Influence of UV-B radiation on membrane lipid composition and ethylene evolution in 'Doyenne d'Hiver' pear shoots grown in vitro under different photosynthetic photon fluxes

Predieri, S.; Norman, H.D.; Krizek, Donald T.; Pillai, P. Shalini; Mirecki, Roman M.; Zimmerman, R. H.

doi:10.1016/0098-8472(95)00003-2

Cited by 82 publications

(52 citation statements)

References 33 publications

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“…A combination of sUV-B and heavy metal (Cd/Ni) produced more LPO as compared to individual treatments of either stress. The effect of UV-B on the membrane is related to the oxidation of the membrane components by free radicals (Predieri et al, 1995). Similar responses were observed in crop plants for various heavy metals (Somashekaraiah et al, 1992).…”

Section: Discussionsupporting

confidence: 56%

Interactive effects between supplemental ultraviolet-B radiation and heavy metals on the growth and biochemical characteristics of Spinacia oleracea L.

Mishra

Agrawal

2006

Braz. J. Plant Physiol.

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The impact of supplemental UV-B (sUV-B) radiation and heavy metals: Cd (68 µmol.kg -1 soil) and Ni (68 μmol.kg -1 soil) singly and in combination was studied on photosynthetic and non-photosynthetic pigments, metabolites, enzyme activities, biomass accumulation and the net primary productivity of spinach (Spinacia oleracea L. var. All Green), a common leafy vegetable plant. Both the stresses individually and in combination resulted in the reduction of photosynthetic pigments, ascorbic acid, and catalase activity, whereas a reverse trend was observed for anthocyanin, flavonoids and proline content, lipid peroxidation and peroxidase activity. The interactive effects of both the stresses were, however, less than additive. Combined treatment of sUV-B + Cd was more deleterious as compared to sUV-B + Ni and other individual treatments. Key words: Spinaicia oleracea, biomass, cadmium, enzymes, nickel, pigments, ultraviolet-B.Efeitos interativos entre radiação ultravioleta B suplementar e metais pesados no crescimento e características bioquí-micas de Spinacia oleracea L.: Estudou-se o impacto de radiação UV-B suplementar (sUV-B) e dos metais pesados Cd (68 µmol.kg -1 solo) e Ni (68 μmol.kg -1 solo), isoladamente ou em combinação, sobre pigmentos fotossintéticos e não fotossintéti-cos, metabólitos, atividades enzimáticas, acúmulo de biomassa e produtividade primária de espinafre (Spinacia oleracea L. var. All Green). Ambos estresses, individualmente ou em combinação, resultaram na redução dos pigmentos fotossintéticos, áci-do ascórbico, e atividade de catalase, sendo que uma tendência oposta foi observada para o conteúdo de antocianina, flavonói-des e prolina, peroxidação lipídica e atividade de peroxidase. Os efeitos interativos de ambos estresses foram, no entanto, menos que se fossem aditivos. O tratamento combinado de sUV-B + Cd foi o mais deletério quando comparado com sUV-B + Ni e outros tratamentos individuais.

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Section: Discussionsupporting

confidence: 56%

Interactive effects between supplemental ultraviolet-B radiation and heavy metals on the growth and biochemical characteristics of Spinacia oleracea L.

Mishra

Agrawal

2006

Braz. J. Plant Physiol.

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“…increases in synthesis of UVACs ), or differences in structure and composition of the thylakoid membranes (Predieri et al 1995). While little research has investigated the response of thylakoid membrane lipids to UV-B stress, there is some evidence that suggests lipid molecules are also important sites of UV-B damage (Kramer et al 1991;Predieri et al 1995). Thylakoid lipids, which have a high proportion of polyunsaturated fatty acids (Gounaris et al 1986) Abbreviations: UV-B, ultra-violet-B radiation; UV-A, ultra-violet-A radiation; UV-B BE , biologically effective UV-B radiation; PAR, photosynthetically active radiation; PS2, photosystem 2; PI, plastochron index; UVACs, ultraviolet absorbing compounds; Fo, initial fluorescence; Fv, variable fluorescence; Fm, maximum fluorescence; Fv/Fm, photochemical efficiency; Φ e , quantum yield of electron transport; Q P , photochemical quenching; Q NP , non-photochemical quenching; Chl, chlorophyll; TBARS, thiobarbituric acid reactive substances; MDA, malondialdehyde Can.…”

