Background : Tibial rotation in total knee arthroplasty remains controversial. The aim was to investigate which anatomical reference was closer to SEA in 10 tibial markers in Chinese adult. Methods : The study included 122 normal lower extremities. 10 axes were drawn on the axial sections: transverse axis of tibia(TAT), axis of medial edge of patellar tendon (MEPT), axis of medial 1⁄3 of patellar tendon attachment (M1/3), Akagi line, Insall line, axis of medial border of tibial tubercle(MBTT),axis of anterior border of the tibia 1-4(ATC1-4). The angles between TAT and SEA as well as the angles between other axes and the perpendicular to SEA were measured. Pairwise differences among the 10 tibial axes were examined using one-way ANOVA and paired t-test. Results : In all the knees, the mean angles of M1/3, Akagi line, Insall line, MBTT, ATC1, ATC2, ATC3, and ATC4 axes compared to perpendicular of projected SEA were 10.2±5.1°, 1.4±5.0°, 11.9±5.4°, 3.6±4.8°, 12.0±6.9°, 7.2±8.6°, 7.1±10.4°, and 6.6±13.5° external rotation, respectively, and the MEPT axis was 1.6±4.5° internal rotation. The TAT was 4.1±5.3° external rotation compared to SEA. The M1⁄3 and Insall line were significantly externally rotated than Akagi line, MEPT, MBTT, TAT, ATC2, ATC3, and ATC4 axes. There was no significant difference between the TAT axis and the MBTT axis, as well as no significant difference between the ATC2, ATC3, and ATC4 axes. Conclusion : Akagi line, MBTT and TAT show good consistency with SEA in axial femorotibial alignment with knee in extension. The middle segment of the anterior tibial crest also has good alignment consistency with SEA in axial femorotibial alignment, it can be reliable reference mark for rotational alignment of the tibial component in TKA.