Influences of cultivar, cultivation year and fertilizer rate on amount of protein groups and amount and size distribution of mono- and polymeric proteins in wheat

Abstract

SU MMARYInfluences of cultivar and environment, i.e. cultivation year and fertilizer rate, on amount of protein groups and amount and size distribution of mono-and polymeric proteins, were investigated in four sets of wheat (Triticum aestivum L.). The cultivars were chosen in order to obtain a high range of variation in protein concentration and gluten strength. Environmental influences on protein concentration and gluten strength were investigated, as well as relations between variation in protein concentration and gluten strength and variation in protein groups and amount and size distribution of mono-and polymeric proteins.The results showed that cultivar and environmental influences giving rise to variation in protein concentration also gave rise to variation in most of the investigated protein components. Protein concentration was significantly positively correlated to the total amounts of glutenins and gliadins and amounts of most mono-and polymeric proteins. However, the correlation with the amount of gliadins and sodium dodecyl sulphate (SDS)-soluble mono-and polymeric proteins were often higher than the correlation to the glutenins and the SDS-insoluble mono-and polymeric proteins. Cultivar influences giving rise to variation in gluten strength were found to influence the relation between SDSsoluble and -insoluble polymeric proteins, leading to a significant positive correlation between the gluten strength and the percentage of total unextractable polymeric protein (TUPP) in the total polymeric protein and large unextractable polymeric protein (LUPP) in the total large polymeric protein. Environmental variation in gluten strength was found to be significantly positively correlated to SDS-insoluble proteins and negatively correlated to SDS-soluble proteins. This also led to a significant positive correlation with the percentage of LUPP and/or TUPP.

Effect of foliar application of Cu, Zn, and Mn on yield and quality indicators of winter wheat grain

“…Warechowska et al (2013) point out a mean Zeleny sedimentation index value of 31.4 cm 3 for popular wheat varieties cultivated in Europe. Johansson et al (2003) indicate that environmental conditions, including the years of the study, modify the amount and size distribution of mono-and polymeric proteins in the wheat grain. According to Konopka et al (2007b), the grain obtained under water deficit conditions contain less HMW and LMW glutenins, as well as albumins, globulins, and γ gliadins.…”

“…Many studies have shown a relationship between N fertilization and the deposition of storage proteins in the grain (Johansson et al, 2003;Labuschagne et al, 2006;Yue et al, 2007;Fuertes-Mendizábal et al, 2010;Chope et al, 2014); on the other hand, there is no information about the effects of extra micronutrient fertilization on fractional protein composition. Our study demonstrated that albumins and globulins (catalytic and structural proteins) accounted for 20.7% to 23.2% of total proteins.…”

“…Zecevic et al (2010), Campillo et al (2010), Piekarczyk et al (2011), and Mandic et al (2015) stated that increasing the N rate improves wheat grain quality, particularly gluten content and the sedimentation index. Johansson et al (2003) claim that both the varietal factor and mineral fertilizer rates have the greatest impact on protein groups (albumins, globulins, gliadins, high molecular weight [HMW], low molecular weight [LMW]) and glutenins. Labuschagne et al (2006) concluded that it is the N fertilizer rate and not the time of application that affects the fractional composition of proteins.…”

Zinc fertilization alters flour protein composition of winter wheat genotypes varying in gluten content

“…Flour quality is determined by both genetic and environmental factors (Johansson et al 2003). Many studies have reported that environmental conditions have a stronger effect on flour quality traits than genotype.…”

Adaptability and Genotype x Environment Interaction of Spring Wheat Cultivars in Chile using Regression Analysis, AMMI, and SRAG

“…These traits are defined through the combination of genetic and environmental factors such as soil characteristics, precipitation, fertilization, soil and air temperature, as well as the genotype × environment (G×E) interaction (Peterson et al, 1992;Johansson et al, 2003;Mellado, 2007). Some of the traits that define wheat grain quality are sedimentation and gluten content.…”