Section: Mots Clés: Rayonnement Uv-b Fluorescence Chlorophyllienne mentioning

confidence: 99%

“…Plants vary in sensitivity to UV-B radiation which may reflect tissue-level adaptations, e.g. increases in synthesis of UVACs ), or differences in structure and composition of the thylakoid membranes (Predieri et al 1995). While little research has investigated the response of thylakoid membrane lipids to UV-B stress, there is some evidence that suggests lipid molecules are also important sites of UV-B damage (Kramer et al 1991;Predieri et al 1995).…”

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confidence: 99%

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confidence: 99%

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Photosynthetic function, lipid peroxidation, and α-tocopherol content in spinach leaves during exposure to UV-B radiation

DeLong¹,

Steffen²

1997

Can. J. Plant Sci.

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. 1997. Photosynthetic function, lipid peroxidation and ␣-tocopherol content in spinach leaves during exposure to UV-B radiation. Can. J. Plant Sci. 77: [453][454][455][456][457][458][459]. Spinach (Spinacia oleracea L. 'Meridian') plants were irradiated for 9 h d -1 for 12 d with ultra-violet-B (UV-B) radiation [13.5 kJ m -2 d -1 of biologically effective UV-B radiation (UV-B BE ) + 350 µmol m -2 s -1 of photosynthetically active radiation (PAR)] or PAR (350 µmol m -2 s -1 ) to determine if incipient UV-B-induced inhibition of photosynthetic function occurred in photosystem 2 (PS2) reaction centers or in the lipid matrix of thylakoid membranes. Chlorophyll concentrations (leaf area basis) in UV-B-irradiated spinach leaves were significantly lower than in PAR plants after 4, 8 and 12 d of exposure. A significant UV-B-induced rise in initial fluorescence (Fo) at days 8 and 10 was accompanied by a decline in the photochemical efficiency (Fv/Fm) ratio, indicating a reduction in the efficiency of PS2 energy harvesting and trapping. When based upon the chlorophyll content of thylakoid membranes, UV-B radiation did not alter lipid peroxidation but did increase α-tocopherol content compared with the PAR treatment during the first 8 of the 12 exposure days. These data suggest that incipient UV-B damage to the photochemical apparatus did not occur in PS2 reaction center complexes nor in the lipid matrix of the thylakoid membranes. Photosynthetic function was more susceptible to UV-B-induced impairment than thylakoid membrane lipids were to UV-B radiation damage. 'Meridian') ont été exposés 9 h par jour pendant 12 jours à un rayonnement photosynthétiquement utile (RPU) de 350 µmol -2 s -1 ou à ce même rayonnement RPU + 13,5 kJ m -2 j -1 de rayonnement UV-B biologiquement effectif (UV-B BE ). L'objet était de déter-miner si un début d'inhibition de fonction photosynthétique par les UV-B se produi dans les centres de réaction du PS2 ou dans les lipides de la membrane des thylakoïdes. Après 4, 8 et 12 jours d'exposition, les concentrations de chlorophylle (par unité de surface foliaire) étaient significativement plus basses dans les plantes irradiées par les UV-B que dans celles recevant seulement le RPU. La hausse significative de la fluorescence initiale (Fo) due au UV-B observé aux jours 8 et 10 était suivie d'une baisse du rapport d'efficience photochimique Fv/Fm, signe d'une réduction de l'efficacité de la récolte et de la capture de l'énergie dans le PS2. Calculé en fonction de la teneur en chlorophylle de la membrane des thylakoïdes, le rayonnement UV-B ne modifiait pas la peroxydation des lipides mais provoquait l'accroissement des teneurs en α-tocophérol par rapport au traitement RPU dans les 8 premiers jours d'exposition. Il découle de ces observations qu'il n'y a pas eu de début d'endommagement par les UV-B dans les centres de réaction du PS2, ni dans les lipides de la membrane des thylakoïdes. La fonction photosynthétique était plus sensible aux effets négatifs des UV-B que les lipides de la membrane des thylakoï...

