Influence of vitamin K2 on lipid precursors of inflammation and fatty acids pathway activities in HepG2 cells

Kołakowski, Adrian; Kurzyna, Piotr Franciszek; Bzdęga, Wiktor; Żywno, Hubert; Harasim-Symbor, Ewa; Chabowski, Adrian; Konstantynowicz-Nowicka, Karolina

doi:10.1016/j.ejcb.2021.151188

Cited by 6 publications

(5 citation statements)

References 32 publications

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“…High-fat diet mice had decreased levels of serum non-esterified fatty acids after K2 exposure, 42 in parallel with a reduction of TG serum levels, while in vitro studies determined an increase of TG and a marked elevation in diacylglycerol 20 : 4. 43 In agreement, the TG levels of zebrafish in this work were altered, demonstrating an increase in the bioactive K3 group, a reduction in the non-active K1 group, and both an increase and reduction for different TG species in the bioactive K2 group. A correlation between plasma levels of more complex lipids, as lysophosphatidylcholine, sphingomyelins and phosphatidylcholines was suggested with obesity, although the pathways are not yet completely understood.…”

Section: Discussionsupporting

confidence: 82%

Reduction of neutral lipid reservoirs, bioconversion and untargeted metabolomics reveal distinct roles for vitamin K isoforms on lipid metabolism

Silva,

Preto,

Vasconcelos

et al. 2024

Food Funct.

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Section: Discussionsupporting

confidence: 82%

Reduction of neutral lipid reservoirs, bioconversion and untargeted metabolomics reveal distinct roles for vitamin K isoforms on lipid metabolism

Silva,

Preto,

Vasconcelos

et al. 2024

Food Funct.

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“…In context, VK2 successfully restored the oxidative/antioxidative balance with a parallel suppression of inflammatory genetic expression in the brain of naturally aging rats [ 27 ], which gives further credence to the observations of the current study. Moreover, VK2 suppressed TNF-α in human lymphocytes, fibroblasts and astrocytes, where inflammation is induced by various agents [ 52 ].…”

Section: Discussionmentioning

confidence: 99%

Vitamin K2 (MK-7) Intercepts Keap-1/Nrf-2/HO-1 Pathway and Hinders Inflammatory/Apoptotic Signaling and Liver Aging in Naturally Aging Rat

et al. 2022

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Aging is a naturally occurring physiological process with a deleterious impact on various body organs and humans’ well-being. The aging population is increasing worldwide, which imposes the need for the exploration of nutritional options that can intercept the impact of the aging processed on various body organs. Vitamin K2 (VK2) is a fat-soluble vitamin with emerging evidence on its therapeutic merits. In the current study, natural aging induced a significant liver deterioration with a disrupted Keap-1/Nrf-2/HO-1 axis and increased COX-2, iNOS and TNF-α expression and apoptotic and fibrotic changes. VK2 administration, on the other hand, improved the biochemical indices of liver function (total protein, albumin, ALT and AST); the suppressed hepatic expression of Keap-1 and increased the hepatic expression of Nrf-2 with a parallel increase in the hepatic activity of HO-1. Subsequently, the liver content and hepatic expression of TNF-α, COX-2 and iNOS were significantly retracted. In context, the liver content and hepatic expression of the fibrotic biomarkers TGFβ and TIMP significantly retracted as well. Moreover, the TUNEL assay confirmed the retraction of liver apoptotic changes. Of notice, electron transmission microscope examination confirmed the preservation of mitochondrial functions and preservation of the ultra-microscopical structures. In conclusion, the VK2-mediated interception of aging-induced Keap-1/Nrf-2/HO-1 signaling suppressed the hepatic contents of inflammatory and fibrotic biomarkers, as well as apoptotic changes with preservation of the hepatic architectural and functional status. VK2 can be presumed to be an effective nutritional supplement to the aging population to spare the liver, amongst other body organs, against aging-induced deleterious injury.

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“…HepG2 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) enriched with 10% FBS and 100 IU/mL penicillin and 100 μg/mL streptomycin (Gibico, Thermo Fisher Scientific, San Jose, CA, USA) at 37 °C and 5% CO 2 . The PA solution was produced as previously described, albeit with a small change [ 27 ]. After being dissolved in 1 M NaOH and heated to 70 °C, 0.5% bovine serum albumin (BSA; Sigma-Aldrich, St. Louis, MO, USA) was immediately mixed with PA and diluted in DMEM.…”

Section: Methodsmentioning

confidence: 99%

Effects of Fish Oil, Lipid Mediators, Derived from Docosahexaenoic Acid, and Their Co-Treatment against Lipid Metabolism Dysfunction and Inflammation in HFD Mice and HepG2 Cells

Choi

et al. 2023

Nutrients

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Although fish oil (FO) and lipid mediators (LM) derived from polyunsaturated fatty acids can prevent obesity, their combined effects and cellular metabolism remain unclear. Therefore, this study aimed to examine the potential protective and metabolic effects of FO in combination with LM (a mixture of 17S-monohydroxy docosahexaenoic acid, resolvin D5, and protectin DX [3:47:50], derived from docosahexaenoic acid (DHA)) on palmitic acid (PA)-induced HepG2 cells and high-fat- diet (HFD)-induced C57BL/6J mice after 9-week treatment. Lipid metabolism disorders and inflammation induced by HFD and PA were substantially reduced after FO and LM treatment. Further, FO and LM treatments reduced lipid accumulation by increasing fatty acid oxidation via peroxisome proliferator-activated receptor α and carnitine-palmitoyl transferase 1 as well as by decreasing fatty acid synthesis via sterol regulatory element-binding protein-1c and fatty acid synthase. Finally, FO and LM treatment reduced inflammation by blocking the NF-κB signaling pathway. Importantly, the combination of FO and LM exhibited more robust efficacy against nonalcoholic fatty liver disease, suggesting that FO supplemented with LM is a beneficial dietary strategy for treating this disease.

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Influence of vitamin K2 on lipid precursors of inflammation and fatty acids pathway activities in HepG2 cells

Cited by 6 publications

References 32 publications

Reduction of neutral lipid reservoirs, bioconversion and untargeted metabolomics reveal distinct roles for vitamin K isoforms on lipid metabolism

Reduction of neutral lipid reservoirs, bioconversion and untargeted metabolomics reveal distinct roles for vitamin K isoforms on lipid metabolism

Vitamin K2 (MK-7) Intercepts Keap-1/Nrf-2/HO-1 Pathway and Hinders Inflammatory/Apoptotic Signaling and Liver Aging in Naturally Aging Rat

Effects of Fish Oil, Lipid Mediators, Derived from Docosahexaenoic Acid, and Their Co-Treatment against Lipid Metabolism Dysfunction and Inflammation in HFD Mice and HepG2 Cells

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