Influences of biofluid sample collection and handling procedures on GC–MS based metabolomic studies

Current trends and challenges in sample preparation for global metabolomics using liquid chromatography–mass spectrometry

“…Heparin resulted in slightly more features (3,312 in positive ESI LC-MS) than EDTA (2,802) and sodium citrate (2,967), and method precision and number of features in negative mode ESI were very similar for all three anticoagulants. In contrast, the choice of anticoagulant used during blood collection was found to affect observed metabolic profiles in two other recent studies with reported differences in amino acids, lysophosphatidylcholine, and phosphatidylcholine [53,54]. In future studies, it would be interesting to study the differences in more detail and to determine whether the choice of anticoagulant affects the type of ions observed for a given metabolite; is there, for example, an increase in lithium adducts when using lithium heparin as anticoagulant?…”

Preanalytical Aspects and Sample Quality Assessment in Metabolomics Studies of Human Blood

“…One absolutely avoidable negative impact on sample quality is the choice of unsuitable blood collection tubes. Various, somewhat contradictory recommendations suggest that interference from the blood collection tubes may be manufacturer dependent (7,25,26 ). The exact tubes used for the study should therefore be tested beforehand, since chemical noise may greatly interfere with metabolic profiling.…”

Dissipation and residue determination of fluopyram and tebuconazole residues in watermelon and soil by GC-MS

“…Dong and J. Hu [19][20][21].Additionally, no information is available on simultaneous determination of fluopyram and tebuconazole in watermelon and soil by GC-MS. In this paper, a method based on Quick Easy Cheap Effective Rugged and Safe (QuEChERS) multi-residue method [1,22,23] to analyse fluopyram and tebuconazole in watermelon and soil by GC-MS was established.…”