Influence of thoracoscopic operations on pulmonary function and postoperative recovery

Abstract

Background Nowadays, the age of patients qualified for lung surgery has been lowered. These people have increasingly higher requirements for postoperative quality of life, which is closely related to pulmonary function (PF). Therefore, it’s meaningful to analyze the effects of different surgical methods on postoperative PF and postoperative recovery. Methods A total of 171 patients underwent thoracoscopic lung surgery were selected in our study: unilateral lobectomy (UL), unilateral sublobectomy (USL), and other surgical method (OSM). Other operations included unilateral/bilateral lobectomy and/or sublobectomy. Study indicators included patient general condition, PF and recovery condition. Results The USL was in the best condition during and after surgery. Then was OSM, USL was the worst. All the thoracoscopic operations significantly reduced PF and the loss of PF in the UL was significantly higher than USL. In the long-term observation, the loss of PF after lobectomy was almost the same. The compensatory capacity of the upper lobe was better than the lower lobe. The prediction models of PF cannot fully reflect the actual situation after operation. Our PF loss assessment table based on clinical data was constructed to correct existing models. Conclusions Surgeries had significant effects on PF, but these changes gradually disappeared after operation. The greatest affect were PEF and PEF%, the least were MVV and MVV%. The compensatory function of the upper lobe was better than lower one. The loss of PF in multiple lung tissue resection is equivalent to lobectomy. Our postoperative PF assessment table can better reflect the PF than existing prediction models.

Annotations of four high-quality indigenous chicken genomes identify more than one thousand missing genes in sub-telomeric regions with high G/C contents

“…More than one thousand protein-coding genes missed in reference annotations are found in four indigenous chicken genomes By using a combination of homology-based and RNA-seq-based method, we predicted 17,497~17,718 protein-coding genes in each of our recently assembled four indigenous chicken genomes [5] (Table 1). Interestingly, these numbers of annotated genes in the indigenous genomes are similar to those annotated in the RJF genome GRCg6a (17,485), but fewer than those annotated in the broiler GRCg7b (18,024) and the layer GRCg7w (18,016) genomes.…”

“…We recently sequenced and assembled genomes of four indigenous chicken breeds with unique morphological traits from Yunnan province, China, including Daweishan, Hu, Piao and Wuding chicken, using a combination of long reads, short reads, and Hi-C reads [5]. These chromosome-level assemblies are of higher or comparable quality with the recently released chicken reference genomes GRCg7b/w, providing us an opportunity to survey the repertoire of genes, particularly, protein-coding genes encoded in genomes of diverse chicken breeds.…”

Large scale loss-of-function mutations during chicken evolution and domestication

“…Using an annotation pipeline that combines homology-based and RNA-seq-based methods, we found that the Daweishan, Hu, Piao and Wuding chicken genomes encoded 17,718, 17,497, 17,711 and 17,646 protein-coding genes, respectively [27]. Of these genes in the four genomes, a total of 1,420 are not seen in the annotations of the RJF (GRCg6a), the broiler (GRCg7b) and the layer (GRCg7w) assemblies, we thus refer them to as newly annotated genes (NAGs) [27]. Unexpectedly, we also identified a large number of pseudogenes in the Daweishan (747), Hu (606), Piao (682) and Wuding (667) chicken genomes [27].…”

“…Interestingly, most of the NAGs also are either encoded or become pseudogenes in the GRCg6a, GRCg7b, and GRCga7w assemblies. We therefore increase the numbers of both annotated genes and pseudogenes in GRCg6a (18,463 and 542), GRCg7b (19,002 and 474) and GRCg7w (18,978 and 435) [27]. In addition to the varying numbers of genes and pseudogenes, each pair of chicken genomes share 81%-92% of their genes, which diverge only 7,000-500,000 years ago.…”

“…These chicken breeds include Daweishan chicken with a miniature body size, Hu chicken with a large body size and stout legs, Piao chicken with a rumpless trait and Wuding chicken good at running. Using an annotation pipeline that combines homology-based and RNA-seq-based methods, we found that the Daweishan, Hu, Piao and Wuding chicken genomes encoded 17,718, 17,497, 17,711 and 17,646 protein-coding genes, respectively [27]. Of these genes in the four genomes, a total of 1,420 are not seen in the annotations of the RJF (GRCg6a), the broiler (GRCg7b) and the layer (GRCg7w) assemblies, we thus refer them to as newly annotated genes (NAGs) [27].…”