Influences of contamination of Aeromonas hydrophila, on quality, oxidative damage, and ultrastructure in cryopreserved sperm of the silver barb, Barbonymus gonionotus

Antioxidant Activity and Oxidative Stress-Oriented Apoptosis Pathway in Saccharides Supplemented Cryopreserved Sperm of Pacific Abalone, Haliotis discus hannai

“…•− production [28,33] or indirectly measuring lipid peroxidation (LPO) or malondialdehyde (MDA) as an oxidative stress marker [9,43]. Saccharides supplemented with P-CPA reduced O 2…”

“…Cryopreservation can lead to the generation of ROS such as superoxide anion (O 2 •− ), hydroperoxyl (HOO • ), and hydroxyl radicals ( • OH) responsible for sperm structure damage by reducing post-thaw motility, viability, and fertilizing ability [ 41 , 42 ]. The oxidative stress in post-thaw sperm can be evaluated by directly measuring O 2 • − production [ 28 , 33 ] or indirectly measuring lipid peroxidation (LPO) or malondialdehyde (MDA) as an oxidative stress marker [ 9 , 43 ]. Saccharides supplemented with P-CPA reduced O 2 •− and MDA levels in post-thaw sperm more than the control without supplementation of saccharides.…”

“…Despite its benefits, the freeze–thaw process of cryopreservation is known to generate excessive reactive oxygen species (ROS) [ 8 ]. Excessive accumulation of ROS leads to oxidative stress and reduce the motility, viability, and enzymatic activity of sperm [ 9 ]. Oxidative stress can also stimulate lipid peroxidation (LPO) and DNA fragmentation [ 10 ].…”

“…Sperm plasma membrane contains highly poly unsaturated fatty acids that are prone to oxidation by ROS or other free radicals. This oxidation can increase the level of membrane LPO that can be detected using malondialdehyde (MDA) indicator [ 9 ]. Antioxidant enzymes can counteract ROS production in cryopreservation [ 11 ].…”