Influences of acute ethanol exposure on locomotor activities of zebrafish larvae under different illumination

Guo, Ning; Lin, Jia Wei; Peng, Xiaoqian; Chen, Haojun; Zhang, Yinglan; Liu, Xiuyun; Li, Qiang

doi:10.1016/j.alcohol.2015.08.003

Cited by 26 publications

(12 citation statements)

References 34 publications

(42 reference statements)

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“…Together, the two studies conclusively demonstrate 1,2-propanediol-induced hyperactive swimming during the light phases. It is unclear how exposure to 1,2-propanediol impacts swimming activity; however, it has been previously shown that exposure of zebrafish to ethanol (0.1-1%) increases swimming activity during dark phases, while 2.0% ethanol increases activity in both light and dark phases, and that these changes are associated with changes in neurotransmitters involved with norepinephrine, dopamine, and serotonin pathways (Guo et al, 2015). Whether 1,2-propanediol and ethanol act through similar mechanisms to induce hyperactivity in larval zebrafish is unknown, but 1,2-propanediol has been reported to have 'alcohol-like' effects in humans (Fowles et al, 2013), suggesting that ethanol and 1,2-propanediol may have similar effects on the central nervous system (CNS).…”

Section: Discussionmentioning

confidence: 99%

Neurobehavioral effects of 1,2-propanediol in zebrafish (Danio rerio)

et al. 2018

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The use of electronic cigarettes (e-cigarettes) is increasing despite insufficient information concerning their long-term effects, including the effects of maternal e-cigarette use on pre- and postnatal development. Our previous study demonstrated that developmental exposure to 1,2-propanediol (a principal component of e-cigarette liquid) affected early development of zebrafish, causing reduced growth, deformities, and hyperactive swimming behavior in larvae. The current study extends assessment of the developmental toxicity of 1,2-propanediol by examining additional long-term behavioral effects. We demonstrate that embryonic/larval exposure of zebrafish to 1,2-propanediol (0.625% or 1.25%) not only affected behavioral parameters in the larvae, but also caused persisting behavioral effects in adults after early developmental exposure. Additional parameters, including neural and vascular development in larvae, stress response in adults, and concentration of neurotransmitters dopamine and serotonin in adult brain were examined, in order to explain the behavioral differences. These additional assessments did not find 1,2-propanediol exposure to significantly affect Tg(Neurog1:GFP) or the transcript abundance of neural genes (Neurog1, Ascl1a, Elavl3, and Lef1). Vascular development was not found to be affected by 1,2-propanediol exposure, as inferred from experiments with Tg(Flk1:eGFP) zebrafish; however, transcript abundance of vascular genes (Flk1, Vegf, Tie-2, and Angpt1) was decreased. No statistically significant changes were noted for plasma cortisol or brain neurotransmitters in adult fish. Lastly, analysis of gene transcripts involved with 1,2-propanediol metabolism (Adh5, Aldh2.1, and Ldha) showed an increase in Adh5 transcript. This is the first study to demonstrate that developmental exposure to 1,2-propanediol has long-term neurobehavioral consequences in adult zebrafish, showing that e-cigarettes contain substances potentially harmful to neurodevelopment.

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Section: Discussionmentioning

confidence: 99%

Neurobehavioral effects of 1,2-propanediol in zebrafish (Danio rerio)

et al. 2018

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“…Ethanol (alcohol or ethyl alcohol) is one of the most widely abused drugs consumed in the world [ 5 ] and therefore its toxic and psychoactive effect has been thoroughly studied. Zebrafish ( Danio rerio ) has been proposed as an ideal vertebrate model to investigate the neurobehavioural effects of ethanol addiction [ 6 ] as well as the mechanisms underlying those effects [ 7 – 9 ] mostly because ethanol metabolism in zebrafish liver is similar to humans [ 10 ]. In addition, zebrafish can be easily kept and bred in the laboratory, providing readily access at the embryonic and larval stages for genetic and screening tests [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Ethanol toxicity differs depending on the time of day

