Influences of different drying methods on the structural characteristics and multiple bioactivities of polysaccharides from okra (Abelmoschus esculentus)

Yuan, Qin; He, Yuan; Xiang, Pan-Yin; Huang, Yue-Jia; Cao, Zheng-Wen; Shen, Siwei; Zhao, Li; Zhang, Qing; Qin, Wen; Wu, Ding‐Tao

doi:10.1016/j.ijbiomac.2019.10.073

Cited by 67 publications

(38 citation statements)

References 37 publications

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“…We found that the molecular mass distribution was neither altered by spray drying nor by freeze drying, which is in line with a study on EPS from Lactobacillus helveticus where even higher inlet (160°C) and outlet (80°C) temperatures were applied during spray drying compared to our study (140°C and 58–62°C, respectively) [37]. For plant polysaccharides, however, a significant decrease of the molecular mass was observed already at lower temperatures by using spray drying with a rotary atomizer (inlet temperature 150°C and outlet temperature 78°C) or drying in an oven (12 h at 75°C) [46, 47]. For hot air drying performed at 50°C for 5 h, this was attributed to the rapid removal of bound water causing a breakdown of the polysaccharide structure [48].…”

Section: Resultssupporting

confidence: 91%

Pilot scale isolation of exopolysaccharides from Streptococcus thermophilus DGCC7710: Impact of methodical details on macromolecular properties and technofunctionality

Nachtigall

Surber

Bulla

et al. 2020

Engineering in Life Sciences

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Exopolysaccharides (EPS) from Streptococcus thermophilus provide similar technofunctionality such as water binding, viscosity enhancing and emulsifying effects as commercial thickeners at a significant lower concentration. Despite their high technofunctional potential, hetero polysaccharides from lactic acid bacteria are still not commercially used in unfermented foods, as the small amount of synthesised EPS calls for a high isolation effort. This study aims to analyse the macromolecular properties of EPS and cell containing isolates from S. thermophilus DGCC7710 obtained by different isolation protocols, and to link these data to the technofunctionality in model food systems. The EPS content of the isolates was affected by the microfiltration/ultrafiltration membranes used for cell removal/dialysis, respectively, and was 89% at maximum. There was no link between purity of the isolates, molecular mass (3 × 106 Da) and intrinsic viscosity (0.53 – 0.59 mL/mg) of the EPS. After adding EPS containing isolates to milk, gel stiffness after acidification increased by 25% at maximum, depending on the type and concentration of the specific isolate. Partly purified, cell containing isolates were effective at low absolute EPS concentration (approx. 0.1 g/kg) and therefore represent, together with their simple isolation protocol, an interesting approach to introduce microbial EPS into non‐fermented products.

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Section: Resultssupporting

confidence: 91%

Pilot scale isolation of exopolysaccharides from Streptococcus thermophilus DGCC7710: Impact of methodical details on macromolecular properties and technofunctionality

Nachtigall

Surber

Bulla

et al. 2020

Engineering in Life Sciences

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“…This phenomenon was possibly caused by the decrease of their molecular weights and changes of their monosaccharide compositions. The findings suggest that the apparent viscosities of DFP-H, DFP-M, DFP-U, and DFP-P are highly associated with their molecular weights, which is also reported by other studies [ 27 , 28 ].…”

Section: Resultssupporting

confidence: 86%

Physicochemical and Biological Properties of Polysaccharides from Dictyophora indusiata Prepared by Different Extraction Techniques

