Influence of titania nanotopography on human vascular cell functionality and its proliferation in vitro

Mohan, Chandini C.; Sreerekha, P.R.; Divyarani, V.V.; Nair, Shantikumar V.; Chennazhi, K.P.; Menon, Deepthy

doi:10.1039/c1jm13726c

Cited by 52 publications

(47 citation statements)

References 43 publications

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“…With DES the problems of allergenic reactions as well as risks of restenosis were lowered, as DES release anticell‐proliferative, immunosuppressive or antithrombogenic drugs which inhibit proliferation of smooth muscle cells and reduce thrombus formation . Titanium in its oxide form has been proposed, because of its excellent tissue response, as a suitable stent coating on bare metal stents, providing a hemocompatible surface, with alterations in its surface chemistry and topography contributing to improved biocompatibility . Success of stents depends mainly on avoiding the aggregation of platelets, preventing uncontrolled proliferation of smooth muscle cells and appropriate proliferation of endothelial cells.…”

Section: Introductionmentioning

confidence: 99%

Binding of human coronary artery endothelial cells to plasma‐treated titanium dioxide nanotubes of different diameters

Flašker

Kulkarni

Mrak-Poljšak

et al. 2016

J Biomedical Materials Res

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Nanoscale topography in improving vascular response in vitro was established previously on various titanium surfaces. In the present study different surface nanotopographies that is different diameters of titanium dioxide (TiO2 ) nanotubes (NTs) were fabricated by electrochemical anodization and conditioned with highly reactive gaseous oxygen plasma. The morphology of different diameter NTs was studied by scanning electron microscopy and atomic force microscopy, while changes in chemical composition on the surface before and after plasma treatment were determined by X-ray photoelectron spectroscopy. Performance of human coronary artery endothelial cells (HCAEC) on those conditioned surfaces was studied in regard to cell proliferation, released IL-6 protein and immunofluorescence microscopy (IFM). We show that HCAEC function is dependent on the diameter of the TiO2 NTs, functioning far less optimally when bound to 100 nm TiO2 NTs as compared to Ti foil, 15 nm NTs or 50 nm NTs. There were improved, morphological cell shape changes, observed with IFM, between HCAEC growing on oxygen-rich plasma-treated versus nontreated 100 nm NTs. These endothelialized conditioned Ti nanosurfaces could elucidate optimization conditions necessary for vascular implants in coronary arteries.

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Section: Introductionmentioning

confidence: 99%

Binding of human coronary artery endothelial cells to plasma‐treated titanium dioxide nanotubes of different diameters

Flašker

Kulkarni

Mrak-Poljšak

et al. 2016

J Biomedical Materials Res

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show abstract

“…Recently, increasing publications have shown that nanoscale topography may interact with a variety of cells more effectively than conventional micro-or macrostructures, [1][2][3][4][5][6] among which TiO 2 nanotubes (NTs) have drawn increasing attention. This nanotubular architecture can be fabricated by simple and economic electrochemical anodization and the dimension of NTs can be precisely controlled.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial activity and cytocompatibility of Cu–Ti–O nanotubes

Hang

Gao

Huang

et al. 2013

J Biomedical Materials Res

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TiO2 nanotubes (NTs) have favorable biological properties, but the poor antibacterial activity limits their application especially in orthopedics fields. In this article, Cu-Ti-O nanotubes with different Cu contents are fabricated on sputtered TiCu films. Scanning electron microscopy reveals the NTs can be formed on sputtered TiCu films when the Cu content is less than 14.6 at %. X-ray photoelectron spectroscopy results indicate the NTs are consist of CuO mixed with TiO2 and the Cu content in NTs decreases dramatically compared with that in TiCu films. Biological experiments show that although these NTs have poor release antibacterial activity, their contact antibacterial activity has proven to be excellent, indicating the NT surface can effectively inhibit biomaterial-associated infections. The cytocompatibility of the NTs is closely related to the Cu content and when its content is relatively low (1.01 at %), there is no appreciable cytotoxicity. So Cu-Ti-O NTs with 1 at % Cu may be suitable to achieve proper antibacterial activity and desired cytocompatibility. The Cu-Ti-O NTs integrate the favorable antibacterial activity of Cu and excellent biological properties of TiO2 NTs therefore have potential applications in orthopedics, dentistry, and other biomedical fields.

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“…For DES, this rate can be considered faster than required, in reference to one month with polymeric coating. This may need more optimization of the system via , for example, using drug carriers or external fields505152. However, other factors may have resulted in such relatively fast rate other than the inherent properties of the NTs system.…”

Section: Resultsmentioning

confidence: 99%

Functional Nanoarchitectures For Enhanced Drug Eluting Stents

Saleh

Gepreel

Allam

2017

Sci Rep

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Different strategies have been investigated to allow for optimum duration and conditions for endothelium healing through the enhancement of coronary stents. In this study, a nanoarchitectured system is proposed as a surface modification for drug eluting stents. Highly oriented nanotubes were vertically grown on the surface of a new Ni-free biocompatible Ti-based alloy, as a potential material for self-expandable stents. The fabricated nanotubes were self-grown from the potential stent substrate, which are also proposed to enhance endothelial proliferation while acting as drug reservoir to hinder Vascular Smooth Muscle Cells (VSMC) proliferation. Two morphologies were synthesized to investigate the effect of structure homogeneity on the intended application. The material was characterized by field-emission scanning electron microscope (FESEM), X-ray diffraction (XRD), Raman spectroscopy, energy dispersive X-ray spectroscopy (EDX), and X-ray photoelectron spectroscopy (XPS). Nanoindentation technique was used to study the mechanical properties of the fabricated material. Cytotoxicity and proliferation studies were performed and compared for the two fabricated nanoarchitectures, versus smooth untextured samples, using in-vitro cultured endothelial cells. Finally, the drug loading capacity was experimentally studied and further supported by computational modeling of the release profile.

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Influence of titania nanotopography on human vascular cell functionality and its proliferation in vitro

Cited by 52 publications

References 43 publications

Binding of human coronary artery endothelial cells to plasma‐treated titanium dioxide nanotubes of different diameters

Binding of human coronary artery endothelial cells to plasma‐treated titanium dioxide nanotubes of different diameters

Antibacterial activity and cytocompatibility of Cu–Ti–O nanotubes

Functional Nanoarchitectures For Enhanced Drug Eluting Stents

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