Influence of various growth parameters on fungal growth and volatile metabolite production by indoor molds

Polizzi, Viviana; Adams, An; Saeger, Sarah De; Peteghem, Carlos Van; Moretti, Antonio; Kimpe, Norbert De

doi:10.1016/j.scitotenv.2011.10.035

Cited by 61 publications

(42 citation statements)

References 56 publications

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“…De acordo com a literatura, compostos microbianos podem ser produzidos de forma exclusiva por determinados microrganismos em condições controladas. 9,17 Comparação dos compostos identificados em A. rigidiuscula com banco de dados de compostos voláteis microbianos 18 revelou que apenas dezesseis deles foram anteriormente descritos como compostos voláteis de fungos (3-metilbutan-1-ol, 2-metilbutan-1--ol, octano, etilbenzeno, acetato de 3-metilbutan-1-ol, acetato de 2-metilbutan-1-ol, 1,2,4-trimetilbenzeno, feniletanol, naftaleno, decanal, (E)-geranilacetona, γ-muuroleno, β-selineno, valenceno, epizonareno, α-muuroleno). Compostos como borneol, 2-metilisoborneol, decan-3-ona, isoborneol, 4-etilguaiacol, decanoato de etila e β-cariofileno foram relatados como COVs de origem bacteriana.…”

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Perfil dos componentes voláteis produzidos pelo fungo fitopatógeno Albonectria rigidiuscula em diferentes condições de cultivo

et al. 2017

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Recebido em 15/12/2016; aceito em 10/04/2017; publicado na web em 13/06/2017 VOLATILE COMPONENTS PROFILE PRODUCED BY THE PHYTOPATHOGENIC FUNGUS Albonectria rigidiuscula IN DIFFERENT CONDITIONS CULTURE. The VOCs produced by the Albonectria rigidiuscula fungus in different conditions were obtained by headspace-solid phase microextraction (HS-SPME) and analyzed by gas chromatography-mass spectrometry (GC-MS). Fourty-four VOCs were identified in different culture media and incubation periods. The highest production of VOCs occurred in solid rice medium and 14 days of incubation. The compounds 3-methylbutan-1-ol, 2-methylbutan-1-ol, γ-muurolene and nerolidol acetate were produced by A. rigidiuscula in all culture conditions. γ-Muurolene was identified as the major compound (40.78%, 26.24% and 27.04%) in PD medium and it was suggested as a chemical marker for this fungus. The use of multivariate data analysis (PCA) and (HCA) allowed the discrimination of the volatile chemical profiles according to the culture medium.

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Section: Figura 1 Gráfico De Pareto Dos Efeitos Da Temperatura (A) unclassified

Perfil dos componentes voláteis produzidos pelo fungo fitopatógeno Albonectria rigidiuscula em diferentes condições de cultivo

et al. 2017

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“…Relative humidity, temperature, substrate (growth medium), number of fungal spores inoculated and oxidation stress are the main factors influencing fungal growth, metabolism and MVOCs' production in a laboratory setting [20][21][22]. For example, López-Malo et al [23] studied the effect of incubation temperature (10-30˝C), pH (3.0-4.0) and vanillin concentration (350-1200 ppm) on the growth of A. flavus.…”

Section: Introductionmentioning

confidence: 99%

“…They indicated that 24˝C was the optimal temperature for aflatoxin production. Polizzi et al [21] studied the influence of various growth parameters on fungal growth and volatile metabolite production by indoor molds. They proved that the range of MVOCs and the quantities were larger on malt extract agar than on wallpaper and plasterboard.…”

Section: Introductionmentioning

confidence: 99%

Effects of Growth Parameters on the Analysis of Aspergillus flavus Volatile Metabolites

et al. 2016

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Aspergillus flavus produces dangerous secondary metabolites known as aflatoxins, which are toxic and carcinogenic, and their contamination of agricultural products results in health issues and economic hardships in the U.S. and around the world. Early identification of aflatoxigenic isolates of A. flavus is the key in the management of these fungi. An emerging detection method for specific fungi identification involves the analysis of microbial volatile organic compounds (MVOCs) released by the fungi. Complicating this approach is the understanding that many factors influence metabolic production, including growth parameters, such as growth media, temperature, spore counts and oxidation stress. In addition, analytical and data analysis methods can also influence the results. Several growth and analysis methods were evaluated and optimized in order to better understand the effect of the methods on fungi MVOC signatures. The results indicate that carboxen/polydimethylsiloxane (CAR/PDMS) has the best extraction efficiency for the MVOCs emitted by A. flavus. Both chemical defined agar (CDA) and chemical defined liquid (CDL) are suitable growth media for MVOC emission studies. The highest MVOC production was found at 30˝C. Log transformation was considered one of the best data pretreatment methods when analyzing MVOC data and resulted in the best principal component analysis (PCA) clustering in the experiments with different growth media. This study aims to elucidate fungal volatile organic compounds (VOCs) differences due to variations in growth parameters as a first step in the development of an analytical method for the monitoring of aflatoxigenic A. flavus contamination in crop storage facilities.

