Influencing factors and physiochemical changes of embryogenesis through in vitro isolated microspore culture in Brassica species

Dong, Yan-Qi; Gao, Yuhong; Zhao, Te; Ren, Guangqian; Li, Yanli; Guan, Bin; Jin, Rong; Gao, Fei; Tan, Xiu-Fang; Zhu, Hong-Cai; Zhang, Yuhong; Zhang, Jinxia; Dong, Peng; Yan, Ying

doi:10.1007/s11756-021-00721-0

Cited by 7 publications

(8 citation statements)

References 240 publications

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“…Одной из самых востребованных технологий для ускорения процесса получения гибридов F 1 капустных культур является производство линий дигаплоидов в культуре изолированных микроспор (Domblides et al, 2018;Djatchouk et al, 2019;Dong et al, 2021). Данная технология ускоряет получение генетически стабильных линий, позволяет сочетать различные признаки в одном генотипе и облегчает поиск редких признаков, контролируемых рецессивными аллелями генов (Ferrie, Caswell, 2011).…”

Section: Introductionunclassified

“…Наибольший успех в получении удвоенных гаплоидов через культуру микроспор достигнут у рапса (Brassica napus L.). Эффективность технологии получения дигаплоидов у других представителей рода Brassica остается по-прежнему низкой (Shmykova et al, 2015;Dong et al, 2021). Отмечается, что микроспоры генотипически различающихся образцов Brassica oleracea, при их культивировании in vitro, в меньшей степени способны к эмбриогенезу, чем таковые B. napus и B. rapa (Winarto, Teixeira da Silva, 2011;Gu et al, 2014;Shmykova et al, 2015).…”

Section: Introductionunclassified

“…У эмбриоидов, полученных в культуре микроспор у большинства генотипически различающихся образцов Brassica, наблюдается низкая способность к прорастанию, а также непрямое прорастание с образованием адвентивных побегов или вторичный эмбриогенез (Dong et al, 2021). Кроме того, удвоение числа хромосом у полученных гаплоидов спонтанно происходит лишь у части растений.…”

Section: Introductionunclassified

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Possibilities of biotechnological methods in breeding of vegetable crops at the VIR Laboratory of Breeding and Cell Technologies

Курина

Artemyeva

2023

Biotehnologiâ i selekciâ rastenij

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Basic and applied scientific research in plant cell technologies contribute to the successful development of agricultural plant breeding, which allows the creation of new forms of plants 2-4 times faster than by traditional breeding methods. To obtain inbred lines of most vegetable crops, about 5-7 cycles of self-pollination are required. As a result, the creation of a new cultivar/hybrid takes more than 10-12 years on an average. To successfully create a variety or hybrid, it is necessary to select parental pairs in the form of inbred lines. The VIR collection of vegetables and cucurbit crops includes 52,889 accessions, representatives of 29 families, 145 genera, and 610 species. The use of biotechnological methods is an important direction for accelerating the breeding of vegetable crops. Due to the relevance of introducing cell technologies into the breeding programs of the VIR Department of Genetic Resources of Vegetable and Cucurbit Crops, a Laboratory of Breeding and Cell Technologies was set up in 2022. The goal of the research to be performed at the new laboratory is to accelerate the creation of source material, cultivars and hybrids by combining traditional breeding methods and cell technologies. The objects of the study include cultivated forms and wild relatives of cabbage Brassica oleracea L., turnip Brassica rapa L., lettuce Lactuca L., tomato Lycopersicon Mill and vegetable sweet corn Zea mays var. saccharata Sturt. In the present review, we consider the main results of breeding cabbage, tomato, and lettuce which have been obtained through applying cell technologies. Despite the progress obtained, there are still several problems in this area. The lack of standardized, efficient and reproducible protocols for in vitro methods often hinders their practical use. The tasks facing the laboratory in creating the initial breeding material and new cultivars and hybrids with the use of both conventional methods and cell technologies are relevant and correspond to the world level.

