Influence of toluene and salinity on biosurfactant production by Bacillus sp.: scale up from flasks to a bench-scale bioreactor

Souza, Elias Costa de; Vessoni-Penna, Thereza Christina; Arni, Saleh Al; Domínguez, José Manuel; Converti, Attílio; Oliveira, Ricardo Pinheiro de Souza

doi:10.1590/0104-6632.20170342s20150787

Cited by 10 publications

(6 citation statements)

References 30 publications

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“…Distilled water offers free dissolved or suspended material that is also safe for the growth of desired microorganisms which allow them to produce the required product. For example, distilled water was used to dissolve all components for isolate S2 [ 174 ], P. putida , B. megatherium , B. licheniformis, and B. subtilis [ 175 ] to grow and to yield biosurfactant in laboratory scale. It does not affect the properties of the product generated by microorganisms due to its neutral properties.…”

Section: Media Components For Biosurfactant Productionmentioning

confidence: 99%

Culture Medium Development for Microbial-Derived Surfactants Production—An Overview

2018

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Surfactants are compounds that can reduce the surface tension between two different phases or the interfacial tension of the liquid between water and oil, possessing both hydrophilic and hydrophobic moieties. Biosurfactants have traits that have proven to be advantageous over synthetic surfactants, but these compounds do not compete economically with synthetic surfactants. Different alternatives increase the yield of biosurfactants; development of an economical production process and the usage of cheaper substrates during process have been employed. One of the solutions relies on the suitable formulation of a production medium by including alternative raw materials sourced from agro-wastes, hydrocarbons, or by-products of a process might help in boosting the biosurfactant production. Since the nutritional factors required will be different among microorganisms, the establishment of a suitable formulation for biosurfactant production will be challenging. The present review describes various nutrients and elements considered in the formulation of a production medium with an approach focusing on the macronutrient (carbon, nitrogen source, and C/N ratio), minerals, vitamins, metabolic regulators, and salinity levels which may aid in the study of biosurfactant production in the future.

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Section: Media Components For Biosurfactant Productionmentioning

confidence: 99%

Culture Medium Development for Microbial-Derived Surfactants Production—An Overview

2018

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“…In comparison with the chemical equivalents, biosurfactants are environment-friendly, biodegradable, less toxic, better foaming properties and non-hazardous. In addition, they are active at extreme temperatures, pH and salinity as well (Souza et al 2017). These properties make production of biosurfactants inexpensive and allow utilization of waste substrates; thereby reducing their polluting effect (Mukherjee et al 2006;Cortés-Camargo et al 2016;Patra et al 2009;Pradhan et al 2013;Shoeb et al 2015;Paraszkiewicz et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Screening, isolation and characterization of biosurfactant-producing Bacillus tequilensis strain ANSKLAB04 from brackish river water

Nayarisseri

Singh

2018

Int. J. Environ. Sci. Technol.

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Biosurfactants are amphiphilic compounds produced by micro-organisms that are excreted extracellularly and contain hydrophobic as well as hydrophilic moieties which reduce surface tension. These extracellular membrane vesicles aid in the uptake of organic molecules that are used as a source of energy for growth of micro-organisms. These micro-organisms have the ability to grow and proliferate on the oil-based medium like petro-contaminated soil and water. Oil spills and discharge from industry contaminates the water bodies and surrounding land. Many chemical-based technologies and manual cleaning are involved to reduce water and land pollution. Chemicals used are the surfactants that lead to the generation of toxic by-products. Hence, considerable attention has been given to surface-active molecules of biological origin called biosurfactants, produced by microbial species in and around the contaminated area. The present study focuses on identifying a novel biosurfactant-producing bacterial strain from brackish water. The bacterial consortium was isolated from targeted brackish water site. Each bacterial species was tested for its biosurfactant production ability using various assays. Haemolysis, oil spreading, cetyltrimethyl ammonium bromide agar plate, drop collapse, hydrocarbon overlay agar, hydrocarbon assay and lipase production were a few qualitative methods performed. Further, the most efficient biosurfactant producer was identified by measuring emulsification index, quantifying biosurfactant production and efficient reduction in surface tension. 16S rRNA gene sequencing was performed for phenotypic characterization of the biosurfactant-producing strain, using Sanger dideoxy sequencing method followed by the phylogenetic assessment. The isolate was found to be a novel Bacillus tequilensis strain, named as ANSKLAB04. The novel isolate was deposited in GenBank with Accession number KU529483.