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“…It has recently been proposed that the detection of volatiles can be used for the diagnosis of pulmonary infections (2,3) and lung cancer (4,5). Several aspergilli, including A. flavus, A. ustus, and A. versicolor, have been identified within the scope of environmental studies, where room air was analyzed to detect fungal pollution in houses (6)(7)(8). 2-Pentylfuran (2-PF) was detected in the breath of patients with A. fumigatus infection (9).…”

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The Volatome of Aspergillus fumigatus

2014

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bEarly detection of invasive aspergillosis is absolutely required for efficient therapy of this fungal infection. The identification of fungal volatiles in patient breath can be an alternative for the detection of Aspergillus fumigatus that still remains problematic. In this work, we investigated the production of volatile organic compounds (VOCs) by A. fumigatus in vitro, and we show that volatile production depends on the nutritional environment. A. fumigatus produces a multiplicity of VOCs, predominantly terpenes and related compounds. The production of sesquiterpenoid compounds was found to be strongly induced by increased iron concentrations and certain drugs, i.e., pravastatin. Terpenes that were always detectable in large amounts were ␣-pinene, camphene, and limonene, as well as sesquiterpenes, identified as ␣-bergamotene and ␤-trans-bergamotene. Other substance classes that were found to be present in the volatome, such as 1-octen-3-ol, 3-octanone, and pyrazines, were found only under specific growth conditions. Drugs that interfere with the terpene biosynthesis pathway influenced the composition of the fungal volatome, and most notably, a block of sesquiterpene biosynthesis by the bisphosphonate alendronate fundamentally changed the VOC composition. Using deletion mutants, we also show that a terpene cyclase and a putative kaurene synthase are essential for the synthesis of volatile terpenes by A. fumigatus. The present analysis of in vitro volatile production by A. fumigatus suggests that VOCs may be used in the diagnosis of infections caused by this fungus.A spergillus fumigatus is an opportunistic fungal pathogen that causes life-threatening invasive pulmonary infections (invasive aspergillosis [IA]) among immunocompromised patients. A sensitive, rapid, and accurate diagnostic assay for invasive aspergillosis is required to successfully fight this fungal infection (1). It has recently been proposed that the detection of volatiles can be used for the diagnosis of pulmonary infections (2, 3) and lung cancer (4, 5). Several aspergilli, including A. flavus, A. ustus, and A. versicolor, have been identified within the scope of environmental studies, where room air was analyzed to detect fungal pollution in houses (6-8). 2-Pentylfuran (2-PF) was detected in the breath of patients with A. fumigatus infection (9). It was shown that A. fumigatus produces farnesene when grown in vitro (10), and the use of terpene volatiles for the detection of IA has recently been proposed (11). However, the spectrum of volatile organic compounds (VOCs) produced by A. fumigatus and their synthesis have been poorly described. In this work, we characterized the patterns of volatile terpenes produced in vitro by A. fumigatus during growth under saprophytic conditions. In addition, the molecular pathways responsible for the synthesis of terpenoid volatiles were defined. MATERIALS AND METHODS Strains.Aspergillus fumigatus strain FGSC A1163 (ϭ DAL ϭ CBS144.89) was used for wild type strain-based experiments. Gene deletions were obta...

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Influence of various growth parameters on fungal growth and volatile metabolite production by indoor molds

Cited by 61 publications

References 56 publications

Perfil dos componentes voláteis produzidos pelo fungo fitopatógeno Albonectria rigidiuscula em diferentes condições de cultivo

Perfil dos componentes voláteis produzidos pelo fungo fitopatógeno Albonectria rigidiuscula em diferentes condições de cultivo

Effects of Growth Parameters on the Analysis of Aspergillus flavus Volatile Metabolites

The Volatome of Aspergillus fumigatus

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