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“…UVBR exposure has been demonstrated to stimulate the generation of reactive oxygen species that could be responsible for the effects of UVBR on cell membranes (Predieri et al, 1995). The connection between oxidative damage on the one hand, and antioxidant defense mechanisms on the other, has been described in both, animal and plant cells (Kingston-Smith & Foyer, 2000).…”

Section: Discussionmentioning

confidence: 99%

Non enzymatic antioxidant photoprotection against potential UVBR induced damage in an Antarctic diatom (Thalassiosira sp.)

Hernando

Malanga

Puntarulo

et al. 2011

lajar

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ABSTRACT. In January 1999, unialgal cultures of the diatom Thalassiosira sp., solate from natural phytoplankton assemblages from Potter Cove, Antarctica, were exposed to solar ultraviolet radiation (UVR, 280-400 nm) in order to study the long-term acclimation of this species. Ultraviolet radiation B (UVBR, 280-315 nm) inhibited the growth rate during the first and second days of exposure. No UVBR inhibition was observed on the third day. The initial content of α-tocopherol (13 pmol (10 4 cell)) showed a marked decrease during the exponential growth phase (4 pmol (10 4 cell -1 ) by day 3). The initial content of β-carotene (3 pmol (10 4 cell) -1 ) did not show significant differences over time in cells exposed to UVBR. Two mycosporine-like amino acids (MAAs) were identified: porphyra-334 and shinorine. Cellular concentrations of MAAs increased significantly on days 2 and 3, and exposure of the algae to UVBR significantly enhanced this value. The relative importance of MAAs concentration was significant (P < 0.05) in relation to the α-tocopherol content. A positive correlation was shown between cellular MAAs concentration and growth rate. Our results suggest that photoprotection against UV-induced damage is characterized by short-term consumption of α-tocopherol and longer-term synthesis of MAAs. The UVBR damage/repair ratio during long-term exposure involves the combined action of several endogenous factors within the cell, with MAAs synthesis being the most effective factor related to photoprotection. Keywords: β-carotene, α-tocopherol, growth rate, diatoms, MAAs, photoprotection, Antarctica.Fotoprotección antioxidante no-enzimática contra el potencial daño inducido por UVBR en una diatomea antártica (Thalassiosira sp.) RESUMEN. Durante enero 1999, cultivos unialgales de una diatomea Thalassiosira sp., aislada de poblaciones fitoplanctónicas naturales de Caleta Carlos Potter, Antártida, fueron expuestos a radiación solar ultravioleta (UVR, 280-400 nm) con el objetivo de estudiar su aclimatación a largo plazo. La radiación ultravioleta B (UVBR, 280-315 nm) inhibió la tasa de crecimiento durante el primer y segundo día de exposición. No se observó inhibición por UVBR durante el tercer día. El contenido inicial de α-tocopherol (13 pmol (10 4 cel) -1 ) mostró una marcada disminución durante la fase de crecimiento exponencial (4 pmol (10 4 cel) -1 al día 3). El contenido inicial de β-caroteno (3 pmol (10 4 cel)) no mostró diferencias significativas entre días en células expuestas a UVBR. Se identificaron dos aminoácidos parecidos a micosporina (MAAs), porfira-334 y shinorina. Las concentraciones celulares de MAAs mostraron aumentos significativos en los días 2 y 3, que resultaron mayores cuando las algas fueron expuestas a UVBR. La importancia relativa de la concentración de MAAs fue significativa (P < 0.05) en relación al contenido de α-tocopherol. Se encontró una relación positiva entre la concentración celular de MAAs y la tasa de crecimiento. Estos resultados sugieren que la fotoprotección contra el daño inducido...

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Influence of UV-B radiation on membrane lipid composition and ethylene evolution in 'Doyenne d'Hiver' pear shoots grown in vitro under different photosynthetic photon fluxes

Cited by 82 publications

References 33 publications

Interactive effects between supplemental ultraviolet-B radiation and heavy metals on the growth and biochemical characteristics of Spinacia oleracea L.

Interactive effects between supplemental ultraviolet-B radiation and heavy metals on the growth and biochemical characteristics of Spinacia oleracea L.

Photosynthetic function, lipid peroxidation, and α-tocopherol content in spinach leaves during exposure to UV-B radiation

Non enzymatic antioxidant photoprotection against potential UVBR induced damage in an Antarctic diatom (Thalassiosira sp.)

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