Influence ofBordetella pertussis on the lymphatic tissue of mice

Finger, H; Mohr, W.; H, Hof; Elekes, E; Plager, L.

doi:10.1007/bf02122646

Cited by 2 publications

(3 citation statements)

References 32 publications

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“…[2] In all investigated organs and RBC, the ADA isozymes of nu/nu mice, Balb/c, and C3H mice have equal electrophoretic mobilities. [3] The nu/nu mouse cannot be used as a laboratory model to clear up the relation between ADA deficiency and CID.…”

Section: Discussionmentioning

confidence: 99%

“…No germinal centres are observed in lymph nodes. The white splenic pulpa is considerably reduced (3,7,19). There are only small numbers of Peyer's patches in the intestine.…”

mentioning

confidence: 99%

“…There are only small numbers of Peyer's patches in the intestine. They show no germinal centres and a depletion of lymphocytes in the thymus-dependent areas (3,19). The pathological anatomical phenomena and the immunodeficiency of the 'nude' mouse, compared with the above-mentioned cases of CID in man, led us to investigate the ADA in RBC and different organs of the nu/nu mouse and immunocompetent healthy Balb/c and C^H mice.…”

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confidence: 99%

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Adenosine Deaminase in the Congenitally Immunodeficient Nude Mouse

Körber

Hermann

1974

Scand J Immunol

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Various cases of human combined immunodeficiency together with adenosine‐deaminase deficiency have been described in the last 3 years. The congenitally thymus‐less (nu/nu) mouse could be presumed to serve as a possible laboratory model for examination of this combination of enzymatic deficiency and immunodeficiency. The present investigations, however, show similar isozyme pattern and enzymatic activity of nude mice and healthy Balb/c and C3H mice. Adenosine‐deaminase deficiency cannot be the cause of the immunodeficiency of the nude mouse.

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Section: Discussionmentioning

confidence: 99%

“…No germinal centres are observed in lymph nodes. The white splenic pulpa is considerably reduced (3,7,19). There are only small numbers of Peyer's patches in the intestine.…”

mentioning

confidence: 99%

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confidence: 99%

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Adenosine Deaminase in the Congenitally Immunodeficient Nude Mouse

Körber

Hermann

1974

Scand J Immunol

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Isolation and properties of the leukocytosis- and lymphocytosis-promoting factor of Bordetella pertussis.

Morse¹,

Morse²

1976

The Journal of Experimental Medicine

166

105

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In a previous report on the effects of the leukocytosis-and lymphocytosispromoting factor (LPF) 1 ofBordetella pertussis on cyclic adenosine 3':5'-monophosphate (cAMP) metabolism, a method for isolation of highly active LPF was presented briefly (1). In the present communication, the results of further studies on the isolation and chemical, immunochemical, and biological properties of LPF are described. It has been found that a single moiety is responsible for the induction in mice of leukocytosis with both lymphocytosis and granulocytosis, histamine sensitization, and hypoglycemia and unresponsiveness to the hyperglycemic effects of epinephrine. In confirmation of our preliminary report (2), LPF has been found to be clearly distinct from the hemagglutinating pili of B. pertussis, the properties of which are also described. Materials and MethodsB. pertussis Cultures. Strain NIH 114 (3779B), which has been utilized in continuing studies in this laboratory was also employed in the present investigations. Lyophilizod samples, obtained through the courtesy of Dr. Charles Manclark, Bureau of Biologics, Bethesda, Md., were reconstituted in sterile distilled H~O and plated on Bordet-Gengou medium (Clinical Sciences Inc., Whippany, N. J.). The 2-day growth at 36°C on one Petri dish was harvested in 10 ml of 2% casamino acids (technical grade; Difco Laboratories, Detroit, Mich.), and 1 ml of the suspension was inoculated into 100 ml of liquid medium in i liter Blake bottles. After 2 days of incubation at 36°C, 10 ml of the seed culture (approximately 5 × 10 x° organJma) was inoculated into 1 liter of liquid medium in Povitsky bottles which were then incubated at 35-36°C. In order to provide maximum surface area for aeration, all bottles were placed in the horizontal position during incubation. In the case of the 100 ml cultures, the depth of the medium was 1.0 cm and the surface area 218 cm2; the values for the 1-1iter cultures were 2.5 cm and 580 cm 2, respectively.The liquid medium was that previously described (3) except for the omission of an anion exchange resin which was found to be unnecessary for either optimal growth, maintenance of the Phase I state, or maximal production of LPF. Culture density was assessed with the National Institutes of Health opacity standard, and the criteria utilized to determine whether the organisms were Phase I included: a homogenous population of small, gram-negative coccobacilli and

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Influence ofBordetella pertussis on the lymphatic tissue of mice

Cited by 2 publications

References 32 publications

Adenosine Deaminase in the Congenitally Immunodeficient Nude Mouse

Adenosine Deaminase in the Congenitally Immunodeficient Nude Mouse

Isolation and properties of the leukocytosis- and lymphocytosis-promoting factor of Bordetella pertussis.

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