Article Highlights• Kinetics of saccharose fermentation by Kombucha was analysed • A saccharose concentration model was defined as a sigmoidal function • Reaction rates were calculated as first derivatives of Boltzmann's functions • Saccharose fermentation by Kombucha occurred according to complex kinetics Abstract The kinetics of saccharose fermentation by Kombucha is not yet well defined due to lack of knowledge of reaction mechanisms taking place during this process. In this study, the kinetics of saccharose fermentation by Kombucha was analysed using the suggested empirical model. The data were obtained on 1.5 g L -1 of black tea, with 66.47 g L -1 of saccharose and using 10 or 15% (V/V) of Kombucha. The total number of viable cells was as follows: approximately 5×10 5 of yeast cells per mL of the inoculum and approximately 2x10 6 of bacteria cells per mL of the inoculum. The samples were analysed after 0, 3, 4, 5, 6, 7 and 10 days. Their pH values and contents of saccharose, glucose, fructose, total acids and ethanol were determined. A saccharose concentration model was defined as a sigmoidal function at 22 and 30 °C, and with 10 and 15% (V/V) of inoculum quantity. The determination coefficients of the functions were very high (R 2 > 0.99). Reaction rates were calculated as first derivatives of Boltzmann's functions. No simple correlation between the rate of reaction and independent variables (temperature and inoculum concentration) was found. Analysis of the empirical model indicated that saccharose fermentation by Kombucha occurred according to very complex kinetics.The consortium of yeasts and acetic acid bacteria known as the Kombucha culture exhibits a metabolic activity on sweetened tea, under batch conditions, giving a pleasant sour beverage containing useful compounds such as some organic acids and certain vitamins. The activity of Kombucha on the traditional carbon source saccharose was investigated by several authors [1-6] and the main pathways of saccharose transformation were determined. It has been proven that the yeast cells are responsible for the extracellular enzymatic hydrolysis of saccharose into glucose and fructose, and transformation of glucose and fructose into ethanol and CO 2 , while acetic acid