Influence of underfeeding on the spontaneous contraction and on metabolism of labelled arachidonic acid in uteri isolated from intact and ovariectomized rats

Casalino, S.; Linares, J.A.; Goldraij, A

doi:10.1016/s0952-3278(96)90091-5

Cited by 7 publications

(4 citation statements)

References 16 publications

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“…These data confirmed the capacity of PG to stimulate motility and glucose metabolism in the isolated uteri of rats. This stimulatory action was in agreement with previous findings of our laboratory in adult animals [14,15,17] and of other authors [16]. The data described herein showed that the isolated uterus converts [C 14 ] arachidonic acid to different eicosanoids: PgE 2 , PgF 2α , 6-ketoPgF 1α (indicating the production of PgI 2 ) and TXB 2 (indicating the production of TXA 2 ) in both groups of immature rats.…”

Section: Discussionsupporting

confidence: 93%

“…A modification of the method of Brendt and Snayder that measures the conversion of 14 C arginine into [C 14 ] citrulline was used. The method indirectly measures NO production, an index of NOS activity [11].…”

Section: Determination Of Nos Activitymentioning

confidence: 99%

“…The first group of experiment was carried out to analyse contractile activity and production of 14 CO 2 from [U 14 C] glucose in a KRB medium or in KRB medium with the addition of indomethacin (5 × 10 -6 M) alone or with prostaglandin (Pg): PgE 1 , PgE 2 or PgF 2α. Subsequently, we analysed the effect of Nω nitro-L-arginine methyl ester (NAME), 500 μM; sodium nitroprusside (SNP), 400 μM and L-arginine, benzethonium hydroxide, 1 mM (Sigma Chemical Co., St. Louis, Mo, USA) over 14 …”

Section: Drugsmentioning

confidence: 99%

“…Subsequently, we analysed the effect of Nω nitro-L-arginine methyl ester (NAME), 500 μM; sodium nitroprusside (SNP), 400 μM and L-arginine, benzethonium hydroxide, 1 mM (Sigma Chemical Co., St. Louis, Mo, USA) over 14 …”

Section: Drugsmentioning

confidence: 99%

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Glucose metabolism in isolated uteri of immature rats. Influence of prostaglandins and nitric oxide

2006

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-We studied the contractile activity and glucose metabolism, in terms of production of 14 CO 2 from [ 14 C] glucose, in isolated uteri of immature rats. Immaturity was due to age or exposure to a restricted diet. The contractile activity in both prepubertal groups persisted for a period of 60 minutes and fell when indomethacin was added to the KRB medium. The production of 14 CO 2 was greater than for adult rats and fell as a result of the addition of indomethacin. The metabolism of [ 14 C] arachidonic acid showed that the percentage of eicosanoids released in age related immature uteri was greater than that in restricted diet related immature uteri. In animals that are immature as a result of exposure to a restricted diet, 14 CO 2 fell due to the effect of NAME. Sodium nitroprusside and L-arginine increased the production of 14 CO 2 . This effect was reverted by NAME and indomethacin. Conversely, the uteri of age related prepubertal rats were not affected. The level of activity of nitric oxide synthase was higher in restricted diet related immature animals and fell following the addition of NS-398. We may conclude that in rats exposed to a restricted diet, NO and COX-2 participate in glucose metabolism whereas they would not be involved in age related prepubertal animals.immature rats / glucose metabolism / nitric oxide / arachidonic acid

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