Influence of the Sample Preparation Method in Discriminating Candida spp. Using ATR-FTIR Spectroscopy

Pebotuwa, Savithri; Kochan, Kamila; Peleg, Anton Y.; Wood, Bayden R.; Heraud, Philip

doi:10.3390/molecules25071551

Cited by 17 publications

(12 citation statements)

References 33 publications

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“…A handful of studies have explored the capacity of IR spectroscopy to discriminate between species and strains of various fungi. [168][169][170][171][172][173][174][175][176][177] Of these, very few have focused on the determination of fungi specifically isolated from blood. 173,178 Maquelin et al 178 developed identification models based on ANN and LDS by creating reference libraries of Raman and FT-IR spectra from bacterial and fungal pathogens commonly seen in BSIs.…”

Section: Fungal Infectionsmentioning

confidence: 99%

Infrared Spectroscopy of Blood

et al. 2021

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The magnitude of infectious diseases in the twenty-first century created an urgent need for point-of-care diagnostics. Critical shortages in reagents and testing kits have had a large impact on the ability to test patients with a suspected parasitic, bacteria, fungal, and viral infections. New point-of-care tests need to be highly sensitive, specific, and easy to use and provide results in rapid time. Infrared spectroscopy, coupled to multivariate and machine learning algorithms, has the potential to meet this unmet demand requiring minimal sample preparation to detect both pathogenic infectious agents and chronic disease markers in blood. This focal point article will highlight the application of Fourier transform infrared spectroscopy to detect disease markers in blood focusing principally on parasites, bacteria, viruses, cancer markers, and important analytes indicative of disease. Methodologies and state-of-the-art approaches will be reported and potential confounding variables in blood analysis identified. The article provides an up to date review of the literature on blood diagnosis using infrared spectroscopy highlighting the recent advances in this burgeoning field.

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Section: Fungal Infectionsmentioning

confidence: 99%

Infrared Spectroscopy of Blood

et al. 2021

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“…Over the past decade, both Raman and FTIR spectroscopy have become widely used in microbiology research on fungi [ 6 , 7 , 8 , 9 ] and bacteria [ 10 , 11 , 12 , 13 , 14 , 15 ]. FTIR spectroscopy is commonly applied for bacterial classification, with the ability to reach strain-level discrimination.…”

Section: Introductionmentioning

confidence: 99%

Vibrational Spectroscopy as a Sensitive Probe for the Chemistry of Intra-Phase Bacterial Growth

Kochan

Lai

Richardson

et al. 2020

Sensors

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Bacterial growth in batch cultures occurs in four phases (lag, exponential/log, stationary and death phase) that differ distinctly in number of different bacteria, biochemistry and physiology. Knowledge regarding the growth phase and its kinetics is essential for bacterial research, especially in taxonomic identification and monitoring drug interactions. However, the conventional methods by which to assess microbial growth are based only on cell counting or optical density, without any insight into the biochemistry of cells or processes. Both Raman and Fourier transform infrared (FTIR) spectroscopy have shown potential to determine the chemical changes occurring between different bacterial growth phases. Here, we extend the application of spectroscopy and for the first time combine both Raman and FTIR microscopy in a multimodal approach to detect changes in the chemical compositions of bacteria within the same phase (intra-phase). We found a number of spectral markers associated with nucleic acids (IR: 964, 1082, 1215 cm−1; RS: 785, 1483 cm−1), carbohydrates (IR: 1035 cm−1; RS: 1047 cm−1) and proteins (1394 cm−1, amide II) reflecting not only inter-, but also intra-phase changes in bacterial chemistry. Principal component analysis performed simultaneously on FTIR and Raman spectra enabled a clear-cut, time-dependent discrimination between intra-lag phase bacteria probed every 30 min. This demonstrates the unique capability of multimodal vibrational spectroscopy to probe the chemistry of bacterial growth even at the intra-phase level, which is particularly important for the lag phase, where low bacterial numbers limit conventional analytical approaches.

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“…The point-of-care system, which analysed spectroscopic measurements on a cloud-based system, and subsequently delivered clinical test results to a mobile phone or computer, highlights the accessibility of the technology for mass screening of malaria in resource limited countries. As well as detection of parasites, they have shown the clinical utility of their spectroscopic approach for identification of bacterial and fungal pathogens 192,193 , and hepatitis viruses 75 . In fact, Biotech Resources have recently been awarded an investment from the Australian Government's $35 million BioMedTech Horizons program, for development of a rapid spectroscopic test for direct detection of bacterial and fungal pathogens that cause sepsis in patients from whole blood 194 .…”

Section: Infraredmentioning

confidence: 99%

“…188 The point-of-care system, which analyzed spectroscopic measurements on a cloudbased system, and subsequently delivered clinical test results to a mobile phone or computer, highlights the accessibility of the technology for mass screening of malaria in resource-limited countries. As well as detection of parasites, they have shown the clinical utility of their spectroscopic approach for identification of bacterial and fungal pathogens, 192,193 and hepatitis viruses. 75 The Monash Centre for Biospectroscopy is currently developing a rapid antibiotic susceptibility test capable of detecting resistance to numerous antibiotics in under 2 h from positive blood cultures.…”

Section: Infraredmentioning

confidence: 99%

Clinical Spectroscopy: Lost in Translation?

Cameron¹,

Rinaldi

Rutherford

et al. 2021

Appl Spectrosc

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This focal point article discusses the developments of biomedical Raman and infrared spectroscopy, and the recent strive towards being a recognised clinical tool for various applications. The promise of vibrational spectroscopy in the field of biomedical science, alongside the development of computational methods for spectral analysis, has driven a plethora of proof-of-concept studies which convey the potential of various spectroscopic approaches. Here we report a brief review of the literature published over the past few decades, with a focus on the current technical, clinical and economic barriers to translation, namely the limitations of many of these early studies, the lack of understanding of clinical pathways, health technology assessments, regulatory approval, clinical feasibility and funding applications. The field of biomedical vibrational spectroscopy must acknowledge and overcome these hurdles in order to achieve clinical efficacy. Current prospects have been overviewed with comment on the advised future direction of spectroscopic technologies, with the aspiration that many of these innovative approaches can ultimately reach the frontier of medical diagnostics and other clinical applications.

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Influence of the Sample Preparation Method in Discriminating Candida spp. Using ATR-FTIR Spectroscopy

Cited by 17 publications

References 33 publications

Infrared Spectroscopy of Blood

Infrared Spectroscopy of Blood

Vibrational Spectroscopy as a Sensitive Probe for the Chemistry of Intra-Phase Bacterial Growth

Clinical Spectroscopy: Lost in Translation?

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