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Section: Introductionunclassified

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Possibilities of biotechnological methods in breeding of vegetable crops at the VIR Laboratory of Breeding and Cell Technologies

Курина

Artemyeva

2023

Biotehnologiâ i selekciâ rastenij

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“…In vitro culture of microspores is a multi-stage complex technology, and a huge number of factors influence its efficiency [6,8]. One of the most important factors is the stage of microspore development.…”

Section: Introductionmentioning

confidence: 99%

“…One of the most important factors is the stage of microspore development. Numerous studies have shown that microspores at the late unicellular vacuolized stage and pollen at the early bicellular stage (for convenience, we will refer to both these stages as microspores) can change their developmental pathway from gametophytic to sporophytic [8]. Since only these microspore stages are capable of embryogenesis, microspores at other developmental stages die during cultivation and have a toxic effect on the cell culture [9].…”

Section: Introductionmentioning

confidence: 99%

Effect of the Method of Microspore Isolation on the Efficiency of Isolated Microspore Culture In Vitro for Brassicaceae Family

Kozar

Домблидес

2022

Horticulturae

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Isolated microspore culture in vitro (IMC) is an advanced technique for producing doubled haploids. We developed a modified microspore isolation method for the Brassicaceae family, which exceeds the results obtained by a standard microspore isolation method. We found that the new method allows an increase in the percentage of microspores at the embryogenic stage of development in the culture. In the spring rapeseed ‘Ratnik’ culture the percentage of microspores increases from 66.7% to 73%, and in the European radish ‘RBK’ from 34% to 61.9%. Moreover, the new method of microspore isolation made it possible to expand the range of linear bud sizes (from 3.5–4.0 to 3.0–4.5 mm for spring rapeseed ‘Ratnik’) suitable for IMC technology. In addition, the new method of microspore isolation reduced the debris in the preparation of spring rapeseed ‘Ratnik’ and European radish ‘RBK’ by 2.4 and 15 times, respectively. The best results were shown on Sareptian mustard No. 72, where the yield of embryoids increased by 7.5 times. Remarkably, the new method of microspore isolation allowed us to obtain the first embryoids of red cabbage No. 428, whereas no embryoids were obtained using the standard method of microspore isolation. In summary, the new method of microspore isolation allows an increase in the efficiency of IMC technology for Brassicaceae family crops.

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Isolated microspore culture for embryoid production in Artemisia annua L.

Purnamaningsih,

Dewi,

Sukmadjaja

et al. 2024

Plant Cell Tiss Organ Cult

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The haploidy technique is a useful tool to produce pure, fully homozygous lines in a short time. Artemisia annua (L) is a medicinal plant that produces artemisinin widely known as an anti-malarial drug. Due to its very small ower (< 3.0 mm), this study aimed to conduct microscopic observations to determine the type of owers and their corresponding microspore development stages suitable for microspore embryogenesis and to obtain embryoids from isolated microspore cultures. Media to induce embryoid production were based on NLN medium with 13% or 17% sucrose and the addition of plant growth regulators as follows: (1) a combination of NAA and BA, designated as MCA media; and (2) a combination of 2,4-D and Kinetin, designated as MCAD media. The result indicated that due to their proportion of uninucleate and binucleate microspore stages, owers at about to bloom (AB) and early bloom (EB) stages contained su cient microspore stages of late uninucleate to early binucleate microspores suitable to induce embryogenesis. The production of embryoid is faster in MCA13 and MCA17 than those of MCAD13 and MCAD17. MCA13 and MCAD13 gave rise to abundant callus-like structures than those of MCA17 and MCAD17. It is indicated that the addition of 2.4-D in MCAD media inhibited the growth of the embryoid, due to the formation of a white-gluey structure covered and mixed with the embryoid. Key messageThis is the rst report of successful induction of embryoids in Artemisia annua from isolated microspore culture, which is closely related to ower type and stage of microspore development.

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Influencing factors and physiochemical changes of embryogenesis through in vitro isolated microspore culture in Brassica species

Cited by 7 publications

References 240 publications

Possibilities of biotechnological methods in breeding of vegetable crops at the VIR Laboratory of Breeding and Cell Technologies

Possibilities of biotechnological methods in breeding of vegetable crops at the VIR Laboratory of Breeding and Cell Technologies

Effect of the Method of Microspore Isolation on the Efficiency of Isolated Microspore Culture In Vitro for Brassicaceae Family

Isolated microspore culture for embryoid production in Artemisia annua L.

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