Influences of the alcoholic extract of Artemisia annua on gastrointestinal microbiota and performance of Nile tilapia

Soares, Michelly Pereira; Cardoso, Israel Luz; Araújo, Fabrício Eugênio; Angelis, Carolina Fernandes De; Mendes, Rodrigo; Mendes, Lucas William; Fernandes, Marisa Narciso; Jonsson, Cláudio Martín; Queiroz, S. C. do N. de; Duarte, Marta Cristina Teixeira; Rantin, Francisco Tadeu; Sampaio, Fernanda Garcia

doi:10.1016/j.aquaculture.2022.738521

Cited by 9 publications

(3 citation statements)

References 85 publications

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“…Intestinal villi play an important role in the digestion and absorption of nutrients, and the longer the villi are, the larger the area of the absorption site and the stronger the digestion capacity [ 34 ]. Crypt depth reflects the ability of intestinal cells to differentiate into villi [ 35 , 36 ]. In this study, the villus height and crypt depth in the experimental group showed an upward trend compared with those in the control group, indicating that C. butyricum can improve the E. sinensis intestinal tissue and improve the digestion and absorption abilities of the body.…”

Section: Discussionmentioning

confidence: 99%

Effects of Clostridium butyricum on Intestinal Microflora and Metabolism of Eriocheir sinensis

Gao,

Liu,

Wang

et al. 2023

IJMS

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Clostridium butyricum, a new probiotic in recent years, can produce butyric acid and short-chain fatty acids. It has the characteristics of strong acid and alkali resistance, high temperature resistance, and strong resistance to most antibiotics, and has more advantages than other probiotics. However, the action mechanism of C. butyricum on Eriocheir sinensis is still unclear and needs further study. In this study, when C. butyricum was added to the basic diet, the number of living bacteria was 0, 1 × 106 and 1 × 108 CFU/g, respectively. The E. sinensis were randomly divided into three groups: (blank control group, experimental group 1 (1 × 106 CFU/g) and experimental group 2 (1 × 108 CFU/g)). They were fed an experimental diet for 28 days. The effects of C. butyricum on E. sinensis were studied by detecting the differences in non-specific immune indexes, intestinal microflora, and metabolites between serum and hepatopancreas. The results showed that C. butyricum could improve the antioxidant ability of E. sinensis serum and hepatopancreas, protect intestinal tissues, and promote the absorption of nutrients. At the same time, it can enhance the microbial diversity and richness of the E. sinensis gut flora. LC-MS metabolomics was used to detect the metabolism of intestinal flora. It was found that C. butyricum could up-regulate lysophosphatidylcholine in the intestine. Through the KEGG enrichment pathway, it was found that significantly different metabolites were mainly concentrated in six metabolic pathways. The purine metabolism and alanine, aspartate, and glutamate metabolism pathways showed a downward trend, indicating that the addition of C. butyricum to feed could reduce purine metabolism, promote the water-salt balance of the organism’s cells, and reduce inflammation. In this study, it was found that the addition of certain concentrations of C. butyricum to feed could improve the antioxidant ability of E. sinensis, improve the intestinal flora environment, and promote the growth of beneficial bacteria in the gut. This can promote the body’s metabolism, which is more conducive to its growth.

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Section: Discussionmentioning

confidence: 99%

Effects of Clostridium butyricum on Intestinal Microflora and Metabolism of Eriocheir sinensis

Gao,

Liu,

Wang

et al. 2023

IJMS

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“…In addition, adding A. annua and its extracts into diets can effectively relieve heat stress, immune stress and oxidative stress in broilers ( Song et al, 2017 , 2018 ; Wan et al, 2017 , 2018 ; Choi et al, 2020 ). Addition of A. annua extract to Nile tilapia diets modulated the intestinal microbiota and altered the abundance of specific bacteria ( Fusobacteriaceae , Stenotrophomonas , and Clostridium ), thereby improving growth performance ( Soares et al, 2022 ). Notably, the polysaccharide, being a bioactive substance in A. annua , has been found to possess antitumor activity ( Yan et al, 2019 ), along with antioxidant and immunomodulatory properties, as confirmed by in vitro experiments ( Zhang et al, 2022a ).…”

Section: Introductionmentioning

confidence: 99%

Artemisia annua L. polysaccharide improves the growth performance and intestinal barrier function of broilers challenged with Escherichia coli

Guo,

Shi,

Xing

et al. 2024

Front. Microbiol.