Zhao

Guo

et al. 2021

Polymers

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In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared. Results revealed that extraction yields of D. indusiata polysaccharides prepared by different extraction techniques ranged from 5.62% to 6.48%. D. indusiata polysaccharides prepared by different extraction techniques possessed similar chemical compositions and monosaccharide compositions, while exhibited different molecular weights (Mw), apparent viscosities, and molar ratios of constituent monosaccharides. In particularly, D. indusiata polysaccharides prepared by HWE (DFP-H) had the highest Mw and apparent viscosity among all DFPs, while D. indusiata polysaccharides extracted by UAE (DFP-U) possessed the lowest Mw and apparent viscosity. In addition, the in vitro antioxidant effects of D. indusiata polysaccharides prepared by PAE (DFP-P) and DFP-U were significantly higher than that of others. Indeed, both DFP-P and DFP-H exhibited much higher in vitro binding properties, including fat, cholesterol, and bile acid binding properties, and lipase inhibitory effects than that of D. indusiata polysaccharides prepared by MAE (DFP-M) and DFP-U. These findings suggest that the PAE technique has good potential for the preparation of D. indusiata polysaccharides with desirable bioactivities for the application in the functional food industry.

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“…Moreover, in comparison with the positive control, all PPPs exhibited strong α-glucosidase inhibitive effects and moderate α-amylase inhibitive effects. However, compared with polysaccharides obtained from okra [51], Momordica charantia [37], and green tea [52], both PPP-W and PPP-P exhibited higher α-amylase inhibition activities. Furthermore, the α-amylase and α-glucosidase inhibitory activities of PPPs were higher than those of polysaccharides obtained from Hovenia dulcis [10], but lower than those of flavonoids obtained from Hovenia dulcis [11], and the α-glucosidase and α-amylase inhibitive effects were also positively correlated with the contents of polysaccharides and flavonoids.…”

Section: In Vitro Inhibitory Activities On α-Amylase and α-Glucosidasementioning

confidence: 79%

Polyphenolic-Protein-Polysaccharide Complexes from Hovenia dulcis: Insights into Extraction Methods on Their Physicochemical Properties and In Vitro Bioactivities

Xian

et al. 2020

Foods

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Seven extraction methods, including hot water extraction (HWE), pressurized water extraction (PWE), ultrasound-assisted extraction, microwave-assisted extraction, ultrasound-assisted enzymatic extraction, high-speed shearing homogenization extraction, and ultrasound-microwave-assisted extraction, were utilized to extract polyphenolic-protein-polysaccharide complexes (PPPs) from Hovenia dulcis. Next, their physicochemical properties and in vitro antioxidant activities, antiglycation effects, and inhibition activities on α-glucosidase and α-amylase were studied and compared. The findings from this study indicate that various extraction processes exhibit notable influences on the physicochemical properties and in vitro bioactivities of PPPs. Extraction yields, contents of polyphenolics and flavonoids, apparent viscosities, molecular weights, molar ratios of monosaccharide compositions, and ratios of amino acid compositions in PPPs varied in different extraction methods. Furthermore, 13 phenolic compounds in PPPs, including rutin, myricitrin, myricetin, quercetin, kaempferol, protocatechuic acid, gallocatechin, p-hydroxybenzoic acid, ampelopsin, quercetin-7,4′-diglucoside, dihydroquercetin, 5-methylmyricetin, and naringenin, were identified. The relatively strong in vitro antioxidant activities, antiglycation effects, and inhibition activities on α-glucosidase and α-amylase were determined in both PPP-W and PPP-P obtained by HWE and PWE, respectively. The high content of total polyphenolics may be one of the main contributors to their in vitro bioactivities. The findings have shown that the PWE method can be an appropriate method to prepare PPPs with strong bioactivities for application in the functional food industry.

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Influences of different drying methods on the structural characteristics and multiple bioactivities of polysaccharides from okra (Abelmoschus esculentus)

Cited by 67 publications

References 37 publications

Pilot scale isolation of exopolysaccharides from Streptococcus thermophilus DGCC7710: Impact of methodical details on macromolecular properties and technofunctionality

Pilot scale isolation of exopolysaccharides from Streptococcus thermophilus DGCC7710: Impact of methodical details on macromolecular properties and technofunctionality

Physicochemical and Biological Properties of Polysaccharides from Dictyophora indusiata Prepared by Different Extraction Techniques

Polyphenolic-Protein-Polysaccharide Complexes from Hovenia dulcis: Insights into Extraction Methods on Their Physicochemical Properties and In Vitro Bioactivities

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