Influence of UV Irradiation on the Composition of Human Stratum Corneum Lipids

Wefers, Heribert; Melnik, Bodo C.; Flür, Martin.; Bluhm, C.; Lehmann, Percy; Plewig, Gerd

doi:10.1111/1523-1747.ep12476124

Cited by 58 publications

(47 citation statements)

References 19 publications

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“…It has previously been demonstrated that irradiation with suberythemal doses of either UVA or UVB yielded an increase in the amount of stratum corneum lipids, especially the ceramide fraction, implying that the increase in the stratum corneum lipids after UVB irradiation is indicative of a local change in the biosynthesis or degradation of these compounds 62 . Moreover, abnormal stratum corneum lipid profiles can be associated with defective permeability barrier function in common skin disease like eczema and psoriasis, both of which exhibit hyperproliferation, impaired epidermal differentiation, and barrier dysfunction 63 . Interestingly, in lesional psoriatic skin, the level of glucosylceramide-β-glucosidase was seen to be increased compared to non-lesional skin 17,[63][64][65][66] .…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, abnormal stratum corneum lipid profiles can be associated with defective permeability barrier function in common skin disease like eczema and psoriasis, both of which exhibit hyperproliferation, impaired epidermal differentiation, and barrier dysfunction 63 . Interestingly, in lesional psoriatic skin, the level of glucosylceramide-β-glucosidase was seen to be increased compared to non-lesional skin 17,[63][64][65][66] .…”

Section: Discussionmentioning

confidence: 99%

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Raman spectroscopic mapping for the analysis of solar radiation induced skin damage

Ali¹,

Bonnier²,

Ptasiński³

et al. 2013

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Raman spectroscopic mapping for the analysis of solar radiation induced skin damage

Ali¹,

Bonnier²,

Ptasiński³

et al. 2013

Analyst

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“…aSMase activity is also known to be involved in the apoptotic pathway initiated by TNF-a (36) and Fas ligand (37). In addition, aSMase deficient cells, Niemann-Pick lymphocytes, and thymocytes derived from aSMase-knockout mice do not undergo apoptosis in response to UV radiation (8). Consistent with these reports, our results showed that basal and UV-induced MMP-1 mRNA and protein expressions are significantly decreased by the aSMase inhibitor, D609, in cultured human dermal fibroblasts.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, UV-damaged skin was more resistant to damage than normal skin, indicating improvement of the barrier function through induction of the amount of all stratum corneum lipids (7). These effects may be related to an increase in the stratum corneum ceramides (8). Activation of SMase by UV may be rapidly generated ceramide from hydrolysis of sphingomyelin at the plasma membrane (9).…”

mentioning

confidence: 99%

Ceramide accelerates ultraviolet-induced MMP-1 expression through JAK1/STAT-1 pathway in cultured human dermal fibroblasts

