Influence of triazole pesticides on tillage soil microbial populations and metabolic changes

Satapute, Praveen; Kamble, Milan V.; Adhikari, Shivakantkumar; Jogaiah, Sudisha

doi:10.1016/j.scitotenv.2018.10.099

“…Moreover, P. aeruginosa has also been documented for its efficacy in plant growth promotion and is a promisive biocontrol candidate 23 . This microorganism exhibits a versatile biological activity which can be utilized for solving many agricultural 20,21,23 and environmental issues 24 .…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of nutrient dependent pyocyanin from Pseudomonas aeruginosa and its dye and agrochemical properties

DeBritto

¹

,

Gajbar

²

,

Satapute

³

et al. 2020

Sci Rep

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Pyocyanin is a blue green phenazine pigment produced in large quantities by active cultures ofPseudomonas aeruginosa, with advantageous applications in medicine, agriculture and for the environment. Hence, in the present study, a potent bacterium was isolated from agricultural soil and was identified morphologically and by 16S rRNA sequencing as P. aeruginosa (isolate KU_BIO2). When the influence of nutrient supplements in both King's A and Nutrient media as amended was investigated, an enhanced pyocyanin production of 2.56 µg ml −1 was achieved in King's A medium amended with soya bean followed by 1.702 µg ml −1 of pyocyanin from the nutrient medium amended with sweet potato. Purified pyocyanin was characterized by UV-Vis Spectrophotometer and Fourier-Transform Infrared spectroscopy (FTIR). Furthermore, Liquid Chromatography Mass Spectrum (LCMS) and Nuclear Magnetic Resonance (NMR) confirmed its mass value at 211 and as N-CH 3 protons resonating at 3.363 ppm as a singlet respectively. The isolated pyocyanin displayed remarkable dye property by inducing color change in cotton cloth from white to pink. Lastly, the antifungal activity of test pyocyanin showed inhibition of growth of rice blast fungus, Magnaporthe grisea and bacterial blight of rice, Xanthomonas oryzae at concentrations of 150 and 200 ppm, respectively. Thus, this investigation provides evidence for diverse actions of pyocyanin which are nutrient dependent and are capable of acting on a large scale, by utilizing microbes existing in agriculture wastes, and thus could be used as an alternative source in the making of natural textile dyes with strong durability and a broad spectrum of ecofriendly agrochemicals.Microorganisms are biological agents which help to solve many problems related to health, agriculture and the environment 1-3 . Microorganisms have been the study at interest in recent years because of production of novel secondary metabolites 4 . These metabolites exhibit antimicrobial, antiviral or antitumor as well as anticoagulant properties, and the production of secondary metabolites may have evolved as an alternative strategy to switching off metabolic pathways by various control mechanisms 5-7 . Products of secondary metabolism, such as pigments, could also be of considerable selective advantage since they could eliminate possible microbial competitors 8 .Natural pigments have been obtained and used since long ago, but interest in them has decreased due to toxicity problems. Hence, pigments from microbial sources are good alternatives for various applications 9,10 . Pseudomonas aeruginosa is one of the most commercially valuable organisms, many of which are responsible for producing soluble pigments like pyocyanin (blue), pyoveridin (yellow-green), pyorubin (red) and pyomelanin (brown) 11 . P. aeruginosa produces pyocyanin (N-methyl-1-hydroxyphenazine) which is a water soluble

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“…The chemical and physical properties of all soil samples were analyzed. Soil pH was measured by a pH meter and soil electrical conductivity by an electric conductivity bridge, according to the manufacturers' protocols (Satapute et al, 2019). The moisture content of the soil was determined after dry weights were obtained by drying the samples at 105 • C. The soil organic matter content was measured using Yang's method (Yang et al, 2019).…”

Section: Soil Physicochemical Propertiesmentioning

confidence: 99%

Diversity of rhizosphere and endophytic fungi in Atractylodes macrocephala during continuous cropping

Zhu

¹

,

Wu

²

,

Ji³

et al. 2020

PeerJ

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Rhizospheric and endophytic fungi are key factors which influence plant fitness and soil fertility. Atractylodes macrocephala is one of the best-known perennial herbs used in traditional Chinese medicine. Continuous cropping has been shown to have a negative effect on its growth and renders it more susceptible to microbial pathogen attacks. In this study, we investigated the effects of continuous cropping on the endophytic and rhizospheric fungi associated with A. macrocephala using culture-independent Illumina MiSeq. Continuous cropping was found to decrease fungal diversity inside plant roots, stems, leaves and tubers. Additionally, we found that the structure and diversity of rhizospheric and endophytic fungal communities were altered by root-rot disease. Fusarium was overrepresented among root-rot rhizospheric and endophytic fungi, indicating that it has a major negative impact on plant health during A. macrocephala monocropping. Canonical correspondence analysis of the control and diseased samples revealed that pH, hydrolysis N, electrical conductivity and Hg content were well-correlated with fungal community composition during continuous cropping. Taken together, these results highlight the ecological significance of fungal communities in maintaining plant fitness and will guide the development strategies to attenuate the negative impacts of A. macrocephala continuous cropping.

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“…The chemical and physical properties of all soil samples were analyzed. Soil pH was measured by a pH meter and soil electrical conductivity by an electric conductivity bridge, according to the manufacturers' protocols (Satapute et al 2019). The moisture content of the soil was determined after dry weights were obtained by drying the samples at 105 °C.…”

Section: Soil Physicochemical Propertiesmentioning

confidence: 99%

Peer Review #2 of "Diversity of rhizosphere and endophytic fungi in Atractylodes macrocephala during continuous cropping (v0.1)"

2020

0

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Rhizospheric and endophytic fungi are key factors which influence plant fitness and soil fertility. Atractylodes macrocephala is one of the best-known perennial herbs used in traditional Chinese medicine. Continuous cropping has been shown to have a negative effect on its growth and renders it more susceptible to microbial pathogen attacks. In this study, we investigated the effects of continuous cropping on the endophytic and rhizospheric fungi associated with A. macrocephala using culture-independent Illumina MiSeq. Continuous cropping was found to decrease fungal diversity inside plant roots, stems, leaves and tubers. Additionally, we found that the structure and diversity of rhizospheric and endophytic fungal communities were altered by root-rot disease. Fusarium was overrepresented among root-rot rhizospheric and endophytic fungi, indicating that it has a major negative impact on plant health during A. macrocephala monocropping. Canonical correspondence analysis of the control and diseased samples revealed that pH, hydrolysis N, electrical conductivity and Hg content were well-correlated with fungal community composition during continuous cropping. Taken together, these results highlight the ecological significance of fungal communities in maintaining plant fitness and will guide the development strategies to attenuate the negative impacts of A. macrocephala continuous cropping.

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Influence of triazole pesticides on tillage soil microbial populations and metabolic changes

Cited by 104 publications

References 38 publications

Isolation and characterization of nutrient dependent pyocyanin from Pseudomonas aeruginosa and its dye and agrochemical properties

Isolation and characterization of nutrient dependent pyocyanin from Pseudomonas aeruginosa and its dye and agrochemical properties

Diversity of rhizosphere and endophytic fungi in Atractylodes macrocephala during continuous cropping

Peer Review #2 of "Diversity of rhizosphere and endophytic fungi in Atractylodes macrocephala during continuous cropping (v0.1)"

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