-3p, miR-146b-3p, miR-27b-3p, miR-128-3p, miR-2188-5p, miR-451, miR-205a, miR-203a, miR-21-3p, and miR-200a-3p) from all comparisons and their immune-related target genes. Results A total of 53 miRNAs were significantly expressed in the infection samples of the resistant compared to the susceptible line. Network analyses between the DE miRNAs and target genes revealed that DE miRNAs may regulate the expression of target genes involved in the transforming growth factor-beta, mitogen-activated protein kinase, and Toll-like receptor signaling pathways, all of which are related to influenza A virus progression. Conclusions Collectively, our results provided novel insights into the miRNA expression patterns of tracheal tissues from H5N1-infected Ri chickens. More importantly, our findings offer insights into the relationship between miRNA and immune-related target genes and the role of miRNA in HPAIV infections in chickens.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysis of miRNA expression in the trachea of Ri chicken infected with the highly pathogenic avian influenza H5N1 virus

Kang,

Vu,

Heo

et al. 2023

J Vet Sci

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, they could not rule out the possible influence of other polymorphisms within the Mx gene in the in vivo antiviral activity. In a recent study Lee et al [ 52 ] showed that MX1 , STAT1 , IRF7 , and TLR3 genes were upregulated in resistant Ri chicken lines in comparison to susceptible lines. The immune response to infection in HPAIV-resistant Ri chicken lines was different than in HPAIV-susceptible Ri chicken lines.…”

Section: Discussionmentioning

confidence: 99%

Myxovirus resistance (Mx) Gene Diversity in Avian Influenza Virus Infections

Jahangir¹,

Rahman

Halder

et al. 2022

Biomedicines

View full text Add to dashboard Cite

Avian influenza viruses (AIVs) pose threats to animal and human health. Outbreaks from the highly pathogenic avian influenza virus (HPAIV) in indigenous chickens in Bangladesh are infrequent. This could be attributed to the Myxovirus resistance (Mx) gene. To determine the impact of Mx gene diversity on AIV infections in chicken, we assessed the Mx genes, AIVs, and anti-AIV antibodies. DNA from blood cells, serum, and cloacal swab samples was isolated from non-vaccinated indigenous chickens and vaccinated commercial chickens. Possible relationships were assessed using the general linear model (GLM) procedure. Three genotypes of the Mx gene were detected (the resistant AA type, the sensitive GG type, and the heterozygous AG type). The AA genotype (0.48) was more prevalent than the GG (0.19) and the AG (0.33) genotypes. The AA genotype was more prevalent in indigenous than in commercial chickens. A total of 17 hemagglutinating viruses were isolated from the 512 swab samples. AIVs were detected in two samples (2/512; 0.39%) and subtyped as H1N1, whereas Newcastle disease virus (NDV) was detected in the remaining samples. The viral infections did not lead to apparent symptoms. Anti-AIV antibodies were detected in 44.92% of the samples with levels ranging from 27.37% to 67.65% in indigenous chickens and from 26% to 87.5% in commercial chickens. The anti-AIV antibody was detected in 40.16%, 65.98%, and 39.77% of chickens with resistant, sensitive, and heterozygous genotypes, respectively. The genotypes showed significant association (p < 0.001) with the anti-AIV antibodies. The low AIV isolation rates and high antibody prevalence rates could indicate seroconversion resulting from exposure to the virus as it circulates. Results indicate that the resistant genotype of the Mx gene might not offer anti-AIV protection for chickens.

show abstract

“…Therefore, chickens with the Mx (A)/ BF2 (B21) genotype were selected as HPAIV-resistant Ri chickens, and chickens with the Mx (G)/ BF2 (B13) genotype were selected as HPAIV-susceptible Ri chickens. Twenty chickens from each line were randomly assigned to control and infection groups [ 12 ]. The A/duck/Vietnam/QB1207/2012 (H5N1) virus strain was harvested from the allantoic fluid of 10 4 egg infectious dose (EID50).…”

Section: Methodsmentioning

confidence: 99%

HPAI-resistant Ri chickens exhibit elevated antiviral immune-related gene expression

Heo

Hong

et al. 2023

J Vet Sci

Self Cite

View full text Add to dashboard Cite

Background Highly pathogenic avian influenza viruses (HPAIVs) is an extremely contagious and high mortality rates in chickens resulting in substantial economic impact on the poultry sector. Therefore, it is necessary to elucidate the pathogenic mechanism of HPAIV for infection control. Objective Gene set enrichment analysis (GSEA) can effectively avoid the limitations of subjective screening for differential gene expression. Therefore, we performed GSEA to compare HPAI-infected resistant and susceptible Ri chicken lines. Methods The Ri chickens Mx (A)/ BF2 (B21) were chosen as resistant, and the chickens Mx (G)/ BF2 (B13) were selected as susceptible by genotyping the Mx and BF2 genes. The tracheal tissues of HPAIV H5N1 infected chickens were collected for RNA sequencing followed by GSEA analysis to define gene subsets to elucidate the sequencing results. Results We identified four differentially expressed pathways, which were immune-related pathways with a total of 78 genes. The expression levels of cytokines ( IL-1β , IL-6 , IL-12 ), chemokines ( CCL4 and CCL5 ), type interferons and their receptors ( IFN-β , IFNAR1 , IFNAR2 , and IFNGR1 ), Jak-STAT signaling pathway genes ( STAT1 , STAT2 , and JAK1 ), MHC class I and II and their co-stimulatory molecules ( CD80 , CD86 , CD40 , DMB2 , BLB2 , and B2M ), and interferon stimulated genes ( EIF2AK2 and EIF2AK1 ) in resistant chickens were higher than those in susceptible chickens. Conclusions Resistant Ri chickens exhibit a stronger antiviral response to HPAIV H5N1 compared with susceptible chickens. Our findings provide insights into the immune responses of genetically disparate chickens against HPAIV.

show abstract

Influenza A pathway analysis of highly pathogenic avian influenza virus (H5N1) infection in genetically disparate Ri chicken lines

Cited by 8 publications

References 56 publications

Analysis of miRNA expression in the trachea of Ri chicken infected with the highly pathogenic avian influenza H5N1 virus

Analysis of miRNA expression in the trachea of Ri chicken infected with the highly pathogenic avian influenza H5N1 virus

Myxovirus resistance (Mx) Gene Diversity in Avian Influenza Virus Infections

HPAI-resistant Ri chickens exhibit elevated antiviral immune-related gene expression

Contact Info

Product

Resources

About