et al. 2018

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Ethanol is one of the most commonly abused drugs and consequently its toxic and psychoactive effect has been widely investigated, although little is known about the time-dependent effects of this drug. In the present research zebrafish was used to assess daily rhythms in ethanol toxicity and behavioural effects, as well as the temporal pattern of expression of key genes involved in ethanol detoxification in the liver (adh8a, adh5, aldh2.1 and aldh2.2). Our results showed marked differences in the mortality rate of zebrafish larvae depending on the time of day of the exposure to 5% ethanol for 1h (82% and 6% mortality in the morning and at night, respectively). A significant daily rhythm was detected with the acrophase located at “zeitgeber” time (ZT) = 04:22 h. Behavioural tests exposing zebrafish to 1% ethanol provoked a major decrease in swimming activity (68–84.2% reduction) at ZT2, ZT6 and ZT10. In contrast, exposure at ZT18 stimulated swimming activity (27% increase). During the day fish moved towards the bottom of the tank during ethanol exposure, whereas at night zebrafish increased their activity levels right after the exposure to ethanol. Genes involved in ethanol detoxification failed to show significant daily rhythms in LD, although all of them exhibited circadian regulation in constant darkness (DD) with acrophases in phase and located at the end of the subjective night. Taken altogether, this research revealed the importance of considering the time of day when designing and carrying out toxicological and behavioural tests to investigate the effects of ethanol, as the adverse effects of this drug were more marked when fish were exposed in the morning than at night.

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“…The stimulation of the PMR by isoproterenol observed in our study has been well documented 6 , 7 , indicating an involvement of β-adrenergic signalling pathway in triggering the PMR following photoreceptor stimulation. This is in-part supported by the innervation of the zebrafish hindbrain with noradrenergic neurons 23 . Furthermore, exposure to β-adrenergic receptor antagonists eliminates the PMR 6 , and co-exposure to the β-adrenergic receptor antagonist, propranolol, completely abolishes the stimulation of the PMR by isoproterenol (Gauthier and Vijayan, Unpublished).…”

Section: Discussionmentioning

confidence: 94%

Nonlinear mixed-modelling discriminates the effect of chemicals and their mixtures on zebrafish behavior

Gauthier

Vijayan

2018

Sci Rep

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Zebrafish (Danio rerio) early-life stage behavior has the potential for high-throughput screening of neurotoxic environmental contaminants. However, zebrafish embryo and larval behavioral assessments typically utilize linear analyses of mean activity that may not capture the complexity of the behavioral response. Here we tested the hypothesis that nonlinear mixed-modelling of zebrafish embryo and larval behavior provides a better assessment of the impact of chemicals and their mixtures. We demonstrate that zebrafish embryo photomotor responses (PMRs) and larval light/dark locomotor activities can be fit by asymmetric Lorentzian and Ricker-beta functions, respectively, which estimate the magnitude of activity (e.g., maximum and total activities) and temporal aspects (e.g., duration of the responses and its excitatory periods) characterizing early life-stage zebrafish behavior. We exposed zebrafish embryos and larvae to neuroactive chemicals, including isoproterenol, serotonin, and ethanol, as well as their mixtures, to assess the feasibility of using the nonlinear mixed-modelling to assess behavioral modulation. Exposure to chemicals led to distinct effects on specific behavioral characteristics, and interactive effects on temporal characteristics of the behavioral response that were overlooked by the linear analyses of mean activity. Overall, nonlinear mixed-modelling is a more comprehensive approach for screening the impact of chemicals and chemical mixtures on zebrafish behavior.

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Influences of acute ethanol exposure on locomotor activities of zebrafish larvae under different illumination

Cited by 26 publications

References 34 publications

Neurobehavioral effects of 1,2-propanediol in zebrafish (Danio rerio)

Neurobehavioral effects of 1,2-propanediol in zebrafish (Danio rerio)

Ethanol toxicity differs depending on the time of day

Nonlinear mixed-modelling discriminates the effect of chemicals and their mixtures on zebrafish behavior

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