Influence of thyroid hormone status on expression of genes encoding G protein subunits in the rat heart.

Levine, Michael A.; Feldman, Arthur M.; Robishaw, Janet D.; Ladenson, Paul W.; Ahn, Thomas G.; Moroney, John F.; Smallwood, Philip M.

doi:10.1016/s0021-9258(19)39805-9

Cited by 76 publications

(3 citation statements)

References 49 publications

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“…Rats with chemically induced hyperthyroidism did not have alterations in G s , G i2 , or G i3 in their ventricular tissue. 32 However, in another study, 33 expression of G i1 and G i2 were decreased or unchanged in tissues of the heart and vas deferens and unchanged in the cerebral cortex or liver, but expression of G s was increased in cardiac tissues. Rats treated with triiodothyronine to induce hyperthyroidism had reduced expression of G i in adipose cells.…”

Section: Discussionmentioning

confidence: 87%

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Altered expression of G proteins in thyroid gland adenomas obtained from hyperthyroid cats

Hammer¹,

Holt²,

Ward³

2000

ajvr

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Section: Discussionmentioning

confidence: 87%

“…Median values are reported, and the error bar represents the 75th percentile value. 32 P NAD, PTX radiolabeling was seen at 41 kd, corresponding to G iα . This radiolabeling confirmed the result obtained from immunoblot analysis in that there visually appeared to be less G iα in hyperthyroid membranes than in euthyroid membranes (Fig 7).…”

Section: Resultsmentioning

confidence: 99%

Altered expression of G proteins in thyroid gland adenomas obtained from hyperthyroid cats

Hammer¹,

Holt²,

Ward³

2000

ajvr

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“…Moreover, the distribution of the G protein p subunit genes over three chromosomes suggests that coordinate expression may be regulated by homologous tram-acting factors rather that by a common ci.s regulatory mechanism. Indeed, the parallel increase in expression of the genes encoding the 81 and fi2 [and Gi42) and G,&(3)] proteins in response to hypothyroidism suggests that there is coordinate regulation of these genes by thyroid hormone (Levine et al, 1990a).…”

Section: Discussionmentioning

confidence: 99%

Chromosomal localization of the genes encoding two forms of the G protein β polypeptide, β1 and β3, in man

1990

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The signal-transducing G proteins are heterotrimers composed of three subunits, alpha, beta, and gamma. Multiple distinctive forms of the alpha, beta, and gamma subunits, each encoded by a distinct gene, have been described. To investigate further the structural diversity of the beta subunits, we recently cloned and characterized a novel cDNA encoding a third form of the G protein beta subunit, which we have termed beta 3. The protein corresponding to beta 3 has not yet been identified. The three forms of the beta subunit show 81-90% amino acid sequence identity. Previous studies had localized the human genes for the beta 1 and beta 2 subunits to chromosomes 1 and 7, respectively. The present studies were designed to determine whether the gene encoding beta 3 is linked to either the beta 1 or the beta 2 gene. Genomic DNA was isolated from a panel of rodent-human hybrid cell lines and analyzed by hybridization to cDNAs for beta 1 and beta 3. Discordancy analysis allowed assignment of the beta 3 gene to chromosome 12 and confirmed the previous assignment of the beta 1 gene to chromosome 1. These results were confirmed and extended by using in situ chromosome hybridization, which permitted the regional localization of the beta 1 gene to 1pter----p31.2 and the beta 3 gene to 12pter----p12.3. Digestion of human genomic DNA with 10 restriction enzymes failed to disclose a restriction fragment length polymorphism for the beta 3 gene. These data indicate that there is considerable diversity in the genomic organization of the beta subunit family.

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Chronic ethanol consumption disturbs G-protein expression and inhibits cyclic AMP–dependent signaling in regenerating rat liver

et al. 1992

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Evidence suggests that ethanol desensitizes hepatocytes to the trophic effects of hormones. Cyclic AMP-dependent signals are important regulators of intermediary metabolism, cellular proliferation and differentiation, and modulate liver growth during hepatic regeneration. The events leading to cyclic AMP accumulation after partial hepatectomy were characterized in rats consistently fed ethanol-containing diets and compared with results in rats fed isocaloric amounts of nonethanol diet to determine whether altered cyclic AMP-dependent signal transduction contributes to ethanol-associated aberrations in hepatic growth regulation. Ethanol treatment significantly inhibited hepatic accumulation of cyclic AMP after partial hepatectomy. This was most likely the result of decreased synthesis of cyclic AMP because activation of adenylyl cyclase by agents acting through receptors (e.g., glucagon or isoproterenol), GTP-binding proteins (GTP-gamma-S) and directly on adenylyl cyclase (e.g., forskolin) was significantly inhibited in ethanol-fed rats. Both homologous and heterologous desensitization contributed to this effect. beta 1-Adrenergic receptors were relatively down-regulated 6 hr after partial hepatectomy in ethanol-fed rats, whereas glucagon receptor kinetics were similar in the two groups. Liver membrane expression of GTP-binding proteins differed markedly after partial hepatectomy in ethanol-fed and pair-fed rats. Ethanol significantly inhibited post-partial hepatectomy induction of the stimulatory G protein, Gs alpha but led to overexpression of the inhibitory, G(i)2 alpha, subunit. Steady-state messenger RNA levels of these G proteins were similar in ethanol-fed and pair-fed rats, suggesting that ethanol inhibits G protein expression posttranscriptionally.(ABSTRACT TRUNCATED AT 250 WORDS)

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Influence of thyroid hormone status on expression of genes encoding G protein subunits in the rat heart.

Cited by 76 publications

References 49 publications

Altered expression of G proteins in thyroid gland adenomas obtained from hyperthyroid cats

Altered expression of G proteins in thyroid gland adenomas obtained from hyperthyroid cats

Chromosomal localization of the genes encoding two forms of the G protein β polypeptide, β1 and β3, in man

Chronic ethanol consumption disturbs G-protein expression and inhibits cyclic AMP–dependent signaling in regenerating rat liver

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