Influence of X-Ray and Microwave Radiation on Deamination of Purine Nucleotides in Yeast Cells Candida Guilliermondii Np-4

Abstract

In metabolism of living cells a key role play purine nucleotides which cells can be supplied either by de novo synthesis from lower molecular weight precursors, or by alternate ways of nucleotide synthesis or so-called "nucleotide salvage pathways", which allow reusing of intermediate products of nucleotide metabolism in nucleotide synthesis. This way is important in the post-stress repair period, saving energy and substrates in the repairing cells. Purine nucleotides are allosteric inhibitors of enzymes of nucleotide salvage pathways, therefore the increase in their catabolism leads to a decrease of their amount in the cells, which contributes to the intensive work of the nucleotide salvage pathways and provides substrates for DNA synthesis. Investigation of deamination of purine nucleotides in yeasts Candida guilliermondii NP-4 irradiated with X-rays, millimeter and decimeter electromagnetic waves, as well as after post-radiation incubation of cells has been realized. It has been shown that under the influence of X-ray and microwave irradiation in yeasts, the intensity of deamination of purine nucleotide-polyphosphates - ADP, ATP, GDF and GTP, has changed, which in all probability is an adaptive mechanism in the repair of yeasts after irradiation, provides the work of nucleotide salvage pathways, and can be associated with the metabolism of these compounds.

Uric acid as a target for the action of electromagnetic radiation

“…ЭМИ дециметрового диапазона способно оказывать повреждающее действие на ядерные структуры различных тканей и органов [18,19], усиливать катаболизм пуриновых оснований [20], что способствует увеличению продукции МК и как следствие интенсификации образования пероксидных радикалов. Повышение уровня МК является ведущим фактором в прогрессировании хронической болезни почек [13], маркером повышенного риска сердечно-сосудистых заболеваний [21,22].…”