Influence of Vitamin E Given Intraperitoneally to Prevent Peritoneal Adhesions in Rats

Abstract

The aim of the study was to evaluate the influence of vitamin E administered intraperitoneally on the prevention of peritoneal adhesion formation in rats on the basis of macroscopic and microscopic assessment of the adhesions. material and methods. Experimental studies were performed on 50 Sprague-Dawley male rats, which were randomly divided into 5 groups, 10 rats in a group. Experimental group I (EI) included 10 rats which had peritoneal adhesions provoked by scraping of the wall of cecum and parietal peritoneum followed by intraperitoneal administration of vitamin E in the dose of 100 mg/kg body weight. Experimental group II (EII) included 10 rats which had peritoneal adhesions provoked by surgery, without administration of vitamin E. Control Group I (CI) included 10 rats which had the abdominal cavity opened without provoking peritoneal adhesions, and vitamin E was administered. Control Group II (CII) included 10 rats which had peritoneal adhesions provoked by surgery, and then lipid based solution was administered intraperitoneally. Control Group III (CIII) included 10 rats which had the abdominal cavity only opened and closed. Groups EI, CI and CII were the subject of the drugs intraperitoneal re-injection in first, second and third day after surgery. The animals were killed during the 8 th postoperative day. Macroscopic examination of peritoneal adhesions using the classification reported by Nair was performed and samples for microscopic examination were excised. Results. In group EI peritoneal adhesions were formed in 60% rats (40% weak and 20% solid). In group EII peritoneal adhesions were found in all animals (30% weak and 70% solid). Reduction of the inflammatory response and less severe fibrosis were observed in animals with intraperitoneal administration of vitamin E. conclusion. In the study, vitamin E administered intraperitoneally to rats decreased the intensity and extensiveness of peritoneal adhesions, which was confirmed by macroscopic and microscopic examinations.

Perbedaan Kadar Plasminogen Activator Inhibitor-1 Cairan Peritoneum Tikus Wistar antara yang Diberi Vitamin E Topikal atau Ketorolak Intramuskular atau Simvastatin Oral atau Kombinasi Ketiganya pada Tikus Wistar yang Dilakukan Abrasi Ileum

“…12 Penelitian lain oleh Makaraska dkk, menyimpulkan bahwa pemberian vitamin E intraperitoneal menurunkan intensitas dan luasnya adhesi peritoneum baik dilihat secara makroskopis maupun mikroskopis. 13 Pemberian vitamin E intraperitoneal sebagai barrier mekanik efektif dalam menekan terjadinya adhesi dibandingkan diberikan secara intramuskular. 8 Hal ini juga dikatakan oleh Portilla dkk bahwa dengan pemberian vitamin E yang dilarutkan dalam olive oil yang diberikan secara intraperitoneal dapat menurunkan terbentuknya adhesi sampai 80%.…”