Influence of Treating Essential Oils of Canarium Tree Resin on The Level Malondialdehyde (MDA), Protease Activity, Bronchi Histopathology Description and Expression of Tumor Necrosis Alpha Factor (TNF-α) on Asthma Rats (Rattus norvegicus)

Abstract

Essential oils of canarium tree resin (Canarium indicum L) have anti-inflammatory and antioxidant activity. The purpose of this study was to investigate the potential of essential oils of canarium tree resin on the expression of TNF-α, changes in bronchial epithelial cell features, MDA levels and protease activity on asthma rats. This study used four groups of rats, the negative control group, asthma group, therapy group dose of 25 mg/kg body weight, 50 mg/kg body weight and 100 mg/kg body weight. The ashtma rats were prepared by OVA and LPS. TNF-α expression was calculated using Walter calculation, MDA level was measured using Thiobarbituric Acid (TBA), measurement of protease activity was done by spectrophotometric method, and all data analyzed by ANOVA. Histopathologic observation of bronchi was done using Olympus BX51 microscope. The results showed that the therapy of essential oils of canarium tree resin of 25 mg/kg body weight, 50 mg/kg body weight and 100 mg/kg body weight significantly (p<0.05) decreased, MDA up to 54%, protease activity up to 46%, improved bronchial epithelium and TNF-α expression up to 67%. This study proves that essential oils of canarium tree resin can be effectively used as therapy on asthma rats.