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With the high intensification of poultry breeding, a series of diseases caused by pathogenic bacteria threaten the health of poultry and human. Among them, poultry diseases induced by Escherichia coli cause significant economic loss every year. The aim of this study was to investigate the effects of dietary supplementation with Artemisia annua L. polysaccharide (AAP) on the growth performance and intestinal barrier function of broilers with Escherichia coli (E. coli) challenge. A total of 256 one-day-old chicks were randomly assigned to four treatment groups: control group (fed basal diet), AAP group (fed basal diet supplemented with AAP), E. coli group (fed basal diet and orally administered E. coli), AAP + E. coli group (fed basal diet supplemented with AAP and orally administered E. coli). Dietary AAP supplementation elevated the BW, ADG and ADFI in non-challenged broilers. AAP also increased the apparent metabolic rate of EE and Ca in E. coli-challenged broilers. Moreover, AAP not only enhanced the serum IgA content but also decreased the serum and jejunum content of IL-6, as well as the jejunum level of IL-1β in non-challenged broilers. AAP also down-regulates the mRNA level of inflammatory factors (IL-1β, IL-6, and TNF-α) by inhibiting the mRNA expression of TLR4 and MyD88 in intestinal NF-κB signaling pathway of E. coli-challenged broilers. Meanwhile, AAP up-regulates the activity and mRNA level CAT by down-regulating the mRNA level of Keap1 in intestinal Nrf2 signaling pathway of E. coli-challenged broilers, and decreased serum MDA concentration. AAP significantly elevated the mRNA level of CAT, SOD and Nrf2 in jejunal of non-challenged broilers. Interestingly, AAP can improve intestinal physical barrier by down-regulating serum ET content, increasing the jejunal villus height/crypt depth (VH/CD) and ZO-1 mRNA level in broilers challenged by E. coli. AAP also elevated the VH/CD and the mRNA level of Occludin, ZO-1, Mucin-2 in non-challenged broilers. Importantly, AAP reshaped the balance of jejunum microbiota in E. coli-challenged broilers by altering α diversity and community composition. In summary, AAP ameliorated the loss of growth performance in broilers challenged with E. coli, probably by regulating the intestinal permeability and mucosa morphology, immune function, antioxidant ability, and microbiota.

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“…In addition, the supplementation of 2 mg/kg of ART could reduce intestinal inflammation in weaned piglets and enhance intestinal immunity and digestive capacity ( 17 ). There has been increasing interest in recent years in improving animal health through the use of ART additives, which have positive effects on various fish species, including common carp ( Cyprinus carpio ) ( 18 ), rainbow trout ( Oncorhynchus mykiss ) ( 19 ), Nile tilapia ( Oreochromis niloticus ) ( 20 ), Mozambique tilapia ( Oreochromis mossambicus ) (Mbokane and Moyo, 2018), African catfish ( Clarias gariepinus ) ( 21 ), and largemouth bass ( Micropterus salmoides ) ( 22 ). For instance, the supplementation of ART to the diet of Litopenaeus vannamei significantly ameliorated Vibrio parahaemolyticus- induced intestinal inflammation and poor histomorphology ( 23 ).…”

Section: Introductionmentioning

confidence: 99%

Dietary artemisinin boosts intestinal immunity and healthy in fat greenling (Hexagrammos otakii)

Wang

Zhan

et al. 2023

Front. Immunol.

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IntroductionArtemisinin (ART) is very common as a diet additive due to its immunoregulatory activities. Nonetheless, the immunoregulatory mechanism of ART in marine fish remains unknown. This study comprehensively examined the effects and explored the potential mechanism of ART ameliorating intestinal immune disease (IID) in fat greenlings (Hexagrammos otakii).Methods and resultsThe targets of ART were screened using the Traditional Chinese Medicine Systems Pharmacology (TCMSP) database. Here, eight putative targets of ART were collected and identified with the Uniprot database, and 1419 IID-associated target proteins were filtered through the Drugbank, Genecards, OMIM, and PHARMGKB Databases. The results of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways point out that ART may have immunoprotective effects by regulating cellular responses to stress, hypoxia, inflammation, and vascular endothelial growth factor stimulus through the hypoxia-inducible factor 1 (HIF-1) signaling pathway. The findings of molecular docking indicated that ART contains one active ingredient and three cross-targets, which showed a kind combination with hypoxia-inducible factor 1-alpha (HIF1-a), transcription factor p65 (RELA), and vascular endothelial growth factor A (VEGF-A), respectively. Furthermore, an ART feeding model was established to assess the ART’s immunoprotect effect on the intestine of H.otakii in vivo. The D48 group showed smaller intestinal structural changes after being challenged by Edwardsiella tarda. The supplementation of ART to the diet improved total superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) and reduced the malondialdehyde (MDA) in intestine of H. otakii. The expression of transcription factor p65, HIF1-α, VEGF-A, cyclin D1, matrix metalloprotease 9 (MMP9), monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6) was decreased after dietary ART in the intestinal of H. otakii.DiscussionThe present results demonstrated that dietary ART improved antioxidants and immunity, optimized the intestinal structure, and increased resistance to E. tarda through the SOD2/nuclear-factor-kappa- B (NFkB)/HIF1-a/VEGF-A pathway in the intestinal tract of H.otakii. This study integrated pharmacological analysis and experimental validation and revealed the mechanism of ART on IID, which provides insight into the improvement of IID in H. otakii.

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Influences of the alcoholic extract of Artemisia annua on gastrointestinal microbiota and performance of Nile tilapia

Cited by 9 publications

References 85 publications

Effects of Clostridium butyricum on Intestinal Microflora and Metabolism of Eriocheir sinensis

Effects of Clostridium butyricum on Intestinal Microflora and Metabolism of Eriocheir sinensis

Artemisia annua L. polysaccharide improves the growth performance and intestinal barrier function of broilers challenged with Escherichia coli

Dietary artemisinin boosts intestinal immunity and healthy in fat greenling (Hexagrammos otakii)

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