Influences of some biotic and abiotic factors on protein production and as inducers of Fusarium wilt disease resistance in lupine (Lupinus albus L.)

Abstract

Two bacterial species; Pseudomonas fluorescens and Pseudomonas putida and three different chemical compounds; copper sulphate (CuSO 4), Indole butyric acid (IBA) and potassium chloride (KCl) were tested for their ability to induce resistance in lupine plants against wilt disease caused by Fusarium oxysporum f. sp. lupini. Treatment of seeds with the selected bacterial species and chemical compounds significantly reduced wilt disease incidence of Fusarium oxysporum f. sp. in Lupine under greenhouse conditions. Potassium chloride and Pseudomonas fluorescens were most effective. Sodium dodecyl sulphate-polyacrylamide gel electrophresis (SDS-PAGE) analysis of lupine seedlings revealed that seeds treated with biotic and abiotic inducers resulted in a rapid induction of different novel Pathogenesis-related protein (PR) in shoot and root of lupine seedlings upon infection with the pathogen. These new proteins were not detected in untreated healthy or infected controls. This study aimed to use bacterial species and chemical compounds to decrease Fusarium wilt disease.

Effectiveness of Bio-agent Formulations on Suppression of Lupine wilt Caused by Fusarium oxysporum f. sp. lupini

“…to pigeon pea reduces the wilting index. Furthermore, the current results are consistent with those of Mohamed et al (2013), who demonstrated that soaking lupine seeds in P. fluorescens suspension effectively induced a suppressive response against F. oxysporum f. sp. lupini.…”