Influence of time on the genetic heterogeneity of Spanish porcine reproductive and respiratory syndrome virus isolates

Prieto, C.; Vázquez, Ana; Núñez, José I.; Álvarez, E.; Simarro, I.; Castro, José María Albertos

doi:10.1016/j.tvjl.2008.01.005

Cited by 28 publications

(23 citation statements)

References 39 publications

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“…The demonstration of an increasing diversity among the population of PRRS viruses confirms earlier reports (Mateu et al, 2006;Fang et al, 2007;Li et al, 2009;Prieto et al, 2009;Shi et al, 2010) and indicates that challenges for diagnostic and immunisation methods increase accordingly (Labarque et al, 2004;Indik et al, 2005). The suggestion that using an attenuated vaccine may contribute to the emergence of new virus variants (Liu et al, 2011) highlights the need to better understand the dynamics of vaccine virus shedding, evolution, and recombination potential.…”

Section: Discussionsupporting

confidence: 79%

“…A high degree of diversity in the virus population has recently been reported in the United States (Fang et al, 2007), China (Li et al, 2009), Spain (Mateu et al, 2006;Prieto et al, 2009), and eastern Europe (Stadejek et al, 2008). Despite the apparent increase in the number of cases of PRRS in Great Britain, little information has been available regarding the virus population since 1995 (Drew, 1996).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Porcine reproductive and respiratory syndrome virus: Genetic diversity of recent British isolates

Frossard

Hughes

Westcott

et al. 2013

Veterinary Microbiology

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Section: Discussionsupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Porcine reproductive and respiratory syndrome virus: Genetic diversity of recent British isolates

Frossard

Hughes

Westcott

et al. 2013

Veterinary Microbiology

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“…Furthermore, the fact that the number of RFLP patterns found in isolates of recent years is higher than in those from early years, after the description of the disease, indicates a continuous genetic drift that increases genetic diversity. This scenario has been previously documented (Wesley et al, 1999;Prieto et al, 2009). …”

Section: Differentiation Of Type-i Prrsv Vaccines and Field Strains Bsupporting

confidence: 71%

“…The RT-PCR was performed using a commercial onestep RT-PCR kit (GeneAmp® Gold RNA PCR Core kit, Applied Biosystems, Foster City, CA, USA), following the manufacturer´s instructions. Primers used to amplify the whole ORF5 and the flanking regions of ORF4 and ORF6 were previously reported by Prieto et al (2009). Second round PCR amplification was performed following a preceding technique (Suárez et al, 1994).…”

Section: Differentiation Of Type-i Prrsv Vaccines and Field Strains Bmentioning

confidence: 99%

Short communication. Differentiation of Type-I Porcine Reproductive and Respiratory Syndrome Virus vaccines and field strains by restriction fragment length polymorphism analysis

Díez-Fuertes

Vázquez

Hornillos-Gumiel

et al. 2012

Span. j. agric. res.

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The use of modified live virus (MLV) vaccines is a common procedure to control porcine reproductive and respiratory syndrome virus (PRRSV) infection in the great majority of countries from America, Asia and Europe, including Spain. Current discriminatory techniques allow the detection of different MLV type-II vaccine strains. Herein we report a rapid and accurate technique aimed to discriminate between MLV type-I vaccine strains and Spanish field strains. This technique comprises a reverse transcription (RT) and nested polymerase chain reaction (nPCR) amplification of PRRSV ORF5 followed by a digestion of RT-nPCR products with two specific endonucleases, ItaI and AccI. Combined utilization of ItaI and AccI generates restriction fragments length polymorphisms (RFLP) patterns adequate for the differentiation of 30 Spanish field isolates, of which 12 were isolated between 1991 and 1995 and 18 between 2000 and 2003. These different RFLP patterns can be used to distinguish unequivocally between Spanish field strains of PRRSV and the three MLV type-I vaccines used in Spain: AmervacPRRS®, Pyrsvac-183® and PorcilisPRRS®.

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“…Korean type I PRRSVs were related with type I PRRSVs from Western Europe, North America, and Thailand. Notably, Korean type I PRRSVs were grouped as a unique cluster, along with a PRRSV of Spanish origin [26]. The sequence identity showed that Korean type I isolates were 90.2-91.4% of identity with the Spanish type I PRRSV .…”

Section: Phylogenetic Analysis Of Orf5mentioning

confidence: 98%

Prevalence and phylogenetic analysis of the isolated type I porcine reproductive and respiratory syndrome virus from 2007 to 2008 in Korea

Lee¹,

Kim

Kang

et al. 2010

Virus Genes

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The first Korean strain of porcine reproductive and respiratory syndrome virus (PRRSV) was isolated in 1997, and it exhibited high similarity to strain VR-2332 (type II PRRSV; North American type). Recently, however, infection with type I PRRSV (European type) has also been reported in Korea. To date, preliminary data about type I PRRSV prevalence in Korea have not been reported. Here, using reverse transcriptase (RT)-PCR, we analyzed 383 archived field samples from 101 pig farms in Korea that were collected from 2007 to 2008. We identified 155 samples from 68 farms that were positive for PRRSV. Fifty-one samples (51/155; 32.9%) and 20 farms (20/68; 29.4%) were type I PRRSV-positive/type II PRRSV-negative. Furthermore, we tried to isolate the type I PRRSV from positive samples and seven type I PRRSV were isolated using PAM. The phylogenetic analysis using the type I PRRSV isolates (7 isolates) was performed based on open reading frame (ORF)5 (accession numbers GU325642 to GU325648) and ORF7 (accession numbers GU325635 to GU325641). In the phylogenetic study, seven type I PRRSV isolates were closely related with panEuropean based on ORF7, while they were genetically distinct from Lelystad virus and made a unique clade based on ORF5. The results of this study demonstrate that infection with type I PRRSV is not uncommon in Korean pig farms, which suggests that diagnosis and control of type I PRRSV should be considered in Korea. A new approach to vaccination against, and epidemiological analysis of, Korean PRRSV is urgently needed.

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Influence of time on the genetic heterogeneity of Spanish porcine reproductive and respiratory syndrome virus isolates

Cited by 28 publications

References 39 publications

Porcine reproductive and respiratory syndrome virus: Genetic diversity of recent British isolates

Porcine reproductive and respiratory syndrome virus: Genetic diversity of recent British isolates

Short communication. Differentiation of Type-I Porcine Reproductive and Respiratory Syndrome Virus vaccines and field strains by restriction fragment length polymorphism analysis

Prevalence and phylogenetic analysis of the isolated type I porcine reproductive and respiratory syndrome virus from 2007 to 2008 in Korea

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