Influence of vitamin E on mast cell mediator release

Gueck, Thomas; Aschenbach, Jörg R.; Fuhrmann, Herbert

doi:10.1046/j.1365-3164.2002.00307.x

Cited by 23 publications

(18 citation statements)

References 24 publications

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“…α-T has been reported to influence a large and diverse number of pathways that could contribute to immunologically activated inflammatory processes. These include NF-κB [35,36] and cytokine [24,29,37] pathways, phospholipase and arachidonic pathways [38,39], mast cell activation [40,41] and, importantly, in the context of the present studies, cell-mediated immunity [26,42,43] and antibody production [44] including that of IgE [22]. Central to the pathogenesis of allergic inflammation are antigen-specific, memory T-cell responses and antigen-specific IgE responses [1].…”

Section: Allergic Inflammation and Vitamin Ementioning

confidence: 78%

Severe Vitamin E deficiency modulates airway allergic inflammatory responses in the murine asthma model

Lim¹,

Vasu²,

Valacchi³

et al. 2008

Free Radical Research

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Allergic asthma is a complex immunologically mediated disease associated with increased oxidative stress and altered antioxidant defenses. It was hypothesized that α-tocopherol (α-T) decreases oxidative stress and therefore its absence may influence allergic inflammatory process, a pathobiology known to be accompanied by oxidative stress. Therefore, selected parameters of allergic asthma sensitization and inflammation were evaluated following ovalbumin sensitization and re-challenge of α-T transfer protein (TTP) knock-out mice (TTP -/-) that have greatly reduced lung α-T levels (e.g. < 5%) compared to their litter mate controls (TTP +/+ ). Results showed that severe α-T deficiency result in a blunted lung expression of IL-5 mRNA and IL-5 protein and plasma IgE levels compared with TTP +/+ mice following immune sensitization and rechallenge, although lung lavage eosinophil levels were comparable in both genomic strains. It is concluded that the initial stimulation of immune responses by the TTP -/-mice were generally blunted compared to the TTP +/+ mice, thus diminishing some aspects of subsequent allergic inflammatory processes.

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Section: Allergic Inflammation and Vitamin Ementioning

confidence: 78%

Severe Vitamin E deficiency modulates airway allergic inflammatory responses in the murine asthma model

Lim¹,

Vasu²,

Valacchi³

et al. 2008

Free Radical Research

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“…To examine the impact of the coating on mast cell function, the ability of the C2 cells to degranulate following stimulation was investigated. Histamine release and the activity of the enzyme -hexosaminidase were analyzed by means of high-performance liquid chromatography (HPLC) (10) and spectrophotometry (11), respectively. The wasp venom peptide toxin mastoparan was used for stimulation (10).…”

mentioning

confidence: 99%

“…Histamine release and the activity of the enzyme -hexosaminidase were analyzed by means of high-performance liquid chromatography (HPLC) (10) and spectrophotometry (11), respectively. The wasp venom peptide toxin mastoparan was used for stimulation (10). There was no interfering effect of the coating on mast cell degranulation (Figure 1).…”

mentioning

confidence: 99%

High-Efficiency Transfection of Suspension Cell Lines

2012

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Transfection of suspension cells has proven to be very difficult using conventional methods. Here, we present a simple and time-saving new transfection protocol wherein cell culture plates coated with chicken egg white are seeded with suspension cells prior to transfection. Our results demonstrate that coupling egg white coatings with commercially available transfection reagents leads to high transfection efficiency with suspension cell lines including canine mastocytoma C2 and the human myeloid cell line HL-60. This new approach, which should prove applicable to a wide range of cell lines, solves a crucial problem for researchers working with suspension cells.

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“…In some of these diseases it can be assumed that mast cell signaling and proliferation are deregulated, and normalization could play a beneficial role. In a model for canine atopic dermatitis, vitamin E inhibited histamine, prostaglandin D2, and chymase release both in unstimulated and mastoparan-stimulated canine mastocytoma cells (C2), but the signaling pathways involved have not been resolved in detail (24).…”

mentioning

confidence: 99%

Inhibition of HMC-1 Mast Cell Proliferation by Vitamin E

Kempná

Reiter²,

Arock³

et al. 2004

Journal of Biological Chemistry

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The effects of four natural tocopherols on the proliferation and signaling pathways were examined in the human mastocytoma cell line (HMC-1). The four tocopherols inhibited HMC-1 cell proliferation with different potency (␦ > ␣ ‫؍‬ ␥ > ␤). Growth inhibition correlated with the reduction of PKB (protein kinase B) phosphorylation by the different tocopherols. The reduction of PKB phosphorylation led to a decrease of its activity, as judged from a parallel reduction of GSK␣/␤ phosphorylation. The translocation of PKB to the membrane, as a response to receptor stimulation by NGF␤, is also prevented by treatment with tocopherols. In the presence of PKC or PP2A inhibitors, the reduction of PKB phosphorylation by tocopherols was still observed, thus excluding the direct involvement of these enzymes. Other pathways, such as the Ras-stimulated ERK1/2 (extracellular signal responsive kinase) pathway, were not affected by tocopherol treatment. The tocopherols did not significantly change oxidative stress in HMC-1 cells, suggesting that the observed effects are not the result of a general reduction of oxidative stress. Thus, the tocopherols interfere with PKB phosphorylation and reduce proliferation of HMC-1 cells, possibly by modulating either phosphatidylinositol 3-kinase, a kinase phosphorylating PKB (PDK1/2), or a phosphatase that dephosphorylates it. Inhibition of proliferation and PKB signaling in HMC-1 cells by vitamin E suggests a role in preventing diseases with mast cell involvement, such as allergies, atherosclerosis, and tumorigenesis.

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Influence of vitamin E on mast cell mediator release

Cited by 23 publications

References 24 publications

Severe Vitamin E deficiency modulates airway allergic inflammatory responses in the murine asthma model

Severe Vitamin E deficiency modulates airway allergic inflammatory responses in the murine asthma model

High-Efficiency Transfection of Suspension Cell Lines

Inhibition of HMC-1 Mast Cell Proliferation by Vitamin E

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