Influence of the RF drive potential on the acceptance behavior of pure quadrupole mass filters operated in stability zones A and B

Huntley, Adam P.; Brabeck, Gregory F.; Reilly, Peter T. A.

doi:10.1016/j.ijms.2020.116303

Cited by 5 publications

(11 citation statements)

References 8 publications

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“…Previously, mass selection with DWT has only been experimentally achieved for small, non-native proteins but has been simulated for complexes approaching MDa masses. − , In this work, the mass selection of individual charge states for large protein complexes (>100 kDa) has been effectuated. The overall performance of DWT for isolation of single charge states of C-reactive protein (CRP), membrane protein ammonium transport channel (AmtB), and type 1 chaperonin GroEL is illustrated by data shown in Figure .…”

Section: Resultsmentioning

confidence: 99%

“…The ions exiting q are guided to the second chamber by an octupole ion guide constructed from 3.175 mm rods with a 4.75 mm r 0 measuring 47 mm in length and enter a 250 mm hyperbolic quadrupole (Q) that is maintained at 10 –4 –10 –5 Torr. Q is used to mass-select the ions by digital-waveform technology (DWT) as described and developed by the Reilly laboratory. − Both quadrupoles are identical Thermo Fisher 4 mm r 0 hyperbolic rods with pre- and postfilters (Part Number 80100–60109); quadrupole pre- and postfilters were wired directly to the main rods prior to use. All the experiments described below employ the DWT operation of the Q MS. Ions exiting Q enter an octupole ion guide (47 mm) and then transfer to a 55 cm PF-DT maintained at pressures of 1–2 Torr of He.…”

Section: Methodsmentioning

confidence: 99%

“…The first quadrupole (q) is operated as an ion guide (RF-only mode) and can also be used for mild collisional activation of the ions to remove unwanted adducted species (H 2 O, salts, detergents, and charge reducing reagents). The second quadrupole (Q) is used for mass selection and is operated using digital-waveform technology (DWT) developed in the Reilly laboratory. − The modular design of the instrument allows for the installation of additional ion activation techniques, such as surface-induced dissociation (SID), − electron transfer/capture dissociation (ETD/ECD), and ultraviolet photodissociation (UVPD) , prior to IM-MS characterization. Collectively, the experimental versatility of the (vT)-nESI-qQ-PF-DT-FT-IM-Orbitrap increases our research capabilities for native-mass-spectrometry-guided structural biology.…”

Section: Introductionmentioning

confidence: 99%

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Implementing Digital-Waveform Technology for Extended m/z Range Operation on a Native Dual-Quadrupole FT-IM-Orbitrap Mass Spectrometer

McCabe

Jones

Walker

et al. 2021

J. Am. Soc. Mass Spectrom.

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Here, we describe a digital-waveform dual-quadrupole mass spectrometer that enhances the performance of our drift tube FT-IMS high-resolution Orbitrap mass spectrometer (MS). The dual-quadrupole analyzer enhances the instrument capabilities for studies of large protein and protein complexes. The first quadrupole (q) provides a means for performing low-energy collisional activation of ions to reduce or eliminate noncovalent adducts, viz., salts, buffers, detergents, and/or endogenous ligands. The second quadrupole (Q) is used to mass-select ions of interest for further interrogation by ion mobility spectrometry and/or collision-induced dissociation (CID). Q is operated using digital-waveform technology (DWT) to improve the mass selection compared to that achieved using traditional sinusoidal waveforms at floated DC potentials (>500 V DC). DWT allows for increased precision of the waveform for a fraction of the cost of conventional RF drivers and with readily programmable operation and precision (Hoffman, N. M. et al.. A comparison-based digital-waveform generator for high-resolution duty cycle. Review of Scientific Instruments 2018, 89, 084101).

