Influence of use N-acetylcystein for the correction of antioxidant status
                        in rabbits’ on indicators of blood plasma lipid profile

,; Koshevoy, V.I.; Vikulina, G.V.; Naumenko, S.V.

doi:10.31073/vet_biotech43-08

Cited by 2 publications

(2 citation statements)

References 21 publications

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“…Similar changes were found in paracetamol-induced rats, with serum triacylglycerols, LDL-cholesterol and VLDLcholesterol levels showing significant increases, while HDLcholesterol levels showed a significant decrease compared to control rats (Almajwal & Elsadek, 2015), also for APAP-induced hepatotoxicity in mice -in addition, isorhamnetin treatment significantly modulated lipid profiles (triacylglycerols, LDL-cholesterol, and HDL-cholesterol levels) that were altered in response to APAP administration (P < 0,05) (Gungor et al, 2023). It is possible to correct changes in lipid metabolism and redox status in the body of rabbits by introducing N-acetylcysteine, which was shown by us in a previous study (Koshevoy et al, 2023).…”

Section: Fig 1 Dynamics Of the Content Of Biomarkers Of Oxidative Str...mentioning

confidence: 56%

Plasma lipid profile and sex hormone levels in rabbits under paraceta-mol-induced oxidative stress

Vikulina,

Koshevoy,

Naumenko

et al. 2024

Ukr. Jour. of Vet. and Agr. Sci.

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Changes in the redox balance at different levels, which lead to the accumulation of toxic radicals against the background of a decrease in the content of redox-active compounds, i.e., a state of oxidative stress (OS), are considered the leading pathogenetic factor in pathological conditions. Processes of peroxide oxidation of biological substrates, especially cell membrane lipids and lipophilic compounds have both regulatory and pathological effects in the body of animals and humans. For now, it remains relevant to establish the regularities of interaction of metabolism during OS development. Among the large number of existing OS models, attention is drawn to those that can be reproduced in clinical practice, as a complication of treatment measures or an error in the medical strategy, due to a constant influence on peroxidation processes in the body. Important among such means is paracetamol (acetaminophen, APAP) – one of the most common antipyretic and analgesic drugs. In addition to the known hepato-, nephro- and neurotoxic effects of its long-term and/or excessive use, there are data on reproductive toxicity and disorders of lipid metabolism. Therefore, the aim of our work was to determine the lipid profile and the level of sex hormones in male rabbits during paracetamol-induced OS. The study was performed on male rabbits of the Khila breed. According to the principle of analogs, a control group (n = 12) was formed, which were kept on a standard diet, and an experimental group (n = 12), whose animals were simulated the state of OS by oral injection of a solution of paracetamol with food at a dose of 300 mg/kg of weight body once during 21 days. The following results were obtained by determining the dynamics of changes in biochemical indicators in the rabbit body during chronic injection of paracetamol: in the rabbits of the experimental group, after the injection of paracetamol, the level of diene conjugates was 63.0 % higher, and the content of thiobarbiturate-active products was 1.26 times higher (P ˂ 0.05). A decrease in the activity of the antioxidant defence system was also noted – the amount of reduced glutathione decreased by 35.4 %, and the activity of superoxide dismutase decreased by 25.6 % (P ˂ 0.05). The obtained changes were consistent with the comprehensive indicator of total antioxidant activity, which on the 21st day of the experiment was 34.1 % lower than the values of the control group (P ˂ 0.05). At the same time, the level of steroid hormones also changed during the study – the level of total testosterone gradually decreased (on the 21st day – by 22.8 %, and at the end of the experiment – by 30.9 %, P ˂ 0.05), while the level of 17β-estradiol had an upward trend and on the 21st day was higher than the control indicators by 10.7 % (P ˂ 0.05). Significant changes in lipid metabolism were found in male rabbits – the total cholesterol content on the 21st day of the experiment exceeded the data of control animals by 25.3 %, the level of triacylglycerols – by 42.3 %, and the content of low-density lipoprotein cholesterol also increased by 1.1 times (P ˂ 0.05), and the high-density lipoprotein cholesterol level, on the contrary, was reduced by 17.0 % (P ˂ 0.05). In general, the obtained results indicate the relationship between the development of OS with changes in lipid metabolism and the balance of sex hormones, which allows us to use the model of paracetamol-induced OS in rabbits for further research.