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“…i. e.; Brevibacillus agri, Burkholderia lata and Bacillus licheniformis . The Burkholderia and Bacillus licheniformis , are known to possess toluene degradation ability (26–28). Although a thermophilic bacterium (growth temp.…”

Section: Discussionmentioning

confidence: 99%

Biochemical, molecular and antibiotic resistance profile of multi-potential toluene metabolizing bacteria isolated from tannery effluents

Muccee

Ejaz

2018

Preprint

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16The focus of present study was to isolate and characterize bacteria which can be 17 effectively used for toluene, a highly recalcitrant pollutant, bioremediation. For isolation 18 of bacteria from the tannery effluents selective enrichment and serial dilution methods 19 were employed. The isolated bacteria were subjected to growth curve analysis, estimation 20 of toluene removal efficiencies, biochemical tests, antibiotic sensitivity assays and 21 molecular characterization based upon 16S rRNA gene. The rRNA genes sequences were 22 analyzed through BLAST to determine similarity index of isolates with bacterial database 23 sequences. To trace the evolutionary history, phylogenetic trees were constructed using 24 MEGA version 7. Total twenty toluene metabolizing bacteria (4)(5)(6)(7)(8)(9)(10)(11)(12) 16, 19, 21, 25 23-26, 28 and 30) were isolated and characterized. Their rRNA gene sequences have 26 been submitted to Genbank. Fifteen of the twenty isolates showed homology to 27 2 Brevibacillus agri strain NBRC 15538, four found similar to Bacillus paralicheniformis 28 strain KJ-16 and one homologous to Burkholderia lata strain 383. All bacterial isolates 29 were resistant to chloramphenicol but sensitive to teicoplanin and linezolid. However, 30 few (i. e.; IUBT9 and 26) were sensitive to oxacillin. Biochemical characterization 31 indicated all bacteria positive for alkaline phosphatases (100%). While many were found 32 positive for p-nitrophenyl N-acetyl β, D-glucosaminidase (35%), hydroxyproline β-33 naphthylaminopeptidase (15%), esculinase (65%), mannitol (75%), sorbitol (95%) and 34 inulin (90%) fermentation. Biochemical profile suggests the use of isolated bacteria for 35 future exploitation in several fields like bioremediation of toluene, ethanol production, 36 biomass hydrolysis, biosensors, biofertilizers, as a marker for milk pasteurization in dairy 37 industries and evaluation of soil quality. 38 Importance 39Toluene is a highly toxic environmental pollutant. We have isolated bacteria which can 40 be effectively used for the removal of toluene from environmental resources. Moreover, 41 these bacteria are capable to produce many valuable enzymes which can be used in many 42 industrial processes for the production of a wide range of products. Further study may 43 help to exploit these bacterial for the benefit of humanity. 44 45 Key words: toluene metabolizing bacteria, bioremediation, toluene removal efficiency, 46 Brevibacillus agri, Bacillus paralicheniformis, Burkholderia lata, rRNA profiling 47 48 49Toluene (methylbenzene) is an omnipresent pollutant and is a cause of concern due to its 50 resistance to chemical, photolytic and biological degradation, lipophilic nature, 51 bioaccumulation, long-range transport and wide range adverse effects on environment, 52 wild life, biota and human health (1). Being lipophilic, it gets accumulated in lipid bilayer 53 of cell membrane and alters the structure of living cells (2). Toluene is a man-made 54 hydrocarbon and its major sources in urban air e...

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Influence of toluene and salinity on biosurfactant production by Bacillus sp.: scale up from flasks to a bench-scale bioreactor

Cited by 10 publications

References 30 publications

Culture Medium Development for Microbial-Derived Surfactants Production—An Overview

Culture Medium Development for Microbial-Derived Surfactants Production—An Overview

Screening, isolation and characterization of biosurfactant-producing Bacillus tequilensis strain ANSKLAB04 from brackish river water

Biochemical, molecular and antibiotic resistance profile of multi-potential toluene metabolizing bacteria isolated from tannery effluents

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