Influences of two antibiotic contaminants on the production, release and toxicity of microcystins

Liu, Ying; Gao, Baoyu; Yue, Qinyan; Guan, Yuntao; Wang, Yan; Huang, Lihui

doi:10.1016/j.ecoenv.2011.10.027

Cited by 74 publications

(50 citation statements)

References 74 publications

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“…One concern in using algicides to treat CyanoHABs is that they may increase the production and release of cyanotoxins (7,33,63), which may increase toxin levels and aggravate rather than solve water quality problems (63,64). As has been observed, CuSO 4 , tryptamine, and tryptoline caused similar increases in the intracellular, extracellular, and total MC-LR contents in Microcystis sp.…”

Section: Discussionsupporting

confidence: 58%

“…Over 90 variants of MCs have been identified so far (3,5), among which microcystin-LR (MC-LR) is the most common and toxic. MC-LR accounts for 45.5 to 99.8% of the MCs in bloom-impacted natural waters (6) and 57% of MCs produced by Microcystis aeruginosa cultures (7). Therefore, MC-LR often serves as the model for studies related to microcystin production, degradation, and toxicity (2,(4)(5)(6)(7)(8).…”

mentioning

confidence: 99%

“…For example, lysine can cause severe damage to the cell wall of M. aeruginosa (28); tryptamine and gramine can reduce chlorophyll a (Chl-a) content, degrade superoxide dismutase (SOD), and induce the production of reactive oxygen species (ROS) and malondialdehyde (MDA) in cyanobacterial cells (14,27). Some algicidal compounds, such as amoxicillin and spiramycin, can also affect intracellular cyanotoxin synthesis (7).…”

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confidence: 99%

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Algicidal Activity of Streptomyces eurocidicus JXJ-0089 Metabolites and Their Effects on Microcystis Physiology

Zhang

Ding

et al. 2016

Appl Environ Microbiol

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Copper sulfate (CuSO 4 ) has been widely used as an algicide to control harmful cyanobacterial blooms (CyanoHABs) in freshwater lakes. However, there are increasing concerns about this application, due mainly to the general toxicity of CuSO 4 to other aquatic species and its long-term persistence in the environment. This study reported the isolation and characterization of two natural algicidal compounds, i.e., tryptamine and tryptoline, from Streptomyces eurocidicus JXJ-0089. At a concentration of 5 g/ml, both compounds showed higher algicidal efficiencies than CuSO 4 on Microcystis sp. FACHB-905 and some other harmful cyanobacterial strains. Tryptamine and tryptoline treatments induced a degradation of chlorophyll and cell walls of cyanobacteria. These two compounds also significantly increased the intracellular oxidant content, i.e., superoxide anion radical (O 2 ؊ ) and malondialdehyde (MDA), but reduced the activity of intracellular reductants, i.e., superoxide dismutase (SOD), of cyanobacteria. Moreover, tryptamine and tryptoline treatments significantly altered the internal and external contents of microcystin-LR (MC-LR), a common cyanotoxin. Like CuSO 4 , tryptamine and tryptoline led to releases of intracellular MC-LR from Microcystis, but with lower rates than CuSO 4 . Tryptamine and tryptoline (5 g/ml) in cyanobacterial cultures were completely degraded within 8 days, while CuSO 4 persisted for months. Overall, our results suggest that tryptamine and tryptoline could potentially serve as more efficient and environmentally friendly alternative algicides than CuSO 4 in controlling harmful cyanobacterial blooms. IMPORTANCECyanobacterial harmful algal blooms (CyanoHABs) in aquatic environments have become a worldwide problem. Numerous efforts have been made to seek means to prevent, control, and mitigate CyanoHABs. Copper sulfate (CuSO 4 ), was once a common algicide to treat and control CyanoHABs. However, its application has become limited due to concerns about its general toxicity to other aquatic species and its long-term persistence in the environment. There is a great need for algicides with higher specificity and low environmental impacts. This study reports the isolation and characterization of two natural algicidal compounds from a streptomycete strain, Streptomyces eurocidicus JXJ-0089. Our results suggest that the identified algicides could potentially serve as more efficient and environmentally friendly alternative algicides than CuSO 4 in controlling harmful cyanobacterial blooms. Harmful cyanobacterial blooms (CyanoHABs) have become a global phenomenon and are occurring with increasing intensity, area of infection, and frequency (1). One danger that CyanoHABs have posed to the environment and human health is the production and release of cyanotoxins. The most frequently detected cyanotoxins in freshwater systems are microcystins (MCs), a group of potent liver toxins (2, 3). An MC contamination incident in 1996 caused the death of 53 patients in Caruaru, Brazil (4). A more recent MC c...