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Section: Fig 1 Dynamics Of the Content Of Biomarkers Of Oxidative Str...mentioning

confidence: 56%

Plasma lipid profile and sex hormone levels in rabbits under paraceta-mol-induced oxidative stress

Vikulina,

Koshevoy,

Naumenko

et al. 2024

Ukr. Jour. of Vet. and Agr. Sci.

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“…Проблемою сучасного вирощування м'яса кролів у світі є застосування в раціоні нових добавок, що негативно впливають на гематологічні показники та організм тварин загалом, внаслідок чого вивчення гомеостазу у кролів залежно від компонентів раціону може допомогти у коригуванні деяких поживних речовин у раціоні (Lesyk et al, 2020;Boiko et al, 2020b). Великий обсяг наукових досліджень щодо комбінованого застосування кормових добавок органічного походження з гормональними засобами індукції овуляції та стимуляції охоти мають обмежену кількість даних щодо безпечності останніх за тривалого застосування (Naumenko & Koshevoy, 2017;Salem et al, 2020;Koshevoy et al, 2023).…”

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Morphological assessment of ovaries during stimulation of ovulation in rab-bits with equine serum gonadotropin in different doses

Tverdokhlib

2023

SMLNUVMB

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Ovulatory stimulation of rabbits during artificial insemination requires the use of hormonal drugs to stimulate maturation of follicles, effective fertilization and full manifestation of female sexual function. For this purpose, gonadotropins or analogues of gonadotropin-releasing hormone are common in the practice of rabbit breeding. The latter have a selective effect on folliculogenesis and, under certain conditions, are not capable of inducing ovulation in rabbits of “zero” reproductive cycle, that is, those that have not given birth. Instead, gonadotropins, in particular serum gonadotropin obtained from foal mares (eCG), have a pronounced effect on folliculogenesis in animals. eCG is a glycoprotein hormone secreted by trophoblastic epithelial cells of fetal origin. In other species, except for horses, it is able to induce the release of both luteinizing and follicle-stimulating hormone. That is why eCG is widely used together with human chorionic gonadotropin to induce follicle growth and ovulation. However, there are data on complications and pathological conditions that may occur with long-term use of eCG, especially in genital tract. Therefore, the goal of our research was the morphological evaluation of the functional compartments of ovaries when using eCG to stimulate reproductive function of rabbits. Schemes of hormonal treatment of females in the conditions of a private rabbit farm, an experiment was conducted, which included the introduction of eCG in different doses (40 IU for animals of experimental group 1 and 25 IU for experimental group 2) during five reproductive cycles. Selection of material for histological studies, its fixation, production and analysis of tissue sections stained with hematoxylin and eosin were performed according to commonly used methods. The complex of morphofunctional changes in the ovaries of the rabbits of experimental groups corresponds to the pathomorphological picture of the syndrome of hyperstimulated ovaries. In our experiment, obtained changes were more pronounced at a dose of 40 IU, using which we additionally detected signs of increased immunoreactivity in body of rabbits, as evidenced by the presence of nodular lymphoid formations in outer theca of secondary and tertiary follicles. Thus, as a safe regimen for the use of eCG for ovulatory stimulation in rabbits, we recommend a dosage of 25 IU for 1–2 reproductive cycles.

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Influence of use N-acetylcystein for the correction of antioxidant status in rabbits’ on indicators of blood plasma lipid profile

Cited by 2 publications

References 21 publications

Plasma lipid profile and sex hormone levels in rabbits under paraceta-mol-induced oxidative stress

Plasma lipid profile and sex hormone levels in rabbits under paraceta-mol-induced oxidative stress

Morphological assessment of ovaries during stimulation of ovulation in rab-bits with equine serum gonadotropin in different doses

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