Influence of xenobiotics on rainbow trout liver estrogen receptor and vitellogenin gene expression

Abstract

Rainbow trout hepatocyte primary culture was used to test the influence of some xenobiotics on the expression of two genes implicated in reproduction, those for the estrogen implicated in reproduction, those for the estrogen receptor (ER) and vitellogenin (Vg). We showed that chlordecone, nonylphenol, a polychlorobiphenol (PCB) mixture (Aroclor 1245) and lindane were able to induce ER and Vg mRNA accumulation. Antiestrogens, 4-hydroxytamoxifen and ICI 164,384, prevented the effects of the xenobiotics, indicating that the induction of gene expression is mediated by the ER. Among these four xenobiotics, only chlordecone and nonylphenol were able to displace the binding of [3H]estradiol to ER-enriched COS-1 extracts, and to activate an estrogen-dependent reporter gene (ERE-TK-CAT) cotransfected with an expression vector containing ER cDNA. The results suggest that chlordecone and nonylphenol are direct inducers of rainbow trout ER and Vg gene expression, whereas PCBs and lindane act through their hepatic metabolites. Moreover, pentachlorophenol acts as an antagonist of the induction by estradiol of rainbow trout ER and Vg gene expression.

Exposure assessment for endocrine disruptors: some considerations in the design of studies.

“…Organochlorine compounds Chlordecone (Kepone) Pest control Gellert 1978;Hammond et al 1979;Soto et al 1994Soto et al , 1995Flouriot et al 1995Dieldrin Soto et al 1994Sonnenschein et al 1995;Arnold et al 1996Endosulfan Soto et al 1994Sonnenschein et al 1995;Arnold et al 1996α-Endosulfan Soto et al 1994β-Endosulfan Soto et al 1994, 1995 DDT (technical grades and isomers) Welch et al 1969;Bitman and Cecil 1970;Soto et al 1994, 1997Lindane Soto et al 1995Methoxychlor Tullner 1961Bitman and Cecil 1970;Bulger et al 1978;Cummings andMetcalf 1994, 1995;vom Saal et al 1995;Soto et al 1995Toxaphene Soto et al 1994Sonnenschein et al 1995;Arnold et al 1996 PCBs Dielectric fluids; contaminated soils; fish Bitman and Cecil 1970;Ecobichon and MacKenzie 1974;Gellert 1978;Jansen et al 1993 Phenolics Alkylphenol ethoxylates (APEs) Cleaning agents and surfactants White et al 1994;Sumpter and Jobling 1995;Jobling et al 1996;Routledge and Sumpter 1996 4-Alkylphenols Used in manufacture of APEs Soto et al 1991Soto et al , 1995Jobling and Sumpter 1993;White et al 1994;…”

Determination of Bisphenol A and 4-Nonylphenol in Human Milk Using Alkaline Digestion and Cleanup by Solid-Phase Extraction

“…Both compounds are of much concern because of their enormous amounts of usage and estrogenic activities. [1][2][3] BPA and NP are widely used in products to which humans are exposed. It is therefore of great importance to investigate their intake, bioavailability and metabolism in(to) humans.…”

The effects of prenatal PCBs on adult female paced mating reproductive behaviors in rats

“…PCBs and their metabolites can act at multiple nodes of the neuroendocrine axis: they may serve as hormone mimics (Connor et al, 1997), alter circulating hormone levels (Desaulniers et al, 1999), change patterns of estrous cyclicity (Meerts et al, 2004;Buitenhuis et al, 2004), disrupt hormone metabolism Kester et al, 2000;Yamane et al, 1975), influence endocrine-related and hypothalamic gene expression (Aluru et al, 2004;Bansal et al, 2005;Colciago et al, 2005;Flouriot et al, 1995;Gore et al, 2002;Pravettoni et al, 2005;Salama et al, 2003), interfere with hormone binding proteins (Brouwer and van den Berg, 1986;Chauhan et al, 2000), alter neuronal signaling to endocrine regions of the brain (Khan and Thomas, 2001;Morse et al, 1996;Seegal et al, 1985;Seegal et al, 1990) or indirectly affect steroid receptor availability via molecular crosstalk (Brunnberg et al, 2003;Pearce et al, 2004).…”