Influences of pH and Iron Concentration on the Salivary Microbiome in Individual Humans with and without Caries

Zhou, Jianye; Jiang, Nan; Wang, Zhengzheng; Li, Longqing; Zhang, Jumei; Ma, Rui; Nie, Huan; Li, Zhiqiang

doi:10.1128/aem.02412-16

Cited by 41 publications

(39 citation statements)

References 53 publications

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“…On the other hand, a recent study also showed that systemic diseases have significant influence on the oral microbiota [22]. Salivary urate is reported to be significantly increased in gout and higher concentrations may change the oral microenvironment, such as the pH of saliva [13], which has been demonstrated to be an important factor that affects the balance of oral microbial communities [23,24]. In addition to uric acid, there might be many other metabolites that are altered in the saliva of gout/HUA patients that contribute to the modification of the normal oral microbiota.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Oral Microbiota Revealed High Abundance of Prevotella Intermedia in Gout Patients

Liu

Cui

Yan

et al. 2018

Cell Physiol Biochem

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Background/Aims: Microbes reside in a number of body sites, including the oral cavity, and are associated with the progression of many systemic diseases. In this study, we aimed to investigate the effects of gout and hyperuricemia (HUA) on the composition of oral microbiomes. Methods: Analysis of the oral microbiota from 12 gout patients, 11 HUA patients, and 19 healthy control subjects was performed using a deep sequencing approach, and validation of significant changes in Prevotella intermedia and Serratia marcescens in new patient cohorts was performed using quantitative PCR (qPCR). Results: Our analysis indicated that both gout and HUA significantly altered the composition of the oral microbiome in patients. Patients with gout or HUA had significantly greater levels of salivary Prevotella intermedia but significantly lower levels of Serratia marcescens than healthy control subjects. Conclusion: We demonstrated the association between the oral microbiome and gout and HUA for the first time. In particular, 16S sequencing and qPCR analysis revealed significantly higher levels of oral Prevotella intermedia in gout/HUA patients, which suggests that these patients might be at risk for the development of periodontitis.

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Section: Discussionmentioning

confidence: 99%

Analysis of Oral Microbiota Revealed High Abundance of Prevotella Intermedia in Gout Patients

Liu

Cui

Yan

et al. 2018

Cell Physiol Biochem

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“…The oral cavity is one of the most complex and largest microbial habitats that harbors hundreds of different bacteria which play important roles in maintaining oral homeostasis and influencing the development of both oral and systematic diseases [4]. Many factors in the oral environment including intraoral pH and salivary iron concentration as well as expression of serum malondialdehyde and lipid profile have been reported to have significant relationships with oral microbial communities [5,6]. Several recent studies detected the efficacy of antibiotics or drug composed of herbal on oral microbiol communities [7,8].…”

Section: Introductionmentioning

confidence: 99%

Analysis of by high-throughput sequencing: Helicobacter pylori infection and salivary microbiome

Liang

Lü

2020

BMC Oral Health

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Background: There have been reports of Helicobacter pylori (H. pylori) in the oral cavity and it has been suggested that the oral cavity may be a reservoir for H. pylori reflux from the stomach. High-throughput sequencing was used to assess the structure and composition of oral microbiota communities in individuals with or without confirmed H. pylori infection. Methods: Saliva samples were obtained from 34 H. pylori infected and 24 H. pylori uninfected subjects. Bacterial genomic DNA was extracted and examined by sequencing by amplification of the 16S rDNA V3-V4 hypervariable regions followed by bioinformatics analysis. Saliva sampling was repeated from 22 of the 34 H. pylori infected subjects 2 months after H. pylori eradication. Results: High-quality sequences (2,812,659) clustered into 95,812 operational taxonomic units (OTUs; 97% identity). H. pylori was detected in the oral cavity in infected (12/34), uninfected (11/24) and eradicated (15/22) subjects by technique of high-throughput sequencing, occupying 0.0139% of the total sequences. Alpha diversity of H. pylori infected subjects was similar to that of uninfected subjects (

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“…The significant influences of the oral environment observed in this study increase the current understanding of the salivary microbiome in caries. These results will be useful for expanding research directions and for improving disease diagnosis, prognosis, and therapy [67].…”

Section: Role Of Diagnostic Propertiesmentioning

confidence: 92%

Functions of Saliva

Maddu¹

2019

Saliva and Salivary Diagnostics

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Saliva is produced and secreted by salivary glands. The basic secretary units of salivary glands are clusters of acini cells. It is fluid that contains water, electrolytes, mucus, and enzymes, all of which flow out of the acinus into collecting ducts, certainly one of the most important components and an integral component to oral health. The protective role and benefits including buffering, remineralization in the healthy oral mucosa, immune defense, digestion, lubrication, diagnostic purpose, and proteome analysis are fulfilled by saliva. It aids in maintaining mucosal integrity and indigestion through salivary enzymes. The functions of saliva in maintaining oral health and the main factors that cause alterations in salivary secretion and the importance of saliva in caries development and bacterial plaque formation are discussed, and also its role and functions and organic and inorganic constituents in saliva are discussed. This is of great importance in ruminants, which have non-secretory forestomachs. Diseases of the salivary glands and ducts are not uncommon in animals and man, and excessive salivation is a symptom of almost any lesions in the oral cavity.

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Influences of pH and Iron Concentration on the Salivary Microbiome in Individual Humans with and without Caries

Cited by 41 publications

References 53 publications

Analysis of Oral Microbiota Revealed High Abundance of Prevotella Intermedia in Gout Patients

Analysis of Oral Microbiota Revealed High Abundance of Prevotella Intermedia in Gout Patients

Analysis of by high-throughput sequencing: Helicobacter pylori infection and salivary microbiome

Functions of Saliva

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