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Section: Discussionsupporting

confidence: 58%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Algicidal Activity of Streptomyces eurocidicus JXJ-0089 Metabolites and Their Effects on Microcystis Physiology

Zhang

Ding

et al. 2016

Appl Environ Microbiol

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“…Acute toxicity (LC50) of furazolidone was found at 40 mg/kg for the mosquito larvae Culex pipiens [27]. Amoxicillin exerts different toxicities on different species of microalgae and aquatic plants [21].…”

Section: Toxicity Of the Antibiotics To M Aeruginosamentioning

confidence: 99%

“…Previously, we demonstrated that the herbicide diclofop acid can affect the production and release of MC-LR in M. aeruginosa [35]. Liu et al [21] reported that spiramycin and amoxicillin can affect the production and release of MCs in M. aeruginosa. The present study found that CTC, OTC and TC interfered the production of MC-LR to different degrees, with different manners, and most MC-LR equivalents mainly in the intracellular fraction at 48 h of exposure.…”

Section: Influence Of Antibiotics On Mc-lr Production and Release In mentioning

confidence: 99%

Toxin Release of Cyanobacterium <em>Microsystis aeruginosa</em> after Exposure to Typical Tetracycline Antibiotic Contaminants

Wang

et al. 2017

Preprint

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The global usage of veterinary antibiotics is significant. Antibiotics can be released into aquatic environments and elicit toxic effects on non-target organisms. In this study, the growth characteristics and toxin release of the cyanobacterium Microcystis aeruginosa (M. aeruginosa) were examined to investigate the physiological effects of tetracycline antibiotics on aquatic life. Results showed that the degree of toxicities of the following target antibiotics was TC (tetracycline hydrochloride) > CTC (chlortetracycline hydrochloride) > OTC (oxytetracycline hydrochloride) in terms of growth parameters, EC10 (0.63, 1.86, and 3.02 mg/L, respectively), and EC20 (1.58, 4.09, and 4.86 mg/L, respectively) values. These antibiotics inhibited the production of microcystin-LR (MC-LR) to varying degrees. CTC interfered M. aeruginosa cells and decreased their ability to release MC-LR, but this antibiotic stimulated the ability of these cells to synthesize MC-LR at 2 and 5 mg/L. OTC elicited a relatively weaker toxicity than CTC did and reduced MC-LR release. TC was the most toxic among the three antibiotics, and this antibiotic simultaneously reduced intracellular and extracellular MC-LR equivalents. Our results helped elucidate the effects of tetracycline antibiotics on M. aeruginosa, which is essential for environmental evaluation and protection. Our results are also helpful for guiding the application of veterinary antibiotics in agricultural settings.

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Toxicity evaluation of β‐diketone antibiotics on the development of embryo‐larval zebrafish (Danio rerio)

Wang

Che

Duan

et al. 2013

Environmental Toxicology

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This study evaluated the effects of β-diketone antibiotics (DKAs) on the development of embryo-larval zebrafish (Danio rerio). When exposure to DKAs, developmental malformations, such as hatching delay, curved body axis, pericardial edema, uninflated swim bladder and yolk sac edema, were observed at 120 h postfertilization (hpf). The estimated 120 hpf nominal concentrations of no observed effect concentration and lowest observed effect concentration for DKAs were 18.75 and 37.50 mg/L, respectively, suggesting that DKAs have much lower toxicity than other persistent pollutants. Following DKA exposure, embryonic heart rates were significantly reduced as compared to the controls at 48 and 60 hpf. The peak bending motion frequency appeared 1 h earlier than in control embryos. The 2.34 and 9.38-mg/L treatment groups had a higher basal swim rate than control groups at 120 hpf in both light and light-to-dark photoperiod experiments. The occurrence of high speed swim rates was enhanced approximately threefold to sevenfold in the 2.34 and 9.38 mg/L treatments compared to the control. Glutathione (GSH) concentrations in the 2.34 and 9.38-mg/L treatments were significantly higher than the control at 72 hpf, suggesting that GSH production was induced at the end of the hatching period. When exposed to DKAs, zebrafish superoxide dismutase enzyme (SOD) activities were significantly inhibited in the early embryonic period, demonstrating that the clearing ability in zebrafish was lower than the generation rate of free radicals. In summary, the combined DKAs were developmentally toxic to zebrafish in their early life stages and had the ability to impair individual behaviors that are of great importance in the assessment of their ecological fitness.

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Influences of two antibiotic contaminants on the production, release and toxicity of microcystins

Cited by 74 publications

References 74 publications

Algicidal Activity of Streptomyces eurocidicus JXJ-0089 Metabolites and Their Effects on Microcystis Physiology

Algicidal Activity of Streptomyces eurocidicus JXJ-0089 Metabolites and Their Effects on Microcystis Physiology

Toxin Release of Cyanobacterium <em>Microsystis aeruginosa</em> after Exposure to Typical Tetracycline Antibiotic Contaminants

Toxicity evaluation of β‐diketone antibiotics on the development of embryo‐larval zebrafish (Danio rerio)

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