Influencing factors on the preservation of lytic bacteriophage VP3

Xiao, Yue; Huang, Pin I.; Huang, Zhenzhou; Yu, Kaijiang; Yang, Song; Guo, Ning; Dai, Honghua; Jiang, Meihui; Xu, Yangsheng; Wang, Duochun; Wei, Qiang

doi:10.1016/j.bsheal.2022.06.004

Cited by 8 publications

(2 citation statements)

References 31 publications

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Section: Resultsmentioning

confidence: 88%

“…A previous detailed study on the use of solvents (DMSO/glycerol) to store a phage (VP3) showed these were not very effective, with only 50% recovery (judged by PFU counts) when stored, and hence, “not everything” can protect them. 75 In contrast, our approach allowed higher recovery rates for the E. coli phage. The exact mechanism of damage to bacteriophage due to cold exposure is difficult to pinpoint due to the challenges of studying it in isolation and hence also the mechanism of protection of the polymers.…”

Section: Resultsmentioning

confidence: 94%

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Screening of Hydrophilic Polymers Reveals Broad Activity in Protecting Phages during Cryopreservation

Marton,

Bhatt,

Sagona

et al. 2023

Biomacromolecules

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Bacteriophages have many biotechnological and therapeutic applications, but as with other biologics, cryopreservation is essential for storage and distribution. Macromolecular cryoprotectants are emerging for a range of biologics, but the chemical space for polymer-mediated phage cryopreservation has not been explored. Here we screen the cryoprotective effect of a panel of polymers against five distinct phages, showing that nearly all the tested polymers provide a benefit. Exceptions were poly(methacrylic acid) and poly(acrylic acid), which can inhibit phage-infection with bacteria, making post-thaw recovery challenging to assess. A particular benefit of a polymeric cryopreservation formulation is that the polymers do not function as carbon sources for the phage hosts (bacteria) and hence do not interfere with post-thaw measurements. This work shows that phages are amenable to protection with hydrophilic polymers and opens up new opportunities for advanced formulations for future phage therapies and to take advantage of the additional functionality brought by the polymers.

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Section: Resultsmentioning

confidence: 88%

Section: Resultsmentioning

confidence: 94%

Screening of Hydrophilic Polymers Reveals Broad Activity in Protecting Phages during Cryopreservation

Marton,

Bhatt,

Sagona

et al. 2023

Biomacromolecules

View full text Add to dashboard Cite

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The Hydrophobic Stabilization of Pseudomonas aeruginosa Bacteriophage F8 and the Influence of Modified Bacteriophage Preparation on Biofilm Degradation

Szermer-Olearnik,

Filik-Matyjaszczyk,

Ciekot

et al. 2024

Curr Microbiol

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The bacteriophage F8 belongs to the Myoviridae group of phages and is a pathogen of Pseudomonas aeruginosa. Since Pseudomonas aeruginosa is a multidrug-resistant opportunistic bacterium and can cause serious challenges for health services, studying the potential use of phages against them is a promising approach. Pseudomonas aeruginosa can be found on medical devices because bacteria can attach to surfaces and develop biofilms, which are difficult to eradicate because of their high resistance to environmental conditions and antimicrobial therapeutics. Phage therapy is becoming promising as an alternative for the treatment of antibiotic-resistant infections, but there is still a lack of standardized protocols approved by health organizations for possible use in the clinic. In our research, we focused on the potential use of 1-octanol, which was previously used by our team to develop a method for phage purification from bacterial lysate. 1-octanol is a fatty alcohol that is mostly used in the cosmetics industry, and its advantage is that it is approved by the FDA as a food additive. In this paper, we studied the protective properties of the addition of 1-octanol for storing phage liquid preparations. We demonstrated the stabilization effect of 1-octanol addition on F8 bacteriophage preparation during storage under various conditions. Interestingly, more effective biofilm reduction was observed after treatment with the purified bacteriophage and with 1-octanol addition compared to crude lysate.

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