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Implementing Digital-Waveform Technology for Extended m/z Range Operation on a Native Dual-Quadrupole FT-IM-Orbitrap Mass Spectrometer

McCabe

Jones

Walker

et al. 2021

J. Am. Soc. Mass Spectrom.

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“…12 As the DC potential and hence the a value increase, the resolving power increases while Δq and the acceptance aperture along each axis decreases. 12,13 AD was determined as a function of resolving power by Pedder over a wide range, R BL = 10 to 4000, for each axis. 9 This acceptance data for the y-axis is plotted in Figure 9 and is depicted as solid red squares.…”

Section: Acceptance and Acceptance Aperturementioning

confidence: 99%

“…When a DC potential is applied between the orthogonal rod sets to create a mass filter, the acceptance ellipses narrow and elongate along one axis thereby reducing the acceptance aperture 12 . As the DC potential and hence the a value increase, the resolving power increases while Δ q and the acceptance aperture along each axis decreases 12,13 . AD was determined as a function of resolving power by Pedder over a wide range, R BL = 10 to 4000, for each axis 9 .…”

Section: Transmission and Its Limitationsmentioning

confidence: 99%

On the relationships between resolution, dimensionless stability, pseudopotential well depth, acceptance, and transmission in mass filters

Huntley

Reilly

2022

J Mass Spectrom

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The relationships between resolution, stability, pseudopotential well depth, acceptance aperture, and transmission for sinusoidal quadrupole mass filters are examined graphically and mathematically. Simple linear or power relationships are revealed.Comparison of these quantities plotted against resolving power show that the pseudopotential well depth correlates well with the mass filter transmission.Pseudopotential well depth is directly proportional to the product of dimensionless stability well depth and the AC voltage. This relationship extends to all quadrupoles regardless of operational zone because it is rooted in stability. Ion transmission and sensitivity scale directly with the pseudopotential well depth. Resolving power and pseudopotential well depth increase when operating in higher stability zones for all types of mass filters. Unfortunately, for fixed frequency sine wave mass filters, the increased resolving power and pseudopotential well depth are accompanied by a significant reduction of the mass range and increased in fringe field effects. For these reasons, sine mass filter operation in higher stability zones has been reported but not commercially produced. In contrast, for rectangular wave mass filter operation, there is no mass range limitation in any stability zone. The fringe field does not increase because the AC voltage is constant and does not change within a single stability zone or between them. A DC voltage is also unnecessary to access any zone. The high resolution and sensitivity of rectangular wave mass filters that can be gained by operation in higher stability zones without mass limit and limited fringe field restrictions suggest a bright and expansive future for this technology.

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Tandem Mass-Selective Cryogenic Digital Ion Traps for Enhanced Cluster Formation

Roesch,

Garand

2023

J. Phys. Chem. A

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We present the implementation of tandem mass-selective cryogenic ion traps, designed to enhance the range of ion processing capabilities that can be performed prior to spectroscopic interrogation. We show that both the formation of ion clusters and mass filtering steps can be combined in a single cryogenic linear quadrupole ion trap driven by RF square waves. Mass filtering and mass isolation can be achieved by manipulation of the RF frequency and duty cycle. Very importantly, this scheme circumvents the need for high-amplitude RF voltages that can be incompatible with typical cryogenic ion processing conditions. In addition, proper adjustment of the stability boundaries during the clustering process allows for the preferential formation of a specific cluster size rather than a broad distribution of sizes. Lastly, we show that a specific cluster size can be formed, mass-selected, and then transferred to another ion trap for a second completely separate ion processing step. The instrumentation and modular design developed here expand the scope of ionic species and clusters that can be accessed by processing electrosprayed ions.

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Influence of the RF drive potential on the acceptance behavior of pure quadrupole mass filters operated in stability zones A and B

Cited by 5 publications

References 8 publications

Implementing Digital-Waveform Technology for Extended m/z Range Operation on a Native Dual-Quadrupole FT-IM-Orbitrap Mass Spectrometer

Implementing Digital-Waveform Technology for Extended m/z Range Operation on a Native Dual-Quadrupole FT-IM-Orbitrap Mass Spectrometer

On the relationships between resolution, dimensionless stability, pseudopotential well depth, acceptance, and transmission in mass filters

Tandem Mass-Selective Cryogenic Digital Ion Traps for Enhanced Cluster Formation

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