Kim¹,

Kim

Lee

et al. 2008

Journal of Lipid Research

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Ultraviolet (UV) irradiation accelerates formation of ceramide through hydrolysis of sphingomyelin and de novo synthesis. Here, we investigated the effects of ceramide on UV-induced matrix metalloproteinase-1 (MMP-1) expression in human dermal fibroblasts. Our results showed that acidic-sphingomyelinase (aSMase) and MMP-1 mRNA expression were increased by UV irradiation. Treatment of D609 (aSMase inhibitor) decreased the level of basal and UVinduced MMP-1 expression. On the other hand, basal and UVinduced MMP-1 expression was increased through induction of intracellular ceramide by D-MAPP, a ceramidase inhibitor. Our results also showed that MMP-1 protein expression was dose-dependently increased by C 2 -ceramide or SMase treatment. The activation of ceramide pathway by C 2 -ceramide enhanced phosphorylation of signal transducer and activators of transcription-1 (STAT-1), whereas ceramide-induced MMP-1 expression was potently prevented by piceatannol; Janus kinase ( JAK1) inhibtor; and WHI-P131, a specific inhibitor of JAK3; but not by AG490, JAK 2 inhbitor, in human dermal fibroblasts. We also found that UV induced the phosphorylation of STAT-1, and UV-induced MMP-1 expression was significantly decreased by JAK1 inhibitor, piceatannol. Overall, we demonstrate that induction of intracellular ceramide by UV may activate MMP-1 gene expression via JAK1/STAT-1 pathway. Therefore, we suggest that targeted modulation of the ceramide signaling pathway may offer a novel therapeutic approach for inhibiting MMP-1 expression, which is a causing gene of skin aging. UV (UV) irradiation is a major cause of epidermal inflammation, immunosuppression, altered epidermal permeability barrier function, premature aging, dyspigmentation, and the development of nonmelanoma and melanoma skin cancers (1-4). In addition, UV significantly increases the amount of intracellular ceramide, which is a major component of skin lipid and a lipid second messenger (5, 6). In addition, UV-damaged skin was more resistant to damage than normal skin, indicating improvement of the barrier function through induction of the amount of all stratum corneum lipids (7). These effects may be related to an increase in the stratum corneum ceramides (8). Activation of SMase by UV may be rapidly generated ceramide from hydrolysis of sphingomyelin at the plasma membrane (9).Ceramide has a number of important physiological functions that regulate cellular homeostasis, such as cell proliferation, differentiation, and apoptosis (10-13). As an intermediate in sphingomyelin biosynthesis, ceramide plays a key role in the metabolism of molecules that comprise membranes (14, 15). In addition, membrane ceramides contribute to membrane structure by influencing membrane lipid ordering by affecting membrane porosity and by altering the permeability of cell membranes (15, 16). Ceramide activates downstream targets including ceramide activated protein kinase, stress-activated protein kinase/c-Jun N-terminal kinase ( JNK), protein kinase C, ceramide activated phosphatase,...

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“…5 ml of a staining mixture containing acetic acid/H 2 SO 4 /H 3 PO 4 /H 2 O 5:1:1:95 Table 1. Controllable variables found in the literature playing a role in the outcome of human skin lipid profiles [10] Person linked Age [4], sex [11], phototype [12], interindividual variation [13] Environmental factors Seasonal variation [14], UV irradiation [15] Methodology Sampling body site [16], sampling skin depth [17], sample contamination [18] Extraction procedure [19], extraction efficiency [20], expression of results [8] Solvents for lipid separation [21], analytical technique for lipid determination [22] (v/v/v/v) and 0.5% CuSO 4 , after which the lipids were charred by heating the plates at 160°C for 10 min.…”

Section: Lipid Separationmentioning

confidence: 99%

Analysis of Epidermal Lipids of the Healthy Human Skin: Factors Affecting the Design of a Control Population

Paepe¹,

Weerheim

Houben³

et al. 2003

Skin Pharmacol Physiol

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The intervariability of studies on the lipids of human epidermis and stratum corneum is high because of the different origin of the skin samples and the variety of extraction methods used. In the present work, a high-performance thin-layer chromatographic technique has been used to study the parameters age, sex, and anatomical site for their effects on the lipid profiles recovered from healthy epidermal skin biopsy specimens. It was found that sex-related differences were seen at the level of the total ceramide concentration. Observed decreases in lipid concentration, due to ageing, depended on the anatomical site. Therefore, these variables should be controlled in a reproducible and standardized way in order to be able to study the direct relationship between skin condition and barrier lipid composition. Only when this relation is established, results of topical treatment can be scientifically evaluated.

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Influence of UV Irradiation on the Composition of Human Stratum Corneum Lipids

Cited by 58 publications

References 19 publications

Raman spectroscopic mapping for the analysis of solar radiation induced skin damage

Raman spectroscopic mapping for the analysis of solar radiation induced skin damage

Ceramide accelerates ultraviolet-induced MMP-1 expression through JAK1/STAT-1 pathway in cultured human dermal fibroblasts

Analysis of Epidermal Lipids of the Healthy Human Skin: Factors Affecting the Design of a Control Population

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