Influence of well defined protein source on in vitro maturation of human oocyte: human follicular fluid versus human serum albumin

Jee, Byung Chul; Han, Sang Hoon; Moon, Jeong Hee; Suh, Chang Suk; Kim, Seok Hyun

doi:10.1016/j.fertnstert.2007.02.052

Cited by 19 publications

(26 citation statements)

References 25 publications

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“…Reducing yield of immature oocytes is an another important aspect in stimulated IVF cycles because they usually have lower maturation capacity and seldom yield transferable embryos 6. In clinical practice, improving developmental competence of these rescued oocytes in vitro would be useful for women who yield only immature oocytes or show total fertilization failure of in vivo matured oocytes in stimulated IVF cycles 8-12…”

Section: Introductionmentioning

confidence: 99%

Oocyte Maturity in Relation to Woman's Age inIn VitroFertilization Cycles Stimulated by Single Regimen

et al. 2012

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PurposeDuring stimulated in vitro fertilization (IVF) cycle, up to 30% of the recovered oocytes are immature ones which have poor fertilization capacity; however, the precise influencing factors are largely unknown. Here, we analyzed the association of oocyte immaturity with woman's age in IVF cycles stimulated by single regimen.Materials and MethodsA total of one-hundred ninety five IVF cycles stimulated by recombinant FSH and GnRH antagonist protocol between 2003 and 2009 were analyzed retrospectively. The mean age of women was 34.2±4.0 (26-45 years). After triggering by exogenous hCG, an ultrasound-guided retrieval of oocytes was performed 35-36 hours later. All clinical data were stratified by woman's age; group I: ≤30 (n=36), II: 31-35 (n=83), III: 36-40 (n=57), and IV: ≥41 (n=19).ResultsThe total retrieved oocytes, as well as immature oocytes, were significantly lower in group IV, however, the mean % of immature oocytes was significantly higher in group IV than other age groups. Oocyte immaturity tended to decrease as increasing age in women aged 40 years or less.ConclusionIn stimulated IVF cycle, much higher oocyte immaturity was noted in women aged 41 years or more.

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Section: Introductionmentioning

confidence: 99%

Oocyte Maturity in Relation to Woman's Age inIn VitroFertilization Cycles Stimulated by Single Regimen

et al. 2012

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“…About 5-7% of oocytes retrieved are immature at the germinal vesicle (GV) stage, which were required further IVM process [5]. In IVF cycles using GnRH antagonist, up to 15% of oocytes are recovered as immature [6]. Immature oocytes can be fertilized after IVM, but generally resulting lower pregnancy rate than those matured in vivo .…”

Section: Introductionmentioning

confidence: 99%

Oocyte maturity in repeated ovarian stimulation

Lee

Kim

Jee

et al. 2011

Clin Exp Reprod Med

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ObjectiveDuring stimulated IVF cycles, up to 15% of oocytes are recovered as immature. The purpose of this study was to investigate the trend of oocyte maturity in repeated ovarian stimulation for IVF.MethodsOne hundred forty-eight patients were selected who underwent two consecutive IVF cycles using same stimulation protocol during 2008 to 2010. Ovarian stimulation was performed with FSH and human menopausal gonadotropin and flexible GnRH antagonist protocol in both cycles. Oocyte maturity was assessed according to presence of germinal vesicle (GV) and the first polar body. Immature oocyte was defined as GV stage or metaphase I oocyte (GV breakdown with no visible polar body) and cultured up to 48 hours. If matured, they were fertilized with ICSI.ResultsPercentages of immature oocytes were 30.8% and 32.9% (p=0.466) and IVM rates of immature oocytes were 36.2% and 25.7% (p=0.077), respectively. A significant correlation was noted between percentage of immature oocytes in the two cycles (R=0.178, p=0.03). Women with >40% immaturity in both cycles (n=21) showed lower fertilization rate of in vivo matured oocytes (56.4% vs. 72.0%, p=0.005) and lower pregnancy rate (19.0% vs. 27.1%, p=0.454) after the second cycle when compared with women with <40% immaturity (n=70). In both groups, female age, number of total retrieved oocyte and embryos transferred were similar.ConclusionIn repeated ovarian stimulation cycles for IVF, the immature oocyte tended to be retrieved repetitively in consecutive IVF cycles.

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“…To maximize embryo number from each cycle the potential of IVM MI oocytes to contribute to the cohort of available embryos is of interest within the human clinical and NHP ART research settings (4, 5). However, the vast majority of research in the human indicates that IVM MI oocytes exhibit reduced fertilization (4, 6), embryo quality (7, 8) and pregnancy outcomes (9). …”

Section: Introductionmentioning

confidence: 99%

Primate model of metaphase I oocyte in vitro maturation and the effects of a novel glutathione donor on maturation, fertilization, and blastocyst development

Curnow

Ryan

Saunders

et al. 2011

Fertility and Sterility

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Objective-To assess the effect of glutathione ethyl ester (GSH-OEt) on the development of macaque MI oocytes as a model for human MI oocyte in vitro maturation. Design-Prospective cohort studySetting-Non-human primate ART program. Animal(s)-23Macaca fascicularis females aged 6.5-12.5 years Intervention(s)-Ovarian stimulation and maturation of MI oocytes in i) HTF, ii) mCMRL-1066, iii) mCMRL-1066+GSH-OEt 3mM or iv) mCMRL-1066+GSH-OEt 5mM. Oocytes were assessed for maturation after 4-6 h (early) and 18-20 h (late) of culture. Mature oocytes were inseminated or subjected to glutathione (GSH) assay. Zygotes were cultured to the blastocyst stage for total differential cell counts.Main Outcome Measure(s)-Oocyte maturation rate, GSH content, pronuclear formation and blastocyst development and cell number were compared between IVM treatment groups and sibling in vivo matured (IVO) MII oocytes.Result(s)-Compared to HTF, mCMRL-1066 supported higher rates of normal fertilization and blastocyst development in early but not late maturing MI-MII oocytes. 5 mM GSH-OEt significantly increased blastocyst total cell and ICM cell number in early MI-MII oocytes compared to IVO and IVM controls. GSH-OEt significantly increased oocyte GSH content and fertilization in late maturing oocytes but not blastocyst development.

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Influence of well defined protein source on in vitro maturation of human oocyte: human follicular fluid versus human serum albumin

Cited by 19 publications

References 25 publications

Oocyte Maturity in Relation to Woman's Age inIn VitroFertilization Cycles Stimulated by Single Regimen

Oocyte Maturity in Relation to Woman's Age inIn VitroFertilization Cycles Stimulated by Single Regimen

Oocyte maturity in repeated ovarian stimulation

Primate model of metaphase I oocyte in vitro maturation and the effects of a novel glutathione donor on maturation, fertilization, and blastocyst development

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