Influence of three-dimensional scaffold on the expression of osteogenic differentiation markers by human dermal fibroblasts

Hee, Christopher K.; Jonikas, Magdalena; Nicoll, Steven B.

doi:10.1016/j.biomaterials.2005.07.004

Cited by 50 publications

(32 citation statements)

References 73 publications

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“…The ability of the fibroblastic cells to express in vitro the noncollagenous proteins such as OPN was previously reported. 24,25 The data obtained from the in vivo experiments have indicated that the cellular origin of the ECM is not essential for …”

Section: Discussionmentioning

confidence: 97%

Cell-Derived Matrix Enhances Osteogenic Properties of Hydroxyapatite

Tour

Wendel

Tcacencu

2011

Tissue Engineering Part A

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The study aimed to evaluate osteogenic properties of hydroxyapatite (HA) scaffold combined with extracellular matrix (ECM) derived in vitro from rat primary calvarial osteoblasts or dermal fibroblasts. The cellular viability, and the ECM deposited onto synthetic HA microparticles were assessed by MTT, Glycosaminoglycan, and Hydroxyproline assays as well as immunohistochemistry and scanning electron microscopy after 21 days of culture. The decellularized HA-ECM constructs were implanted in critical-sized calvarial defects of Sprague-Dawley rats, followed by bone repair and local inflammatory response assessments by histomorphometry and immunohistochemistry at 12 weeks postoperatively. We demonstrated that HA supported cellular adhesion, growth, and ECM production in vitro, and the HA-ECM constructs significantly enhanced calvarial bone repair (p<0.05, Mann-Whitney U-test), compared with HA alone, despite the significantly increased number of CD68+ macrophages, and foreign body giant cells (p<0.05, Mann-Whitney U-test). Selective accumulation of bone sialoprotein, osteopontin, and periostin was observed at the tissue-HA interfaces. In conclusion, in vitro-derived ECM mimics the native bone matrix, enhances the osteogenic properties of the HA microparticles, and might modulate the local inflammatory response in a bone repair-favorable way. Our findings highlight the ability to produce functional HA-ECM constructs for bone tissue engineering applications.

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Section: Discussionmentioning

confidence: 97%

Cell-Derived Matrix Enhances Osteogenic Properties of Hydroxyapatite

Tour

Wendel

Tcacencu

2011

Tissue Engineering Part A

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“…Specifically, human gingival and dermal fibroblasts were able to express osteoblast differentiation markers after transduction with vectors overexpressing BMPs [38,50,76]. Human dermal fibroblasts cultured on ultraporous b-tricalcium phosphate ceramics demonstrated increased osteogenic differentiation [36]. Phillips et al [73,74] reported primary dermal fibroblasts overexpressing Runx2 can be used as a mineralizing cell source from bone tissue engineering.…”

Section: Design Considerations For Functional Tissue Engineering Of Pmentioning

confidence: 99%

A Perspective: Engineering Periosteum for Structural Bone Graft Healing

Zhang

Awad

O’Keefe

et al. 2008

Clin Orthop Relat Res

205

169

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Autograft is superior to both allograft and synthetic bone graft in repair of large structural bone defect largely due to the presence of multipotent mesenchymal stem cells in periosteum. Recent studies have provided further evidence that activation, expansion and differentiation of the donor periosteal progenitor cells are essential for the initiation of osteogenesis and angiogenesis of donor bone graft healing. The formation of donor cell-derived periosteal callus enables efficient host-dependent graft repair and remodeling at the later stage of healing. Removal of periosteum from bone autograft markedly impairs healing whereas engraftment of multipotent mesenchymal stem cells on bone allograft improves healing and graft incorporation. These studies provide rationale for fabrication of a biomimetic periosteum substitute that could fit bone of any size and shape for enhanced allograft healing and repair. The success of such an approach will depend on further understanding of the molecular signals that control inflammation, cellular recruitment as well as mesenchymal stem cell differentiation and expansion during the early phase of the repair process. It will also depend on multidisciplinary collaborations between biologists, material scientists and bioengineers to address issues of material selection and modification, biological and biomechanical parameters for functional evaluation of bone allograft healing.

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“…Human MSCs, although widely recognized as a unique cellular entity, are observed intriguingly to be similar to dermal fibroblasts with respect to cell size, morphology, growth property, cell surface phenotype, and immunomodulatory function (Lysy et al 2007;Sabatini et al 2005;Haniffa et al 2007). Traditionally, mesodermal differentiation potential has been used to distinguish between MSCs and fibroblasts (Javazon et al 2004), but this line of demarcation has recently lost its clarity since fibroblasts, like MSCs, have been found to differentiate into osteocytes (Hee et al 2006;Phillips et al 2006), chondrocytes (Rutherford et al 2003), adipocytes (Feldon et al 2006), and even hepatocytes (Lysy et al 2007). …”

Section: Introductionmentioning

confidence: 98%

Gene and microRNA expression signatures of human mesenchymal stromal cells in comparison to fibroblasts

Bae¹,

Ahn²,

Park³

et al. 2008

Cell Tissue Res

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Human mesenchymal stromal cells (MSCs) offer great hope for the treatment of tissue degenerative and immune diseases, but their phenotypic similarity to dermal fibroblasts may hinder robust cell identification and isolation from diverse tissue harvests. To identify genetic elements that can reliably discriminate MSCs from fibroblasts, we performed comparative gene and microRNA expression profiling analyses with genome-wide oligonucleotide microarrays. When taken globally, both gene and microRNA expression profiles of MSCs were highly similar to those of fibroblasts, accounting well for their extensive phenotypic and functional overlaps. Scattered expression differences were pooled to yield an MSC-specific molecular signature, consisting of 64 genes and 21 microRNAs whose expressions were at least 10-fold and two-fold higher, respectively, in MSCs compared with fibroblasts. Genes either encoding transmembrane proteins or associated with tumors were relatively abundant in this signature. These data should provide the molecular basis not only for the discovery of novel diagnostic markers discriminating MSCs from fibroblasts, but also for further studies on MSC-specific signaling mechanisms.

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Influence of three-dimensional scaffold on the expression of osteogenic differentiation markers by human dermal fibroblasts

Cited by 50 publications

References 73 publications

Cell-Derived Matrix Enhances Osteogenic Properties of Hydroxyapatite

Cell-Derived Matrix Enhances Osteogenic Properties of Hydroxyapatite

A Perspective: Engineering Periosteum for Structural Bone Graft Healing

Gene and microRNA expression signatures of human mesenchymal stromal cells in comparison to fibroblasts

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