Influences of Xeno-Free Media on Mesenchymal Stem Cell Expansion for Clinical Application

Bui, Hue Thi Hong; Nguyen, Liem Thanh; Than, Uyen Thi Trang

doi:10.1007/s13770-020-00306-z

Cited by 35 publications

(30 citation statements)

References 50 publications

(148 reference statements)

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“…However, the CD 90- and 105-positive cells were less than 80% and did not meet the IFATS criteria. This might indicate a heterogeneous population and/or the immune-stimulated status of the cells [ 62 ]. Interestingly, the percentage of CD 105 in the XSF group was only 2.94 ± 1.29%, which was significantly less than that in the FBS group.…”

Section: Discussionmentioning

confidence: 99%

A Three-Dimensional Printed Polycaprolactone–Biphasic-Calcium-Phosphate Scaffold Combined with Adipose-Derived Stem Cells Cultured in Xenogeneic Serum-Free Media for the Treatment of Bone Defects

Supphaprasitt

Charoenmuang

Thuaksuban

et al. 2022

JFB

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The efficacy of a three-dimensional printed polycaprolactone–biphasic-calcium-phosphate scaffold (PCL–BCP TDP scaffold) seeded with adipose-derived stem cells (ADSCs), which were cultured in xenogeneic serum-free media (XSFM) to enhance bone formation, was assessed in vitro and in animal models. The ADSCs were isolated from the buccal fat tissue of six patients using enzymatic digestion and the plastic adherence method. The proliferation and osteogenic differentiation of the cells cultured in XSFM when seeded on the scaffolds were assessed and compared with those of cells cultured in a medium containing fetal bovine serum (FBS). The cell–scaffold constructs were cultured in XSFM and were implanted into calvarial defects in thirty-six Wistar rats to assess new bone regeneration. The proliferation and osteogenic differentiation of the cells in the XSFM medium were notably better than that of the cells in the FBS medium. However, the efficacy of the constructs in enhancing new bone formation in the calvarial defects of rats was not statistically different to that achieved using the scaffolds alone. In conclusion, the PCL–BCP TDP scaffolds were biocompatible and suitable for use as an osteoconductive framework. The XSFM medium could support the proliferation and differentiation of ADSCs in vitro. However, the cell–scaffold constructs had no benefit in the enhancement of new bone formation in animal models.

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Section: Discussionmentioning

confidence: 99%

A Three-Dimensional Printed Polycaprolactone–Biphasic-Calcium-Phosphate Scaffold Combined with Adipose-Derived Stem Cells Cultured in Xenogeneic Serum-Free Media for the Treatment of Bone Defects

Supphaprasitt

Charoenmuang

Thuaksuban

et al. 2022

JFB

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“…Furthermore, ADSC have been successfully cultured with allogenic platelet lysate generated by freeze-thawing of human platelet concentrates ( 76 – 79 ). However, as the use of hPL has also economic and regulatory concerns, future efforts are directed towards developing standardized GMP-grade formulation with recombinant bioactive molecules to “compensate” for the reduction or lack of serum ( 76 , 77 ).…”

Section: Isolation and Expansion Of Mscs Derived From Different Sourcesmentioning

confidence: 99%

Optimization of Mesenchymal Stromal Cell (MSC) Manufacturing Processes for a Better Therapeutic Outcome

et al. 2022

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MSCs products as well as their derived extracellular vesicles, are currently being explored as advanced biologics in cell-based therapies with high expectations for their clinical use in the next few years. In recent years, various strategies designed for improving the therapeutic potential of mesenchymal stromal cells (MSCs), including pre-conditioning for enhanced cytokine production, improved cell homing and strengthening of immunomodulatory properties, have been developed but the manufacture and handling of these cells for their use as advanced therapy medicinal products (ATMPs) remains insufficiently studied, and available data are mainly related to non-industrial processes. In the present article, we will review this topic, analyzing current information on the specific regulations, the selection of living donors as well as MSCs from different sources (bone marrow, adipose tissue, umbilical cord, etc.), in-process quality controls for ensuring cell efficiency and safety during all stages of the manual and automatic (bioreactors) manufacturing process, including cryopreservation, the use of cell banks, handling medicines, transport systems of ATMPs, among other related aspects, according to European and US legislation. Our aim is to provide a guide for a better, homogeneous manufacturing of therapeutic cellular products with special reference to MSCs.

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“…Despite the success obtained in experimental studies that used alternatives to animal serum in MSC cultures, further studies are needed for MSC therapeutic applications. Regardless of the present xenon-free media consisting of defined components, there are limitations for their use in MSC cultivation [ 114 , 115 ].…”

Section: Future Possible Solutionsmentioning

confidence: 99%

Serum-Free Cultures: Could They Be a Future Direction to Improve Neuronal Differentiation of Mesenchymal Stromal Cells?

Schepici

Mazzon

2022

IJMS

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Mesenchymal stem/stromal cells (MSCs) are undifferentiated cells with multilinear potential, known for their immunomodulatory and regenerative properties. Although the scientific community is working to improve their application, concerns limit their use to repair tissues following neurological damage. One of these obstacles is represented by the use of culture media supplemented with fetal bovine serum (FBS), which, due to its xenogenic nature and the risk of contamination, has increased scientific, ethical and safety problems. Therefore, the use of serum-free media could improve MSC culture methods, avoiding infectious and immunogenic transmission problems as well as MSC bioprocesses, without the use of animal components. The purpose of our review is to provide an overview of experimental studies that demonstrate that serum-free cultures, along with the supplementation of growth factors or chemicals, can lead to a more defined and controlled environment, enhancing the proliferation and neuronal differentiation of MSCs.

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Influences of Xeno-Free Media on Mesenchymal Stem Cell Expansion for Clinical Application

Cited by 35 publications

References 50 publications

A Three-Dimensional Printed Polycaprolactone–Biphasic-Calcium-Phosphate Scaffold Combined with Adipose-Derived Stem Cells Cultured in Xenogeneic Serum-Free Media for the Treatment of Bone Defects

A Three-Dimensional Printed Polycaprolactone–Biphasic-Calcium-Phosphate Scaffold Combined with Adipose-Derived Stem Cells Cultured in Xenogeneic Serum-Free Media for the Treatment of Bone Defects

Optimization of Mesenchymal Stromal Cell (MSC) Manufacturing Processes for a Better Therapeutic Outcome

Serum-Free Cultures: Could They Be a Future Direction to Improve Neuronal Differentiation of Mesenchymal Stromal Cells?

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